Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACLKM1_RS12115 Genome accession   NZ_CP183903
Coordinates   2524935..2526647 (+) Length   570 a.a.
NCBI ID   WP_005094394.1    Uniprot ID   A0A2H9YRE2
Organism   Acinetobacter pseudolwoffii strain M5_NDM     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2519935..2531647
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM1_RS12070 nusA 2520413..2521897 (-) 1485 WP_005094384.1 transcription termination factor NusA -
  ACLKM1_RS12075 rimP 2521932..2522456 (-) 525 WP_005094386.1 ribosome maturation factor RimP -
  ACLKM1_RS12105 secG 2523523..2523852 (-) 330 WP_005094388.1 preprotein translocase subunit SecG -
  ACLKM1_RS12110 tpiA 2523866..2524657 (-) 792 WP_005168944.1 triose-phosphate isomerase -
  ACLKM1_RS12115 pilB 2524935..2526647 (+) 1713 WP_005094394.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLKM1_RS12120 pilC 2526679..2527908 (+) 1230 WP_100329414.1 type II secretion system F family protein Machinery gene
  ACLKM1_RS12125 pilD 2527908..2528768 (+) 861 WP_074383650.1 prepilin peptidase Machinery gene
  ACLKM1_RS12130 coaE 2528770..2529369 (+) 600 WP_074383651.1 dephospho-CoA kinase -
  ACLKM1_RS12135 - 2529391..2530314 (-) 924 WP_005094400.1 DMT family transporter -
  ACLKM1_RS12140 rlmB 2530321..2531070 (-) 750 WP_005168959.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACLKM1_RS12145 - 2531207..2531530 (-) 324 WP_004281089.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63731.98 Da        Isoelectric Point: 4.7621

>NTDB_id=1107099 ACLKM1_RS12115 WP_005094394.1 2524935..2526647(+) (pilB) [Acinetobacter pseudolwoffii strain M5_NDM]
MTALQGSPRFTGFIRRLVEEGVISAEDMRSALAHAKQEKIDIVAELINQQQLSPTVIAETISVEFGEPLFDISAYDPAQI
LRDIIDEKLITKHRILPIFKNSSILYIAISNPTNIEAIDAVRFSTKLNIETIIVEHNKLEKLIEQNFTEESTFEFDEDFD
LDVDFDSSDPSKEEDEKDKGEEEAPIVKYINKLLIDAIRMGASDLHFEPYEKIYRVRYRVDGVLRQIATPPLQLATRLSS
RLKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKELFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKPADIPQQSLLEMGF
TEADLQQPEFQIYEPVGCNECREGYKGRVGVYEVMKVTPEISRIIMEDGNALEIADASARAGFNNLRRSGLIKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1107099 ACLKM1_RS12115 WP_005094394.1 2524935..2526647(+) (pilB) [Acinetobacter pseudolwoffii strain M5_NDM]
ATGACAGCATTACAGGGGTCGCCAAGATTTACCGGATTTATTCGTCGTTTGGTCGAGGAAGGCGTCATCTCGGCTGAAGA
TATGCGCAGTGCTTTGGCACATGCCAAGCAAGAAAAAATAGATATTGTGGCGGAGCTGATTAACCAGCAACAGCTCTCCC
CAACCGTTATTGCTGAAACGATTTCAGTCGAATTTGGTGAGCCGCTGTTTGATATCAGTGCCTATGATCCTGCGCAAATT
TTGCGGGATATCATTGATGAGAAGCTGATTACCAAGCATCGCATCCTGCCAATTTTTAAAAATTCCAGTATTTTATATAT
TGCCATCAGTAATCCGACCAATATTGAAGCGATTGATGCCGTTCGCTTTTCAACCAAGCTCAATATTGAAACCATTATTG
TTGAACACAATAAACTGGAAAAACTGATCGAGCAGAATTTTACCGAAGAAAGCACTTTTGAGTTTGATGAAGACTTTGAT
CTGGATGTAGATTTCGATTCATCTGATCCGAGTAAAGAAGAAGATGAAAAAGACAAAGGTGAAGAAGAAGCACCGATTGT
CAAATATATCAATAAATTATTAATCGATGCGATTCGTATGGGTGCTTCGGATCTGCATTTTGAACCTTATGAAAAAATCT
ACCGGGTTCGTTATCGGGTCGATGGGGTATTACGCCAAATTGCAACACCGCCGCTGCAGTTGGCAACCCGCCTGTCTTCA
CGTTTAAAAGTCATGTCGCAAATGGACATTTCTGAAAAACGTGTTCCTCAGGATGGCCGTATCAAGCTGAAACTCTCAAA
GAACAAAGCCATTGATTTCCGTGTCAACTCATTACCGACCCTGTTTGGCGAAAAACTGGTACTGCGTATTCTAGATCCAT
CTAGCGCGATGCTGGGGATCGATGCACTCGGTTATGAACCGGAACAAAAAGAACTGTTTATGCAGGCGCTGGATAAACCG
CAAGGCATGCTGCTGATTACCGGGCCAACGGGTTCTGGTAAAACGGTTTCACTTTATACCGGTTTGAACATCCTGAATCG
TGAAGATACCAATATTTCCACCGCGGAAGATCCGGTCGAGATCAACCTGCAAGGAATTAACCAGGTCAACGTCAACAATA
AAGTCGGCCTGACCTTCTCTGCTGCCTTGAAGTCATTCTTACGCCAAGATCCAGATATCGTGATGGTCGGTGAGATCCGG
GATCTGGAAACTGCAGAAATTGCCATTAAAGCGGCACAGACCGGTCATATGGTGATGTCAACGCTGCATACCAACAGTGC
GCCTGAGACCCTGACCCGTTTACGCAATATGGGCGTGCCGTCTTTTAATATTGCCACTTCAGTGAATCTGGTTATTGCAC
AACGACTGGCACGTCGCCTGTGTTCACAATGTAAAAAACCTGCCGACATTCCACAGCAGAGTTTATTGGAAATGGGTTTT
ACCGAAGCAGATCTCCAGCAACCAGAGTTCCAGATCTATGAGCCGGTAGGTTGCAATGAATGTCGCGAAGGCTATAAGGG
TCGTGTAGGTGTTTATGAAGTCATGAAAGTGACGCCTGAAATTTCTAGAATTATTATGGAAGATGGCAATGCACTTGAAA
TTGCCGATGCTTCTGCCCGTGCAGGTTTTAACAATTTACGCCGATCAGGTTTAATCAAGGTGATGCAGGGGGTGACTTCT
TTACAGGAAGTCAATCGTGTCACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2H9YRE2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.86

100

0.8
  pilB Acinetobacter baylyi ADP1

76.708

100

0.768
  pilB Legionella pneumophila strain ERS1305867

51.59

99.298

0.512
  pilF Neisseria gonorrhoeae MS11

49.11

98.596

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

48.474

97.719

0.474
  pilB Vibrio campbellii strain DS40M4

48.115

97.719

0.47
  pilB Vibrio cholerae strain A1552

50.192

91.228

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

34.91

100

0.375
  pilF Thermus thermophilus HB27

36.926

99.298

0.367
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.204

100

0.363

Multiple sequence alignment