Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACLKM1_RS12125 Genome accession   NZ_CP183903
Coordinates   2527908..2528768 (+) Length   286 a.a.
NCBI ID   WP_074383650.1    Uniprot ID   A0A2H9YR93
Organism   Acinetobacter pseudolwoffii strain M5_NDM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2522908..2533768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM1_RS12105 secG 2523523..2523852 (-) 330 WP_005094388.1 preprotein translocase subunit SecG -
  ACLKM1_RS12110 tpiA 2523866..2524657 (-) 792 WP_005168944.1 triose-phosphate isomerase -
  ACLKM1_RS12115 pilB 2524935..2526647 (+) 1713 WP_005094394.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLKM1_RS12120 pilC 2526679..2527908 (+) 1230 WP_100329414.1 type II secretion system F family protein Machinery gene
  ACLKM1_RS12125 pilD 2527908..2528768 (+) 861 WP_074383650.1 prepilin peptidase Machinery gene
  ACLKM1_RS12130 coaE 2528770..2529369 (+) 600 WP_074383651.1 dephospho-CoA kinase -
  ACLKM1_RS12135 - 2529391..2530314 (-) 924 WP_005094400.1 DMT family transporter -
  ACLKM1_RS12140 rlmB 2530321..2531070 (-) 750 WP_005168959.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACLKM1_RS12145 - 2531207..2531530 (-) 324 WP_004281089.1 pyrimidine/purine nucleoside phosphorylase -
  ACLKM1_RS12150 recN 2531945..2533606 (+) 1662 WP_411689665.1 DNA repair protein RecN -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32071.27 Da        Isoelectric Point: 7.8044

>NTDB_id=1107101 ACLKM1_RS12125 WP_074383650.1 2527908..2528768(+) (pilD) [Acinetobacter pseudolwoffii strain M5_NDM]
MQDLIQYFIQNPTALYSAVGLFSLCIGSFLNVVIYRTPKMMEQEWRTDCQMFLHPEQPIIDETKLSLSKPDSTCPKCHQP
IRWYQNIPVISWLVLRGKCGNCQNPISIRYPFIELLTAACALMVVAVFGPTVQMLFGLILTYVLIALTFIDFDTQLLPDR
YTLPLAALGLGVNSYALYTSPSSAIWGYIIGFLCLWVVYYVFKIVTGKEGMGYGDFKLLAALGAWMGPLLLPLIVLLSSL
VGAIIGIILLKVRKENQPFAFGPYIAIAGWIAFLWGEQIMKVYLGQ

Nucleotide


Download         Length: 861 bp        

>NTDB_id=1107101 ACLKM1_RS12125 WP_074383650.1 2527908..2528768(+) (pilD) [Acinetobacter pseudolwoffii strain M5_NDM]
ATGCAAGATCTTATTCAATATTTCATTCAAAACCCAACAGCGCTGTATAGCGCAGTTGGGCTTTTTAGTCTCTGCATTGG
TAGTTTCTTAAATGTGGTGATTTATCGCACTCCCAAGATGATGGAGCAGGAATGGCGTACCGATTGCCAAATGTTCCTGC
ATCCGGAACAGCCAATTATTGATGAGACCAAATTAAGTTTAAGCAAACCCGATTCGACTTGCCCCAAATGTCATCAGCCA
ATCCGCTGGTACCAGAATATTCCCGTCATCAGCTGGCTGGTCTTGCGCGGCAAGTGCGGCAACTGTCAAAATCCCATCAG
TATCCGCTATCCATTTATTGAGCTTCTGACCGCGGCCTGTGCGCTGATGGTGGTGGCTGTTTTTGGTCCGACTGTCCAGA
TGTTATTTGGCCTGATTCTGACTTATGTCTTAATTGCACTGACCTTTATTGATTTTGACACCCAGTTATTGCCGGACCGT
TATACCCTACCCCTGGCCGCGCTGGGTCTGGGCGTCAATAGTTATGCCCTCTATACCTCACCGAGTTCTGCCATCTGGGG
CTATATCATTGGCTTCCTGTGTTTGTGGGTGGTGTATTATGTCTTTAAAATTGTCACTGGCAAGGAAGGTATGGGCTATG
GCGATTTTAAATTGCTTGCCGCCTTGGGTGCCTGGATGGGGCCTTTGCTATTACCATTAATTGTGCTCCTTTCTTCTTTA
GTGGGTGCGATTATCGGGATTATCCTGCTGAAAGTCCGTAAGGAAAATCAGCCTTTTGCCTTTGGTCCCTATATCGCGAT
TGCCGGCTGGATTGCTTTTCTGTGGGGCGAGCAGATTATGAAAGTGTATTTAGGTCAATAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(139-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2H9YR93

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

82.105

99.65

0.818
  pilD Acinetobacter baumannii D1279779

81.754

99.65

0.815
  pilD Vibrio cholerae strain A1552

55.431

93.357

0.517
  pilD Vibrio campbellii strain DS40M4

52.809

93.357

0.493
  pilD Neisseria gonorrhoeae MS11

48.485

92.308

0.448

Multiple sequence alignment