Detailed information NTG1107098

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ACLKM1_RS11900
• Genome accession: NZ_CP183903
• Coordinates: 2480936..2481994 (-)
• Length: 352 a.a.
• NCBI ID: WP_005168888.1
• Uniprot ID: N9KWF8
• Organism: Acinetobacter pseudolwoffii strain M5_NDM
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2475936..2486994

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLKM1_RS11875	aroK	2476318..2476854 (-)	537	WP_180186049.1	shikimate kinase AroK	-
ACLKM1_RS11880	pilQ	2476891..2479044 (-)	2154	WP_411689826.1	type IV pilus secretin PilQ	Machinery gene
ACLKM1_RS11885	pilP	2479061..2479588 (-)	528	WP_005094322.1	pilus assembly protein PilP	Machinery gene
ACLKM1_RS11890	comO	2479588..2480301 (-)	714	WP_005094323.1	type 4a pilus biogenesis protein PilO	Machinery gene
ACLKM1_RS11895	comN	2480298..2480936 (-)	639	WP_005094324.1	PilN domain-containing protein	Machinery gene
ACLKM1_RS11900	pilM	2480936..2481994 (-)	1059	WP_005168888.1	pilus assembly protein PilM	Machinery gene
ACLKM1_RS11905	ponA	2482160..2484688 (+)	2529	WP_286412290.1	penicillin-binding protein PBP1a	-
ACLKM1_RS11910	-	2484752..2485555 (-)	804	WP_005094332.1	putative RNA methyltransferase	-
ACLKM1_RS11915	mraY	2485610..2486728 (-)	1119	WP_005094334.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39045.62 Da
• Isoelectric Point: 4.6364

>NTDB_id=1107098 ACLKM1_RS11900 WP_005168888.1 2480936..2481994(-) (pilM) [Acinetobacter pseudolwoffii strain M5_NDM]
MRRLYRKPNKGLIGVDISSTSVKVLELSVKSSRYWVESYALVPLMENSVVEKNVLNPEAVADALGRAINLANTQSNHAAF
AIPTSMVITKTIEMDADMSNDEREVQIREDAEQYIPFPLDEASLDFEVLPDRLANPNRVNVLLVATRIENIEARSEVLEI
AGLTPKIADVESFALENAFKVFSDTLPMGVNTVGILDIGHSMTTLSVMQNNKIIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKTRALPDDYDIEVLEPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQIDIKKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=1107098 ACLKM1_RS11900 WP_005168888.1 2480936..2481994(-) (pilM) [Acinetobacter pseudolwoffii strain M5_NDM]
GTGCGCAGGTTATATCGTAAACCAAATAAGGGGTTAATAGGAGTTGATATTAGTTCGACTTCTGTTAAGGTTTTGGAGCT
TTCTGTAAAAAGTAGCCGTTACTGGGTAGAGAGCTATGCTTTGGTCCCATTAATGGAAAACAGTGTTGTTGAAAAAAACG
TCTTAAACCCGGAAGCAGTCGCGGATGCCCTTGGGCGTGCAATTAATTTGGCCAACACCCAGTCGAATCATGCCGCTTTT
GCCATTCCAACCTCTATGGTGATTACCAAAACTATCGAGATGGATGCAGACATGTCGAATGATGAGCGTGAAGTTCAGAT
TCGTGAGGATGCAGAACAGTATATTCCGTTCCCACTCGATGAAGCCAGTCTGGACTTTGAAGTTCTGCCTGACCGTCTTG
CCAATCCAAACCGGGTCAATGTGCTGTTGGTGGCAACCCGCATTGAAAATATTGAAGCACGTTCTGAAGTTTTGGAAATT
GCCGGACTGACACCCAAGATCGCGGATGTAGAAAGTTTTGCGCTGGAAAATGCTTTTAAAGTGTTTTCGGATACTTTGCC
GATGGGGGTAAATACTGTCGGTATTTTAGACATTGGTCATAGTATGACCACCTTGTCTGTCATGCAGAACAACAAAATTA
TTTATACCCGTGAGCAGGTGTTTGGTGGCAAACAGCTAACTCAGGAAATTCAGAACCGTTATGGTCTGTCTTTTGAAGAA
GCCGGGCGTGCTAAAAAAACCCGTGCTTTGCCAGATGACTATGATATTGAAGTGCTAGAACCGTTCCTGGATGCCGTTGT
GCAACAGGCAGCGCGGTCATTACAATTCTTCTTTTCATCGTCTCAGTTTAATGAGATTGATCATATTTTATTGGCCGGCG
GGAATGCCAATATTCCAGGTCTGGCAAAATTATTACAACAAAAACTCGGTTACCGTGTCACGATCGCGAATCCATTTTTG
CAAATGGGCTTTTCTCCTCAAATTGATATTAAAAAAATTGAAAATGACGCGTCATCACTCATGGTTGCATGTGGTTTGGC
ATTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9KWF8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	85.511	100	0.855
comM	Acinetobacter nosocomialis M2	84.943	100	0.849
comM	Acinetobacter baylyi ADP1	79.545	100	0.795
pilM	Legionella pneumophila strain ERS1305867	39.776	100	0.403