Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACLKM4_RS01500 Genome accession   NZ_CP183895
Coordinates   319803..321029 (-) Length   408 a.a.
NCBI ID   WP_016659656.1    Uniprot ID   V2UF65
Organism   Acinetobacter indicus strain D1_NDM_tet(X3)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 314803..326029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM4_RS01475 - 316208..316531 (+) 324 WP_005181767.1 pyrimidine/purine nucleoside phosphorylase -
  ACLKM4_RS01480 rlmB 316637..317389 (+) 753 WP_005181769.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACLKM4_RS01485 - 317407..318327 (+) 921 WP_016659657.1 DMT family transporter -
  ACLKM4_RS01490 coaE 318324..318932 (-) 609 WP_005181773.1 dephospho-CoA kinase -
  ACLKM4_RS01495 pilD 318943..319803 (-) 861 WP_005181774.1 prepilin peptidase Machinery gene
  ACLKM4_RS01500 pilC 319803..321029 (-) 1227 WP_016659656.1 type II secretion system F family protein Machinery gene
  ACLKM4_RS01505 pilB 321060..322766 (-) 1707 WP_160243588.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLKM4_RS01510 tpiA 323056..323847 (+) 792 WP_005181780.1 triose-phosphate isomerase -
  ACLKM4_RS01515 secG 323861..324190 (+) 330 WP_411687548.1 preprotein translocase subunit SecG -
  ACLKM4_RS01540 rimP 325059..325583 (+) 525 WP_005181784.1 ribosome maturation factor RimP -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44895.36 Da        Isoelectric Point: 9.9079

>NTDB_id=1106962 ACLKM4_RS01500 WP_016659656.1 319803..321029(-) (pilC) [Acinetobacter indicus strain D1_NDM_tet(X3)]
MAVKKAQMMPTFIYEGIDRKGAKLKGELPARNMALAKVTLRKQGITIKTIRQKKKNILEGLMKKKVSTLDITIFTRQLAT
MMKAGVPLVQSFEIVAEGLENPAMREVVLGIKGEVEGGNTFAGALRKYPQHFDNLFCSLVESGEQSGALETMLDRVAIYK
EKSELLKQKIKKAMKYPATVIVVAVIVTIILMVKVVPVFEELFTSFGADLPAFTKMVVNMSQWTQKYWFVLIIVVGFAIA
AFLEAKKRSKKFRDLLDKAALRAPIFGDLVYKAIIARYSRTLATTFAAGVPLIDALESTAGATNNVVYEEAVMKIREDVA
TGQQLQFAMRVTDKFPSMAVQMVAIGEESGALDAMLDKVATHYENEVDNAVDGLTSMMEPLIMAVLGILVGGLVVAMYLP
IFQMGSVV

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=1106962 ACLKM4_RS01500 WP_016659656.1 319803..321029(-) (pilC) [Acinetobacter indicus strain D1_NDM_tet(X3)]
ATGGCTGTCAAAAAAGCACAGATGATGCCCACCTTTATTTATGAAGGGATTGACCGCAAAGGGGCAAAACTTAAAGGGGA
ACTGCCAGCACGTAATATGGCGCTGGCCAAGGTCACGTTGCGCAAGCAAGGGATCACCATCAAAACCATCCGCCAGAAGA
AGAAAAATATTCTTGAAGGCTTGATGAAGAAAAAAGTCTCGACCCTGGACATCACGATTTTTACCCGCCAGCTGGCGACC
ATGATGAAAGCCGGTGTACCGCTGGTGCAAAGCTTTGAAATTGTGGCTGAAGGTCTGGAAAACCCGGCCATGCGTGAAGT
GGTATTGGGGATTAAAGGCGAAGTCGAAGGGGGCAATACCTTTGCTGGTGCACTGCGTAAATATCCGCAACATTTCGATA
ATCTGTTTTGCTCGCTGGTTGAATCGGGTGAACAGTCGGGTGCTCTAGAAACTATGCTGGACCGGGTAGCGATCTATAAA
GAAAAGAGCGAATTACTCAAACAAAAAATCAAGAAAGCCATGAAATACCCGGCAACGGTGATTGTGGTGGCGGTGATTGT
CACCATTATCCTGATGGTCAAAGTGGTACCGGTGTTTGAAGAACTCTTCACCTCTTTTGGTGCCGACTTACCAGCCTTCA
CCAAAATGGTGGTGAATATGTCGCAGTGGACCCAGAAATACTGGTTTGTGCTGATTATTGTGGTCGGCTTTGCGATTGCA
GCTTTTCTGGAAGCCAAGAAACGCAGCAAGAAATTCCGTGACCTGCTGGACAAAGCCGCCCTGAGAGCACCAATTTTTGG
TGATCTGGTGTATAAGGCGATTATCGCGCGTTATAGTCGTACCCTGGCCACCACCTTTGCTGCCGGTGTTCCCTTAATTG
ATGCACTGGAATCCACTGCCGGCGCCACCAACAACGTGGTTTATGAAGAAGCAGTAATGAAAATCCGTGAAGATGTCGCC
ACCGGTCAGCAGTTACAGTTTGCCATGCGGGTCACGGATAAATTCCCTTCGATGGCCGTGCAGATGGTTGCGATTGGTGA
AGAATCCGGTGCGCTGGATGCGATGCTGGATAAAGTCGCTACTCACTACGAAAACGAAGTCGATAACGCCGTCGATGGCT
TAACCTCGATGATGGAACCACTGATTATGGCCGTTCTCGGGATTCTGGTCGGCGGTCTGGTGGTCGCGATGTATCTCCCA
ATCTTCCAAATGGGCTCTGTGGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(74-197)

Type II secretion system (T2SS), protein F (T2SSF)

(279-400)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V2UF65

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Acinetobacter baumannii D1279779

88.48

100

0.885
  pilC Acinetobacter baylyi ADP1

84.069

100

0.841
  pilC Pseudomonas stutzeri DSM 10701

60.688

99.755

0.605
  pilC Legionella pneumophila strain ERS1305867

52.593

99.265

0.522
  pilG Neisseria gonorrhoeae MS11

47.99

97.549

0.468
  pilG Neisseria meningitidis 44/76-A

47.99

97.549

0.468
  pilC Vibrio cholerae strain A1552

43.137

100

0.431
  pilC Vibrio campbellii strain DS40M4

42.822

99.02

0.424
  pilC Thermus thermophilus HB27

36.658

98.284

0.36

Multiple sequence alignment