Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACLKM5_RS12800 Genome accession   NZ_CP183893
Coordinates   2670715..2672853 (-) Length   712 a.a.
NCBI ID   WP_411687493.1    Uniprot ID   -
Organism   Acinetobacter indicus strain D10_NDM_tet(X3)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2665715..2677853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM5_RS12785 - 2668182..2669018 (-) 837 WP_411687463.1 hypothetical protein -
  ACLKM5_RS12790 aroB 2669031..2670113 (-) 1083 WP_016658450.1 3-dehydroquinate synthase -
  ACLKM5_RS12795 aroK 2670136..2670678 (-) 543 WP_075167858.1 shikimate kinase AroK -
  ACLKM5_RS12800 pilQ 2670715..2672853 (-) 2139 WP_411687493.1 type IV pilus secretin PilQ Machinery gene
  ACLKM5_RS12805 pilP 2672914..2673441 (-) 528 WP_045795346.1 pilus assembly protein PilP Machinery gene
  ACLKM5_RS12810 pilO 2673441..2674160 (-) 720 WP_411687462.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACLKM5_RS12815 pilN 2674157..2674792 (-) 636 WP_005180516.1 PilN domain-containing protein Machinery gene
  ACLKM5_RS12820 pilM 2674792..2675850 (-) 1059 WP_411687461.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 712 a.a.        Molecular weight: 76873.04 Da        Isoelectric Point: 4.9887

>NTDB_id=1106946 ACLKM5_RS12800 WP_411687493.1 2670715..2672853(-) (pilQ) [Acinetobacter indicus strain D10_NDM_tet(X3)]
MNNLFRQFSMGAVAMAVMQVANAQVSITNIVPMQIPGQGTEIRVMFNGLPPQPAAYQLEQPSRLVLDFDKAQQTLKQASI
PVATREASSVDVSSDAQRARLTVNLAEAGAFTTRVEGNTFILKINSTQAAVTPVTVQQTAQGVSNIGFQRGAEGEGQVVI
DLAGSNTPVDVQQQGSKIIVRTLGNKIPAHLARRLNVNDFATPVSTIDAQNDGGNGIITIQSAGSYEYMAYQAENKLTIS
LKRPVDKNPFKPRAAQSYTGKKISLDFQDIEVRRVLQLLADFTGINMVAADTVQGNITLRLKDVPWDQALDIILKTKNLD
KRRNGNVIWIAPVAELIKAEEEEAKAIAQSIKLAPLQTEYIQLNYAKAADIEKLITDGKNASNSRGGTNSGSGADPLGDS
VGSLLSPRGTVSVDPRTNTLIINDTAEKIDQIRGMIDLLDVSVKQVMIEARIVRATTDFTKEMGVKWGILSQGIMNNNHL
LVGGSDTTLWNLREPEFDDERGGYTYEIERPQNLNVDLGVTSPGASRIAFGLISLSDFMLDLELSALQADGYGEVISTPK
VLTADKQQAKVASGTQIPYQSAEGGGANAVSTTEFIDATLSLDVTPSITPDGKVQMNLNITSDSPGAPTPTGQLTINKNS
INTNVLVDNGETVVLGGIFEQQTANQQTKVPFLGDIPYLGRLFRKDIKSDNKRELLIFVTPRIVNDSVSRNH

Nucleotide


Download         Length: 2139 bp        

>NTDB_id=1106946 ACLKM5_RS12800 WP_411687493.1 2670715..2672853(-) (pilQ) [Acinetobacter indicus strain D10_NDM_tet(X3)]
ATGAACAATTTGTTCCGTCAGTTTTCAATGGGCGCTGTTGCGATGGCCGTCATGCAAGTGGCCAATGCGCAGGTCAGCAT
TACCAATATTGTACCAATGCAAATTCCAGGACAAGGTACTGAAATTCGTGTCATGTTCAATGGCTTGCCACCTCAGCCTG
CAGCCTATCAGCTTGAGCAGCCATCACGTTTGGTGCTGGATTTTGATAAAGCCCAGCAGACCTTAAAACAGGCCAGCATT
CCGGTTGCGACTCGGGAAGCCAGCTCGGTTGATGTCAGTTCCGATGCCCAGCGTGCACGTTTAACGGTTAATCTGGCTGA
AGCGGGTGCTTTTACTACCCGTGTTGAAGGCAATACTTTTATTCTTAAAATTAACAGCACTCAGGCAGCCGTAACGCCAG
TGACGGTACAGCAGACGGCTCAAGGTGTATCGAATATCGGCTTTCAGCGCGGTGCAGAAGGGGAAGGTCAGGTAGTAATT
GACCTGGCAGGCAGCAATACCCCGGTCGATGTACAACAGCAAGGCAGTAAAATTATTGTCCGTACCTTGGGTAATAAAAT
TCCTGCACATCTGGCACGCCGTTTAAACGTCAATGATTTTGCTACGCCGGTATCTACCATTGATGCACAAAATGATGGTG
GCAATGGGATAATTACTATTCAGTCTGCGGGAAGCTATGAATACATGGCGTACCAGGCAGAAAATAAACTGACCATCAGT
CTGAAACGTCCGGTAGATAAGAACCCGTTCAAACCGCGTGCAGCACAAAGCTATACCGGCAAAAAGATTTCTTTAGATTT
CCAGGATATTGAAGTGCGTCGTGTGCTGCAGTTACTGGCTGATTTTACCGGAATTAACATGGTTGCCGCCGATACGGTAC
AAGGCAATATCACCTTACGTCTCAAAGATGTGCCGTGGGATCAGGCACTAGATATTATTTTAAAAACCAAAAATCTGGAT
AAACGCCGTAATGGCAATGTGATCTGGATTGCACCCGTCGCAGAGCTGATCAAGGCCGAAGAAGAAGAAGCTAAAGCGAT
TGCACAAAGTATTAAACTGGCACCGCTGCAAACCGAATATATCCAATTGAATTATGCCAAGGCTGCTGATATCGAGAAAC
TGATTACTGATGGTAAAAACGCATCCAATTCTCGAGGCGGCACAAATTCGGGTTCTGGGGCAGATCCACTTGGTGATAGC
GTCGGTAGTCTGCTCAGCCCACGTGGTACGGTGTCGGTTGATCCACGAACCAACACCCTGATTATTAATGATACTGCAGA
AAAAATTGACCAGATTCGCGGCATGATTGACTTGCTGGATGTATCGGTGAAACAGGTGATGATTGAAGCGCGTATTGTGC
GTGCCACCACTGACTTCACCAAAGAGATGGGGGTCAAGTGGGGCATCCTGTCACAAGGGATCATGAATAATAACCATCTT
CTGGTCGGTGGTAGTGATACCACACTGTGGAATTTACGTGAGCCGGAATTTGACGATGAGCGTGGTGGTTATACCTATGA
AATTGAACGTCCACAAAACTTGAATGTCGATTTGGGGGTCACCAGCCCAGGTGCGAGCCGGATTGCTTTTGGTCTTATCA
GCCTATCTGACTTTATGCTGGATTTAGAACTGTCTGCCTTACAAGCTGATGGCTATGGTGAAGTAATTTCGACCCCGAAA
GTGCTGACTGCTGATAAGCAGCAGGCCAAAGTGGCTTCAGGTACGCAGATTCCATATCAGTCTGCTGAAGGAGGCGGTGC
CAATGCAGTGTCCACCACCGAATTTATTGATGCAACTTTAAGTCTGGATGTTACGCCAAGTATTACCCCGGATGGTAAAG
TACAGATGAACCTGAATATCACCAGTGATAGTCCGGGAGCACCAACGCCGACCGGTCAGCTCACCATTAACAAAAACTCG
ATCAATACCAATGTACTGGTCGATAATGGTGAAACCGTCGTACTCGGTGGTATTTTTGAGCAGCAGACAGCCAATCAGCA
AACCAAAGTGCCATTCCTGGGGGATATTCCGTATTTAGGCCGTTTATTCCGTAAAGATATTAAATCGGACAATAAACGTG
AGTTGCTGATTTTTGTGACACCACGAATTGTTAATGACAGTGTTTCAAGAAATCATTAA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(40-121)

Secretin and TonB N terminus short domain (STN)

(290-332)

Bacterial type II/III secretion system short domain (Secretin_N)

(359-445)

Bacterial type II and III secretion system protein (Secretin)

(546-704)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Acinetobacter baumannii D1279779

76.593

100

0.777
  pilQ Acinetobacter baumannii strain A118

76.454

100

0.775
  comQ Acinetobacter baylyi ADP1

74.09

100

0.743
  pilQ Pseudomonas aeruginosa PAK

40.371

98.455

0.397
  pilQ Legionella pneumophila strain Lp02

38.601

100

0.388
  pilQ Legionella pneumophila strain ERS1305867

38.601

100

0.388

Multiple sequence alignment