Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ACLKM5_RS12810 Genome accession   NZ_CP183893
Coordinates   2673441..2674160 (-) Length   239 a.a.
NCBI ID   WP_411687462.1    Uniprot ID   -
Organism   Acinetobacter indicus strain D10_NDM_tet(X3)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2668441..2679160
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM5_RS12790 aroB 2669031..2670113 (-) 1083 WP_016658450.1 3-dehydroquinate synthase -
  ACLKM5_RS12795 aroK 2670136..2670678 (-) 543 WP_075167858.1 shikimate kinase AroK -
  ACLKM5_RS12800 pilQ 2670715..2672853 (-) 2139 WP_411687493.1 type IV pilus secretin PilQ Machinery gene
  ACLKM5_RS12805 pilP 2672914..2673441 (-) 528 WP_045795346.1 pilus assembly protein PilP Machinery gene
  ACLKM5_RS12810 pilO 2673441..2674160 (-) 720 WP_411687462.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACLKM5_RS12815 pilN 2674157..2674792 (-) 636 WP_005180516.1 PilN domain-containing protein Machinery gene
  ACLKM5_RS12820 pilM 2674792..2675850 (-) 1059 WP_411687461.1 pilus assembly protein PilM Machinery gene
  ACLKM5_RS12825 ponA 2676022..2678598 (+) 2577 WP_411687460.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 239 a.a.        Molecular weight: 27117.73 Da        Isoelectric Point: 5.1311

>NTDB_id=1106948 ACLKM5_RS12810 WP_411687462.1 2673441..2674160(-) (pilO) [Acinetobacter indicus strain D10_NDM_tet(X3)]
MSLDQYEEMNQDPVAAPKKKMTLDKFLQQWNTLDPNNYGSWPFSVKLTIWIFIIILIAFLGYFLAIKPKLDAISNARAQE
QNLLNEFREKDSKLRNLQQYQAQLQEMEANFNQQLQQLPKETEIPGLVEDVNVTGVNSGLRFKNIRLENEIKQEFFIEQP
ISIEATGDYHAFGSFVSGTAALPRIVTMHDFTITAGENKEKKTDIPQITYVAKAKTYRYVGNTDAQAGNSTTPTTGAAQ

Nucleotide


Download         Length: 720 bp        

>NTDB_id=1106948 ACLKM5_RS12810 WP_411687462.1 2673441..2674160(-) (pilO) [Acinetobacter indicus strain D10_NDM_tet(X3)]
ATGAGTCTCGATCAATATGAAGAAATGAATCAGGATCCTGTTGCTGCGCCAAAGAAAAAAATGACGCTGGATAAATTCCT
GCAGCAATGGAATACCTTAGATCCGAATAACTATGGCAGCTGGCCGTTTTCCGTCAAGCTGACCATCTGGATCTTTATCA
TTATTTTAATTGCCTTCCTGGGCTATTTCCTGGCCATCAAGCCGAAGCTGGATGCAATTTCCAATGCGCGTGCACAAGAG
CAGAACCTGCTGAATGAGTTTCGTGAAAAAGATTCCAAACTGCGTAACTTGCAGCAATATCAGGCGCAGTTACAGGAAAT
GGAAGCCAACTTTAACCAGCAGCTACAACAGTTGCCAAAAGAAACCGAAATTCCAGGTCTGGTTGAAGATGTCAACGTTA
CGGGCGTGAATTCAGGCTTAAGATTTAAAAATATCCGTCTGGAAAATGAAATCAAGCAAGAGTTCTTTATCGAGCAGCCA
ATTTCGATTGAAGCCACCGGTGATTATCATGCTTTTGGTTCTTTTGTGAGCGGTACAGCGGCTTTACCGCGAATTGTGAC
CATGCATGATTTCACTATTACTGCCGGTGAAAACAAGGAAAAGAAAACCGATATTCCACAAATTACCTATGTCGCGAAAG
CCAAGACCTATCGCTATGTCGGGAATACCGATGCACAGGCCGGGAATAGCACGACTCCAACCACAGGAGCAGCACAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(74-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

69.328

99.582

0.69
  comO Acinetobacter baylyi ADP1

67.355

100

0.682

Multiple sequence alignment