Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACLKM6_RS01275 Genome accession   NZ_CP183890
Coordinates   274083..275789 (-) Length   568 a.a.
NCBI ID   WP_104471790.1    Uniprot ID   -
Organism   Acinetobacter indicus strain D11_NDM_tet(X5)     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 269083..280789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM6_RS01245 - 269201..269524 (+) 324 WP_005181767.1 pyrimidine/purine nucleoside phosphorylase -
  ACLKM6_RS01250 rlmB 269630..270382 (+) 753 WP_411687059.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACLKM6_RS01255 - 270400..271320 (+) 921 WP_016659657.1 DMT family transporter -
  ACLKM6_RS01260 coaE 271317..271925 (-) 609 WP_411687058.1 dephospho-CoA kinase -
  ACLKM6_RS01265 pilD 271936..272799 (-) 864 WP_411687057.1 prepilin peptidase Machinery gene
  ACLKM6_RS01270 pilC 272823..274052 (-) 1230 WP_411687056.1 type II secretion system F family protein Machinery gene
  ACLKM6_RS01275 pilB 274083..275789 (-) 1707 WP_104471790.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLKM6_RS01280 tpiA 276078..276869 (+) 792 WP_005181780.1 triose-phosphate isomerase -
  ACLKM6_RS01285 secG 276883..277212 (+) 330 WP_005181783.1 preprotein translocase subunit SecG -
  ACLKM6_RS01310 rimP 278081..278596 (+) 516 WP_411687055.1 ribosome maturation factor RimP -
  ACLKM6_RS01315 nusA 278629..280113 (+) 1485 WP_005181786.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63759.15 Da        Isoelectric Point: 4.8301

>NTDB_id=1106880 ACLKM6_RS01275 WP_104471790.1 274083..275789(-) (pilB) [Acinetobacter indicus strain D11_NDM_tet(X5)]
MSVQQAMPRFTGFIRRLVEEGLVTAENMQQAIVAAKKAETDIVPYLIQQLQISPLTIAEKISYEFGEPIFDLAVYDSRLI
VREGLDEKLFTKYRILPIFRRHNQLYIATSNPTNIEAIDAIRFNSKLNIEVVIVEHDKLERLIEQNFTEDSTFDFDEDFD
LEVGDNAPETQEDDDEPQGDEAPIVKYINKLLVDAIRMGASDLHFEPYEKSYRVRYRIDGVLRQIANPPLQLATRLASRL
KVMSQMDISEKRIPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPEQKELFMEALDKPQG
MLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIVMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCQKCKAPVEIPRQSLLELGFTE
EDLNQPEFQLYQPVGCPECREGYKGRVGVYEVMKVTPEISRIIMEDGNALQIAAASAKSGFNNLRRSGLIKVMQGVTSLQ
EVNRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=1106880 ACLKM6_RS01275 WP_104471790.1 274083..275789(-) (pilB) [Acinetobacter indicus strain D11_NDM_tet(X5)]
ATGTCAGTACAACAAGCAATGCCTAGATTTACAGGTTTTATTCGGCGACTGGTCGAAGAAGGGCTTGTGACGGCTGAAAA
TATGCAGCAAGCCATTGTCGCGGCGAAAAAAGCCGAGACCGATATTGTGCCGTATTTAATTCAGCAGTTGCAGATTTCAC
CGCTGACCATTGCTGAAAAAATTTCCTATGAATTTGGTGAACCGATTTTTGACTTGGCGGTGTACGATTCGCGTCTGATT
GTACGTGAAGGTCTGGATGAAAAACTGTTCACCAAATACCGAATTCTGCCCATTTTCCGACGTCACAATCAGCTTTATAT
TGCTACCAGTAACCCAACCAATATCGAAGCCATTGATGCGATCCGCTTTAACAGCAAGCTGAATATTGAAGTGGTGATTG
TTGAGCATGACAAGCTGGAACGGCTGATTGAACAGAACTTTACCGAAGACAGCACCTTTGACTTTGATGAAGACTTTGAT
CTGGAAGTTGGCGACAACGCTCCCGAAACACAAGAAGATGACGATGAGCCACAAGGCGATGAAGCCCCAATCGTCAAATA
TATTAACAAGTTACTGGTCGATGCAATCCGTATGGGCGCCTCGGATTTACACTTCGAGCCTTATGAAAAAAGCTACCGGG
TCCGTTACCGGATTGACGGCGTTTTGCGCCAGATTGCCAATCCTCCGTTACAACTGGCCACACGCCTTGCCTCACGTTTA
AAAGTCATGTCACAAATGGACATCTCGGAAAAACGTATTCCGCAGGATGGCCGGATCAAGCTCAAGCTGTCAAAAAACAA
AGCCATCGACTTCCGTGTCAACTCATTGCCGACCCTGTTTGGAGAAAAAATCGTACTGCGTATCCTCGATCCATCTAGCG
CCATGCTGGGGATTGATGCACTCGGTTATGAACCGGAGCAAAAAGAGCTGTTTATGGAGGCGCTGGACAAACCACAAGGC
ATGTTGCTGATTACCGGTCCGACCGGTTCTGGTAAAACCGTGTCTTTATATACCGGCTTAAATATCCTCAACCGCGAAGA
CACCAATATTTCTACCGCCGAAGATCCGGTAGAAATTAACCTGGAAGGCATCAATCAGGTCAACGTCAACCCCAAAGTGG
GTCTGACCTTTGCCGCAGCACTAAAATCATTCCTGCGTCAGGATCCGGATATTGTCATGGTCGGTGAGATCCGTGACCTG
GAAACCGCAGAAATTGCCATTAAAGCCGCACAAACCGGTCACATGGTCATGTCGACGCTGCACACCAACAGTGCACCCGA
AACCCTGACCCGTTTACGCAACATGGGCGTCCCCTCATTTAACATCGCCACCTCGGTGAACCTGGTGATTGCCCAGCGTC
TGGCACGTCGTCTCTGCCAGAAATGCAAGGCCCCGGTGGAGATTCCACGCCAGAGCCTCTTGGAACTCGGTTTTACCGAA
GAAGATTTAAACCAGCCAGAGTTCCAGCTGTATCAGCCGGTTGGCTGCCCGGAATGTCGTGAAGGTTATAAAGGCCGTGT
CGGGGTCTATGAAGTAATGAAAGTCACCCCGGAAATCTCACGCATTATTATGGAAGATGGTAATGCCTTGCAAATTGCAG
CAGCCTCGGCAAAATCCGGGTTTAACAATTTACGCCGTTCAGGATTAATCAAGGTCATGCAGGGGGTCACTTCTTTACAG
GAAGTGAATCGTGTGACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.526

100

0.808
  pilB Acinetobacter baylyi ADP1

78.07

100

0.783
  pilB Legionella pneumophila strain ERS1305867

50.97

99.824

0.509
  pilF Neisseria gonorrhoeae MS11

51.429

98.592

0.507
  pilB Vibrio cholerae strain A1552

49.647

99.648

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

49.551

98.063

0.486
  pilB Vibrio campbellii strain DS40M4

50.864

91.725

0.467
  pilF Thermus thermophilus HB27

37.258

100

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

33.821

100

0.366

Multiple sequence alignment