Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACAM27_RS08365 Genome accession   NZ_AP031605
Coordinates   1854537..1856255 (-) Length   572 a.a.
NCBI ID   WP_369612824.1    Uniprot ID   -
Organism   Marinobacter sp. RI1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1849537..1861255
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM27_RS08340 (MspRI1_16640) tsaA 1849918..1850667 (-) 750 WP_153741130.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  ACAM27_RS08345 (MspRI1_16650) - 1850676..1851665 (-) 990 WP_369612821.1 hypothetical protein -
  ACAM27_RS08350 (MspRI1_16660) coaE 1851754..1852371 (-) 618 WP_369612822.1 dephospho-CoA kinase -
  ACAM27_RS08355 (MspRI1_16670) pilD 1852372..1853247 (-) 876 WP_369612823.1 A24 family peptidase Machinery gene
  ACAM27_RS08360 (MspRI1_16680) pilC 1853314..1854534 (-) 1221 WP_222179186.1 type II secretion system F family protein Machinery gene
  ACAM27_RS08365 (MspRI1_16690) pilB 1854537..1856255 (-) 1719 WP_369612824.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACAM27_RS08370 (MspRI1_16700) - 1856642..1857127 (+) 486 WP_369612825.1 pilin -
  ACAM27_RS08375 - 1857203..1859113 (+) 1911 WP_369612826.1 hypothetical protein -
  ACAM27_RS08380 (MspRI1_16720) - 1859230..1859709 (-) 480 WP_369612827.1 pilin -
  ACAM27_RS08385 (MspRI1_16730) - 1859899..1860540 (-) 642 WP_369612828.1 HAD-IB family hydrolase -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62816.80 Da        Isoelectric Point: 4.8542

>NTDB_id=110609 ACAM27_RS08365 WP_369612824.1 1854537..1856255(-) (pilB) [Marinobacter sp. RI1]
MATSNRNVTLTGLARRFVDDGLLDEETAKDAFIQASQNRIPLITYLTQNQLADSSRLAFSAAMEFGVSVLDLDSFLPEMM
PEKVVDEKLVRKHNALPLYKRGNRLFIAVSDPTNIQALDEIKFNTGLSTDAILVDDSKLRAAIDKYLESQDTTMGDLDDA
DLEGVETESGEGEDDDGAVSATDIDDAPIVKYVNKMLLDAIRGGASDIHFEPYEKVYRVRYRTDGILKEVSRPSIKLAPK
ISARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDVLGYEEDQKQLYMDAL
AQPQGMILVTGPTGSGKTVSLYTGLNILNTPGINISTAEDPAEINLEGINQVNVNNRVGLGFAEALRAFLRQDPDVIMVG
EIRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIATSVSLIIAQRLGRRLCSSCKQPADVPKDVLLK
EGFTQEQIDTGFTLYRPKGCDKCNGGYKGRVGIYEVVKITDELASMIMEEASSIKIAKQAQAEGFRNLRQSALLKVIEGV
TSLEEANRVTKD

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=110609 ACAM27_RS08365 WP_369612824.1 1854537..1856255(-) (pilB) [Marinobacter sp. RI1]
ATGGCGACCAGCAACCGAAACGTGACCTTGACGGGCCTTGCCCGCCGCTTTGTAGATGACGGCCTTCTGGACGAGGAAAC
CGCAAAAGACGCATTCATCCAAGCGTCCCAGAACCGAATTCCACTGATTACTTATTTGACTCAAAATCAACTTGCCGACA
GTTCCAGGCTTGCCTTTTCTGCCGCGATGGAATTCGGTGTTTCGGTACTGGATCTGGATTCATTCCTCCCGGAAATGATG
CCGGAGAAGGTAGTCGACGAAAAGTTGGTTCGAAAGCATAACGCCCTCCCTCTTTACAAAAGGGGCAATCGGCTTTTCAT
TGCGGTTTCTGACCCCACCAATATCCAAGCTTTGGATGAGATTAAATTCAATACTGGCCTGAGTACAGATGCCATTCTAG
TTGATGACTCAAAGCTACGCGCGGCTATTGATAAGTACCTGGAATCCCAGGACACGACAATGGGTGATCTGGACGATGCC
GATCTGGAGGGCGTTGAAACAGAAAGCGGTGAAGGCGAGGATGACGATGGCGCCGTATCTGCTACAGACATTGACGATGC
GCCGATTGTTAAATATGTAAACAAAATGCTTTTGGATGCCATTCGTGGTGGTGCGTCAGATATTCATTTTGAACCTTACG
AAAAAGTTTATCGTGTGCGCTACCGTACCGACGGCATATTGAAAGAGGTTTCACGGCCTTCTATTAAATTGGCACCAAAG
ATCTCGGCCCGGGTAAAGATCATGGCTCAGCTAGATATTTCTGAGCGCAGGGTACCGCAGGACGGCCGGATCAAAATGAA
GCTGTCCAAAACCAAAGCGATCGATTTCCGTGTAAACACCCTGCCAACCCTTTGGGGCGAAAAAATCGTTCTTCGGATTC
TTGATCCCAGTCAGGCAAAACTTGGCATTGACGTGCTCGGATACGAAGAAGACCAGAAACAGCTTTATATGGATGCCCTG
GCCCAGCCTCAAGGTATGATTCTGGTTACAGGGCCGACTGGTTCAGGTAAGACCGTCTCGCTTTACACCGGCCTTAACAT
TCTGAACACCCCAGGTATCAATATATCGACGGCAGAAGATCCGGCAGAGATCAACCTGGAAGGCATCAATCAGGTAAACG
TAAACAACCGTGTAGGACTTGGATTTGCCGAGGCACTGCGCGCCTTCCTCCGGCAAGATCCGGACGTGATCATGGTGGGT
GAGATCCGGGATCTGGAAACTGCCAACATCGCAATCAAAGCGGCGCAGACCGGACACCTGGTTCTTTCCACGCTGCACAC
GAACAGCGCAGCCGAGACACTCACCCGTATGATGAACATGGGGGTGCCCGCTTTTAACATAGCCACATCGGTCAGCCTGA
TCATTGCCCAGCGCTTGGGTCGAAGGCTCTGCAGTAGCTGCAAACAACCCGCCGATGTGCCTAAAGACGTGCTTTTGAAG
GAAGGGTTCACACAAGAACAAATTGATACAGGCTTCACGTTGTACCGCCCTAAGGGCTGTGATAAATGCAATGGTGGATA
TAAAGGCCGCGTTGGTATTTACGAGGTGGTCAAGATTACCGACGAGCTGGCAAGTATGATTATGGAAGAGGCCAGCTCCA
TCAAAATTGCAAAGCAGGCTCAGGCGGAAGGCTTCCGAAACCTCAGGCAATCGGCACTTCTCAAGGTTATTGAGGGCGTG
ACCAGCCTTGAAGAAGCCAACCGTGTGACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.846

100

0.573
  pilB Acinetobacter baylyi ADP1

56.738

98.601

0.559
  pilB Legionella pneumophila strain ERS1305867

53.993

100

0.544
  pilB Vibrio parahaemolyticus RIMD 2210633

51.892

97.028

0.503
  pilB Vibrio campbellii strain DS40M4

50.442

98.776

0.498
  pilB Vibrio cholerae strain A1552

55.253

89.86

0.497
  pilF Neisseria gonorrhoeae MS11

47.972

99.126

0.476
  pilB/pilB1 Synechocystis sp. PCC 6803

37.4

100

0.407
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.848

92.133

0.367
  pilF Thermus thermophilus HB27

37.365

96.853

0.362

Multiple sequence alignment