Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACNYC6_RS17950 Genome accession   NZ_CP183416
Coordinates   3948703..3950403 (-) Length   566 a.a.
NCBI ID   WP_419708525.1    Uniprot ID   -
Organism   Pseudomonas sp. NFX224     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3943703..3955403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNYC6_RS17920 (ACNYC6_17920) - 3944637..3944879 (-) 243 WP_419708520.1 DUF2790 domain-containing protein -
  ACNYC6_RS17925 (ACNYC6_17925) - 3945067..3945756 (+) 690 WP_419708521.1 energy-coupling factor ABC transporter permease -
  ACNYC6_RS17930 (ACNYC6_17930) yacG 3945792..3945992 (-) 201 WP_034148252.1 DNA gyrase inhibitor YacG -
  ACNYC6_RS17935 (ACNYC6_17935) coaE 3945989..3946612 (-) 624 WP_419708522.1 dephospho-CoA kinase -
  ACNYC6_RS17940 (ACNYC6_17940) pilD 3946609..3947481 (-) 873 WP_419708523.1 prepilin peptidase Machinery gene
  ACNYC6_RS17945 (ACNYC6_17945) pilC 3947483..3948700 (-) 1218 WP_419708524.1 type II secretion system F family protein Machinery gene
  ACNYC6_RS17950 (ACNYC6_17950) pilB 3948703..3950403 (-) 1701 WP_419708525.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACNYC6_RS17955 (ACNYC6_17955) pilA 3950629..3951048 (+) 420 WP_419708526.1 pilin Machinery gene
  ACNYC6_RS17960 (ACNYC6_17960) - 3951358..3951714 (+) 357 WP_419708527.1 BON domain-containing protein -
  ACNYC6_RS17965 (ACNYC6_17965) - 3951874..3952176 (-) 303 WP_419708528.1 DUF2845 domain-containing protein -
  ACNYC6_RS17970 (ACNYC6_17970) - 3952176..3955127 (-) 2952 WP_419708529.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62791.91 Da        Isoelectric Point: 5.8304

>NTDB_id=1105664 ACNYC6_RS17950 WP_419708525.1 3948703..3950403(-) (pilB) [Pseudomonas sp. NFX224]
MNDIALSGLAKQLVLAELLTDKSAQQASQQAQRNRISLVSYLVQNKLVKSRQVAEIASEHFGMALLDLHALDKEIQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSEAIEKFFDSHGTGLEDMADVDLDG
LDIETADDKRQDAIVGQDADDAPVVRFVHKMLLDAIRSGSSDLHFEPYEKIYRVRMRTDGMLREVAKPPIQLANRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPEQQDLYMAALRQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGMDFAQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKASQTGHLVLSTLHTNSAAQTLTRLQNMGIQGFNIATSVSLIIAQRLARKLCSHCRVPLDIPREALLKEGFPE
ERIGTFTIYEPVGCEHCNGGYKGRVGIYEVVKNTADLQRLIMAEGNSLEIEEQMRKDGFNDLRTSGLLKAMQGITSLEEI
NRVSKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1105664 ACNYC6_RS17950 WP_419708525.1 3948703..3950403(-) (pilB) [Pseudomonas sp. NFX224]
ATGAATGACATCGCTCTCAGCGGTCTGGCCAAGCAATTGGTCCTGGCCGAGCTGCTCACTGACAAAAGTGCACAACAGGC
GTCCCAGCAAGCCCAGCGCAACCGCATCTCCCTGGTCAGCTATCTGGTGCAGAACAAACTGGTCAAAAGCCGCCAGGTCG
CCGAGATCGCCTCGGAACACTTCGGCATGGCCCTGTTGGACCTCCATGCCCTGGACAAGGAGATTCAGCCCAAGGGCCTC
GTCAGCGAGAAATTGGTCCGCCAGCACCATGCGCTGCCCCTGTGGCGCCGAGGCAACAAACTGTTTGTGGGGATTTCCGA
CCCGACCAATCACCAGGCCATCAACGACATCCAGTTCAGCACCGGGCTTACCACCGAAGCGATTCTGGTCGAGGACGACA
AGCTCAGCGAGGCCATCGAGAAATTCTTCGACAGCCATGGCACCGGCCTGGAAGACATGGCCGACGTTGACCTCGACGGC
CTGGACATCGAGACCGCCGATGACAAACGTCAGGACGCCATCGTCGGCCAGGATGCCGATGACGCGCCGGTGGTGCGCTT
CGTCCATAAAATGTTGCTCGACGCGATAAGAAGCGGCTCTTCCGACCTGCATTTCGAGCCTTACGAGAAAATCTATCGCG
TGCGGATGCGTACCGACGGCATGCTGCGGGAAGTCGCCAAGCCACCGATCCAGTTGGCCAACCGTATCGCCGCGCGGCTG
AAGGTCATGGCCAGCCTGGACATCTCGGAACGCCGCAAACCCCAGGACGGGCGGATCAAGATGCGCTTGTCCAAAAGCAA
ATCCATCGACTTCAGGGTCAACACCTTGCCGACCCTGTGGGGCGAGAAAGTGGTGATCCGGATTCTTGATCCCTCCAGCG
CGCAAATGGGCATCGACGCCCTCGGCTACGAGCCTGAACAGCAAGACCTGTACATGGCCGCGCTCAGGCAGCCACAGGGC
ATGATTCTGGTGACCGGCCCTACCGGCTCGGGCAAAACCGTGTCGCTCTACACCGGGCTGAACATCCTCAACACCGTGGA
CATCAACATTTCCACCGCTGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCAAAGCAGG
GGATGGATTTCGCTCAGGCGCTGCGCGCGTTCCTGCGCCAGGACCCAGACGTGATCATGGTCGGTGAAATCCGCGATCTG
GAAACCGCCGAAATCGCCATCAAGGCTTCACAGACCGGGCACCTGGTGCTGTCGACCCTGCACACCAACAGTGCCGCCCA
AACCCTGACCCGCTTGCAGAACATGGGCATCCAGGGCTTCAACATCGCCACCTCCGTCAGCCTGATCATCGCCCAGCGCC
TGGCGCGCAAGCTGTGCAGCCATTGCCGGGTGCCGCTCGACATTCCTCGCGAGGCGCTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCACATTCACGATCTATGAGCCGGTCGGCTGCGAACACTGCAACGGCGGCTACAAAGGGCGAGTGGGGAT
TTATGAAGTGGTGAAGAACACAGCGGACTTGCAACGATTGATCATGGCCGAAGGCAACTCACTTGAAATCGAAGAGCAGA
TGCGCAAGGACGGCTTCAATGACCTGCGCACCTCGGGCCTGCTCAAAGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTTTCCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.979

99.647

0.578
  pilB Acinetobacter baumannii D1279779

57.447

99.647

0.572
  pilB Legionella pneumophila strain ERS1305867

55.124

100

0.551
  pilB Vibrio cholerae strain A1552

50.353

100

0.504
  pilB Vibrio campbellii strain DS40M4

49.291

99.647

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

50.364

97.173

0.489
  pilF Neisseria gonorrhoeae MS11

48.49

99.47

0.482
  pilF Thermus thermophilus HB27

36.572

100

0.366

Multiple sequence alignment