Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACN08S_RS18990 Genome accession   NZ_CP183265
Coordinates   1560912..1562597 (+) Length   561 a.a.
NCBI ID   WP_419208946.1    Uniprot ID   -
Organism   Photobacterium leiognathi subsp. mandapamensis strain Sr2.36     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1555912..1567597
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN08S_RS18965 (ACN08S_19000) ampD 1556686..1557231 (-) 546 WP_419208944.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACN08S_RS18970 (ACN08S_19005) nadC 1557748..1558635 (+) 888 WP_419209682.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACN08S_RS18975 (ACN08S_19010) - 1558983..1559060 (+) 78 Protein_1438 type IV pilin protein -
  ACN08S_RS18980 (ACN08S_19015) - 1559171..1560487 (+) 1317 WP_419208245.1 ISNCY family transposase -
  ACN08S_RS18985 (ACN08S_19020) - 1560510..1560908 (+) 399 WP_419208945.1 pilin -
  ACN08S_RS18990 (ACN08S_19025) pilB 1560912..1562597 (+) 1686 WP_419208946.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACN08S_RS18995 (ACN08S_19030) pilC 1562642..1563865 (+) 1224 WP_419208947.1 type II secretion system F family protein Machinery gene
  ACN08S_RS19000 (ACN08S_19035) pilD 1563954..1564823 (+) 870 WP_419208948.1 prepilin peptidase Machinery gene
  ACN08S_RS19005 (ACN08S_19040) coaE 1564827..1565432 (+) 606 WP_419208949.1 dephospho-CoA kinase -
  ACN08S_RS19010 (ACN08S_19045) zapD 1565497..1566243 (+) 747 WP_419208950.1 cell division protein ZapD -
  ACN08S_RS19015 (ACN08S_19050) yacG 1566251..1566472 (+) 222 WP_318469651.1 DNA gyrase inhibitor YacG -
  ACN08S_RS19020 (ACN08S_19055) mutT 1566477..1566881 (+) 405 WP_419208951.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62137.56 Da        Isoelectric Point: 5.0205

>NTDB_id=1104925 ACN08S_RS18990 WP_419208946.1 1560912..1562597(+) (pilB) [Photobacterium leiognathi subsp. mandapamensis strain Sr2.36]
MQTNLASILAQANLISPEQEQQVVEFVTAEGINVPTALTRLGVLSDLDLADNLANLFGLQKVDVHHYDYDGCCQSVYQRE
LVLRHRALPLTQDANSLFLGVSDPTNMDAQDEFRFAVGKTVEFLILENKQLESAIRRVYGSDIGETSHSKQVSDADLGEL
VDINDAELDEATDLTQDSAPVTRYINQVLLDAVRRKASDIHFEPYEHNYRIRFRCDGILHQHLTPPASLSRRLSTRLKVM
SKLNIAERRQPQDGRIKLKLSDTLAIDLRVSTLPTLWGEKVVLRILDSSSANLDINILGYNDKQKSDYLTALERPQGMIL
ITGPTGSGKTVSLYTGLNILNTDERNISTAEDPVEINLPGINQLQINQQVGLSFADALRSFLRQDPDIVMVGEIRDLETA
SIAVKAAQTGHLVLSTLHTNSAAETITRLTNMGVEDFNLASSLSLIIAQRLARRLCKSCKHPHPLDEITCQRLNVPLNNT
VYQANNAGCDDCNKGYAGRVGIYEVMVFSREIAEALMANATALQLEDIAVKQGMQRLKDSGVEKLLEGVTSLSELQRVLQ
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1104925 ACN08S_RS18990 WP_419208946.1 1560912..1562597(+) (pilB) [Photobacterium leiognathi subsp. mandapamensis strain Sr2.36]
ATGCAAACCAATCTCGCTTCTATCTTGGCTCAAGCTAATCTAATTAGCCCAGAGCAAGAACAACAAGTGGTCGAGTTTGT
AACGGCGGAAGGGATTAACGTCCCGACCGCCTTAACCCGTTTAGGAGTATTAAGCGATTTAGATTTAGCCGATAACCTAG
CTAATTTATTTGGCTTACAAAAAGTCGATGTCCACCATTATGACTATGATGGCTGTTGTCAAAGTGTCTACCAACGTGAA
TTAGTCCTACGCCACCGCGCACTGCCATTAACCCAAGATGCCAACAGTCTATTTTTAGGGGTCAGTGATCCTACCAATAT
GGATGCGCAAGACGAGTTTCGTTTTGCGGTAGGCAAAACCGTTGAGTTTCTAATTTTAGAAAACAAACAGTTAGAATCTG
CCATTCGCCGTGTTTACGGTAGCGATATTGGTGAAACTAGCCACAGCAAACAAGTCAGTGACGCTGATTTAGGTGAACTG
GTTGATATTAACGATGCAGAGCTTGATGAGGCCACCGATTTAACCCAAGATAGCGCCCCTGTAACACGCTATATCAACCA
AGTGTTACTAGATGCCGTACGTCGTAAAGCGTCAGATATTCACTTTGAACCCTACGAGCATAACTACCGTATTCGTTTTC
GCTGTGACGGTATTTTGCATCAACATTTAACTCCACCAGCGAGTTTGTCACGCCGTTTATCGACCCGTTTAAAGGTAATG
TCGAAGCTCAACATTGCTGAGCGCCGCCAACCACAAGATGGACGTATCAAGTTAAAACTATCTGATACCTTAGCAATAGA
TTTGCGTGTCTCTACCCTACCTACACTGTGGGGAGAAAAAGTAGTACTGCGGATATTAGATAGCTCTTCGGCAAATCTTG
ATATCAATATTCTCGGTTACAACGATAAGCAAAAATCCGATTATTTAACCGCCCTTGAACGCCCACAAGGGATGATATTA
ATTACAGGCCCTACAGGTAGTGGTAAAACTGTTTCGCTTTATACCGGGCTAAATATTTTAAATACCGATGAGCGCAATAT
TTCTACTGCTGAAGATCCGGTGGAAATTAACTTACCCGGTATTAACCAATTACAAATCAATCAGCAAGTGGGTTTAAGTT
TTGCCGACGCACTGCGTTCATTTCTACGCCAAGACCCCGACATTGTGATGGTTGGTGAGATCCGTGATTTAGAAACCGCC
TCTATTGCGGTAAAAGCCGCCCAAACAGGTCACTTGGTATTATCAACGCTACATACTAACTCTGCTGCCGAAACCATTAC
CCGTTTAACCAATATGGGAGTGGAAGATTTTAACCTCGCTTCATCGCTGAGCTTAATCATTGCCCAGCGTCTTGCCCGAC
GTTTATGTAAAAGCTGTAAACACCCTCACCCGCTAGATGAAATCACTTGCCAGCGTCTAAACGTGCCGCTCAATAACACG
GTTTATCAAGCCAATAATGCAGGTTGTGATGATTGTAATAAAGGCTATGCCGGACGTGTCGGTATTTATGAAGTAATGGT
ATTTAGCCGCGAAATAGCCGAAGCCTTAATGGCGAATGCCACTGCATTACAACTAGAAGATATTGCTGTTAAACAAGGCA
TGCAGCGCTTAAAAGATTCAGGTGTTGAGAAGTTGCTTGAAGGCGTAACTAGCCTATCTGAACTGCAACGTGTATTACAG
CTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-454)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

62.321

99.822

0.622
  pilB Vibrio campbellii strain DS40M4

59.609

100

0.597
  pilB Vibrio parahaemolyticus RIMD 2210633

59.643

99.822

0.595
  pilB Acinetobacter baumannii D1279779

52.998

92.157

0.488
  pilB Legionella pneumophila strain ERS1305867

48.233

100

0.487
  pilB Acinetobacter baylyi ADP1

51.638

92.513

0.478
  pilF Neisseria gonorrhoeae MS11

46.004

100

0.462
  pilF Thermus thermophilus HB27

38.053

100

0.383
  pilB/pilB1 Synechocystis sp. PCC 6803

40

91.8

0.367

Multiple sequence alignment