Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACNS8M_RS01410 Genome accession   NZ_CP183239
Coordinates   310895..312607 (-) Length   570 a.a.
NCBI ID   WP_168417583.1    Uniprot ID   -
Organism   Acinetobacter sp. A2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 305895..317607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNS8M_RS01380 (ACNS8M_01360) - 305941..306264 (+) 324 WP_168417589.1 pyrimidine/purine nucleoside phosphorylase -
  ACNS8M_RS01385 (ACNS8M_01365) rlmB 306443..307192 (+) 750 WP_168541325.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACNS8M_RS01390 (ACNS8M_01370) - 307234..308163 (+) 930 WP_168417587.1 DMT family transporter -
  ACNS8M_RS01395 (ACNS8M_01375) coaE 308160..308768 (-) 609 WP_168417586.1 dephospho-CoA kinase -
  ACNS8M_RS01400 (ACNS8M_01380) pilD 308778..309638 (-) 861 WP_168541327.1 prepilin peptidase Machinery gene
  ACNS8M_RS01405 (ACNS8M_01385) pilC 309638..310864 (-) 1227 WP_168417584.1 type II secretion system F family protein Machinery gene
  ACNS8M_RS01410 (ACNS8M_01390) pilB 310895..312607 (-) 1713 WP_168417583.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACNS8M_RS01415 (ACNS8M_01395) tpiA 312885..313676 (+) 792 WP_168417582.1 triose-phosphate isomerase -
  ACNS8M_RS01420 (ACNS8M_01400) secG 313690..314019 (+) 330 WP_168417581.1 preprotein translocase subunit SecG -
  ACNS8M_RS01445 (ACNS8M_01425) rimP 315177..315701 (+) 525 WP_168460415.1 ribosome maturation factor RimP -
  ACNS8M_RS01450 (ACNS8M_01430) nusA 315737..317221 (+) 1485 WP_168417579.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63545.81 Da        Isoelectric Point: 4.7343

>NTDB_id=1104731 ACNS8M_RS01410 WP_168417583.1 310895..312607(-) (pilB) [Acinetobacter sp. A2]
MSVQQATPRFTGFIRRLVEDGLVSAENMQTAIASAQKEKIDIVPYLIQNLNISPLLIAEKISNEFGEPIFDLGVYDSALI
VREGIDEKLFSKYCLLPIFKRNNLLYVATSNPTNIEAMDAIRFNSKMNVEAIVVEHDKLAKLIEQNFAGESAFEFEDDDF
ELNFDEGDNEQEQATEDEPQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKTYRVRYRVDGVLRQIANPPLQLANRMAS
RLKVMSQMDISEKRVPQDGRIKLKLSKNKSIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKVGLTFAAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNMVIAQRLARRLCSYCKTPSDIPKQSLLEMGF
TEADLNNPEFQVYQPVGCAECREGYKGRVGIYEVMKVTPEISKLIMEDGNAIQIAEASAKLGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1104731 ACNS8M_RS01410 WP_168417583.1 310895..312607(-) (pilB) [Acinetobacter sp. A2]
ATGTCAGTACAACAAGCTACACCTAGATTCACAGGTTTTATTCGGCGTTTGGTCGAAGATGGGCTCGTTTCTGCTGAAAA
CATGCAAACTGCGATTGCCTCTGCACAAAAAGAAAAAATTGACATTGTTCCCTATCTCATTCAGAACCTGAATATTTCAC
CTTTGCTGATTGCTGAAAAAATTTCCAATGAATTTGGTGAACCTATTTTTGACTTAGGTGTCTATGATTCCGCTTTGATT
GTGCGTGAAGGCATCGATGAAAAGCTGTTCAGCAAATATTGCCTACTGCCTATTTTTAAACGTAATAATTTACTTTACGT
TGCGACCAGTAACCCAACCAATATCGAAGCGATGGATGCGATTCGTTTCAACAGTAAAATGAATGTTGAAGCAATTGTGG
TTGAACATGACAAACTGGCCAAACTGATTGAGCAGAACTTCGCAGGTGAATCTGCTTTTGAGTTTGAAGATGATGACTTT
GAACTCAACTTTGACGAAGGCGACAACGAACAAGAACAAGCCACAGAAGATGAGCCACAGGGCGATGAATCCCCTATCGT
CAAATACATCAATAAATTACTGATTGATGCGATTCGTATGGGTGCCTCGGACTTACACTTTGAACCCTATGAAAAAACCT
ATCGCGTGCGTTACCGTGTCGATGGGGTCTTACGTCAAATTGCCAATCCACCATTACAGTTAGCCAATCGTATGGCATCA
CGCTTGAAAGTCATGTCGCAAATGGATATTTCTGAAAAACGTGTGCCGCAAGATGGTCGTATTAAACTTAAACTGTCTAA
GAATAAATCCATCGACTTCCGTGTTAACTCCTTGCCAACCTTGTTCGGTGAAAAAATTGTACTGCGTATTTTAGATCCAT
CTAGCGCCATGCTTGGGATTGATGCCTTGGGTTATGAGCCTGAACAAAAAGCTTTGTTTATGGAAGCTCTGGACAAACCG
CAAGGTATGTTACTGATCACAGGTCCAACAGGTTCAGGTAAAACGGTATCGCTCTATACAGGTTTAAACATTCTAAACCG
AGAAGACACCAACATTTCCACTGCCGAAGACCCTGTGGAAATTAACCTTGAAGGGATCAATCAGGTTAACGTCAACAATA
AAGTTGGTCTGACCTTTGCTGCAGCGCTGAAATCATTCTTACGTCAAGACCCTGACATTGTTATGGTGGGTGAGATTCGT
GACTTAGAAACAGCAGAAATTGCAATTAAAGCAGCACAAACGGGTCACATGGTGATGTCAACACTGCATACCAACAGTGC
ACCAGAAACTTTAACCCGTTTACGCAACATGGGAGTACCATCCTTTAACATTGCGACTTCAGTCAATATGGTGATTGCAC
AGCGTCTGGCTCGACGCCTATGCTCGTATTGCAAAACACCGTCAGATATTCCAAAACAAAGCTTATTGGAAATGGGTTTT
ACAGAAGCCGACTTGAACAATCCAGAGTTTCAGGTCTACCAACCAGTAGGCTGTGCTGAATGTCGTGAAGGCTACAAAGG
ACGTGTCGGTATTTATGAAGTGATGAAAGTCACACCAGAAATTTCCAAACTTATTATGGAGGATGGCAATGCCATCCAAA
TTGCTGAAGCATCGGCAAAATTGGGGTTTAACAATCTACGTCGCTCGGGGTTACTTAAGGTCATGCAGGGGGTGACTTCA
CTTCAAGAAGTCAATCGTGTGACCAGTGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.298

100

0.793
  pilB Acinetobacter baylyi ADP1

76.667

100

0.767
  pilB Legionella pneumophila strain ERS1305867

52.118

95.263

0.496
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio cholerae strain A1552

48.233

99.298

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

47.764

98.07

0.468
  pilB Vibrio campbellii strain DS40M4

50.385

91.228

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

35.981

100

0.389
  pilF Thermus thermophilus HB27

37.347

100

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.209

98.07

0.365

Multiple sequence alignment