Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACNS8M_RS01400 Genome accession   NZ_CP183239
Coordinates   308778..309638 (-) Length   286 a.a.
NCBI ID   WP_168541327.1    Uniprot ID   -
Organism   Acinetobacter sp. A2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 303778..314638
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNS8M_RS01375 (ACNS8M_01355) recN 303801..305462 (-) 1662 WP_168541323.1 DNA repair protein RecN -
  ACNS8M_RS01380 (ACNS8M_01360) - 305941..306264 (+) 324 WP_168417589.1 pyrimidine/purine nucleoside phosphorylase -
  ACNS8M_RS01385 (ACNS8M_01365) rlmB 306443..307192 (+) 750 WP_168541325.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACNS8M_RS01390 (ACNS8M_01370) - 307234..308163 (+) 930 WP_168417587.1 DMT family transporter -
  ACNS8M_RS01395 (ACNS8M_01375) coaE 308160..308768 (-) 609 WP_168417586.1 dephospho-CoA kinase -
  ACNS8M_RS01400 (ACNS8M_01380) pilD 308778..309638 (-) 861 WP_168541327.1 prepilin peptidase Machinery gene
  ACNS8M_RS01405 (ACNS8M_01385) pilC 309638..310864 (-) 1227 WP_168417584.1 type II secretion system F family protein Machinery gene
  ACNS8M_RS01410 (ACNS8M_01390) pilB 310895..312607 (-) 1713 WP_168417583.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACNS8M_RS01415 (ACNS8M_01395) tpiA 312885..313676 (+) 792 WP_168417582.1 triose-phosphate isomerase -
  ACNS8M_RS01420 (ACNS8M_01400) secG 313690..314019 (+) 330 WP_168417581.1 preprotein translocase subunit SecG -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32032.48 Da        Isoelectric Point: 8.0624

>NTDB_id=1104729 ACNS8M_RS01400 WP_168541327.1 308778..309638(-) (pilD) [Acinetobacter sp. A2]
MQELIAYFIQNPTALYIAVGLFSLCIGSFLNVVIYRTPKMMEQEWRTDCQMLLHPEQAIIDESKITLSKPASTCPKCHTP
IRWYQNIPVLSWLVLRGKCGHCQNPISMRYPLIEVLSAVCALIVVAVFGPTVQMLFGLILTYILIALTFIDFDTQLLPDR
YTLPLAALGLAINSYAIYTSPTSAIWGYVIGFLCLWVVYYLFKIITGKEGMGYGDFKLLAALGAWMGPMMLPLIVLLSSL
VGAIIGIILLKIRKENQPFAFGPYIAIAGWIAFLWGEQIMKVYLGQ

Nucleotide


Download         Length: 861 bp        

>NTDB_id=1104729 ACNS8M_RS01400 WP_168541327.1 308778..309638(-) (pilD) [Acinetobacter sp. A2]
ATGCAAGAATTAATCGCGTATTTTATTCAAAATCCAACTGCGTTGTACATCGCAGTTGGACTTTTTAGTTTATGTATTGG
CAGTTTTTTGAATGTGGTCATATACCGCACACCAAAAATGATGGAGCAGGAATGGCGTACAGACTGCCAAATGCTGTTAC
ATCCAGAACAAGCTATTATTGATGAAAGTAAAATCACACTGAGCAAACCCGCCTCGACTTGCCCTAAATGTCATACACCT
ATTCGTTGGTATCAGAATATTCCTGTACTGAGCTGGTTAGTTTTACGTGGCAAATGCGGGCACTGTCAAAACCCTATCAG
TATGCGCTATCCACTGATTGAAGTTTTAAGCGCAGTTTGTGCGCTGATTGTGGTTGCCGTATTTGGCCCAACTGTACAAA
TGCTGTTCGGTTTGATACTGACCTATATCTTAATTGCCCTCACCTTTATTGATTTCGACACTCAACTCTTGCCCGATCGC
TATACCCTACCTTTGGCTGCATTGGGCTTAGCAATCAACAGTTATGCCATCTACACCTCACCTACCTCTGCTATTTGGGG
TTATGTCATTGGCTTCTTATGCTTATGGGTAGTGTATTACCTGTTTAAAATTATTACAGGCAAAGAAGGCATGGGCTATG
GCGACTTTAAACTCCTTGCAGCCTTAGGCGCTTGGATGGGTCCAATGATGTTGCCGCTGATTGTGCTTTTATCTTCTTTG
GTTGGCGCTATTATTGGCATTATTCTGCTAAAAATCCGTAAAGAGAACCAACCTTTTGCATTTGGCCCTTATATTGCAAT
TGCAGGCTGGATTGCCTTTTTATGGGGCGAGCAAATCATGAAAGTTTATTTAGGACAATAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(138-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

82.105

99.65

0.818
  pilD Acinetobacter baumannii D1279779

81.754

99.65

0.815
  pilD Vibrio cholerae strain A1552

55.97

93.706

0.524
  pilD Vibrio campbellii strain DS40M4

53.16

94.056

0.5
  pilD Neisseria gonorrhoeae MS11

46.154

95.455

0.441

Multiple sequence alignment