Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACM0P2_RS15010 Genome accession   NZ_CP183209
Coordinates   3392720..3393832 (-) Length   370 a.a.
NCBI ID   WP_120961799.1    Uniprot ID   -
Organism   Alteromonas sp. OM2203     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3387720..3398832
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM0P2_RS14985 (ACM0P2_14985) - 3389295..3390023 (+) 729 WP_173359132.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACM0P2_RS14990 (ACM0P2_14990) gshB 3390139..3391089 (+) 951 WP_173359133.1 glutathione synthase -
  ACM0P2_RS14995 (ACM0P2_14995) - 3391202..3391768 (+) 567 WP_173359134.1 YqgE/AlgH family protein -
  ACM0P2_RS15000 (ACM0P2_15000) ruvX 3391761..3392192 (+) 432 WP_159117869.1 Holliday junction resolvase RuvX -
  ACM0P2_RS15005 (ACM0P2_15005) - 3392268..3392594 (-) 327 WP_159117868.1 DUF2007 domain-containing protein -
  ACM0P2_RS15010 (ACM0P2_15010) pilU 3392720..3393832 (-) 1113 WP_120961799.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACM0P2_RS15015 (ACM0P2_15015) pilT 3393841..3394884 (-) 1044 WP_159117866.1 type IV pilus twitching motility protein PilT Machinery gene
  ACM0P2_RS15020 (ACM0P2_15020) - 3394912..3395598 (+) 687 WP_159117865.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACM0P2_RS15025 (ACM0P2_15025) proC 3395649..3396470 (+) 822 WP_173359135.1 pyrroline-5-carboxylate reductase -
  ACM0P2_RS15030 (ACM0P2_15030) - 3396491..3397027 (+) 537 WP_159117863.1 YggT family protein -
  ACM0P2_RS15035 (ACM0P2_15035) - 3397117..3397590 (+) 474 WP_159117862.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40783.66 Da        Isoelectric Point: 6.0481

>NTDB_id=1104502 ACM0P2_RS15010 WP_120961799.1 3392720..3393832(-) (pilU) [Alteromonas sp. OM2203]
MLDSLLQKMVDSNASDLFITAGFPVSAKINGKLTPVTETATSEDASLALVHEAMTEKQQNEFHTSKECNFAIVREGIGRF
RCSAFWQRDQAGMVIRRIVTQIPKADDLGLPRVLKEIIMSKRGLVLFVGGTGTGKSTSLAALIGHRNENSHGHILTIEDP
IEFVHEHKNCVITQREVGIDTQSFDDALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERIM
HLAPKDQHDKLRFDLSLNIRAIVAQQLVPTPDGKGRVAAIEILLNSPLVRDLIQRNEIGALKEAMKKGKEQGMQSFDMAL
YDLYKAGKIDMDQALHHADSPNDLRLMIKLDTSDGSSLGTLSNVSIDMDD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=1104502 ACM0P2_RS15010 WP_120961799.1 3392720..3393832(-) (pilU) [Alteromonas sp. OM2203]
ATGTTAGATTCACTTTTACAAAAAATGGTCGACTCCAATGCGTCGGACTTGTTCATTACCGCGGGGTTTCCCGTTAGCGC
CAAGATAAACGGTAAGCTTACGCCGGTCACTGAAACGGCGACTTCTGAGGACGCCTCATTAGCGCTTGTTCATGAAGCCA
TGACCGAGAAGCAGCAAAATGAATTCCATACATCGAAGGAATGTAACTTTGCGATAGTGCGAGAAGGAATTGGCCGCTTT
CGTTGCTCTGCGTTTTGGCAGCGAGACCAAGCAGGCATGGTCATACGTCGAATTGTTACTCAGATACCAAAAGCAGATGA
TTTAGGTCTGCCGCGAGTATTGAAAGAAATCATTATGTCTAAGCGCGGTCTTGTTCTATTCGTGGGTGGAACCGGAACGG
GTAAATCTACTTCACTTGCTGCGCTTATTGGTCATAGAAATGAAAACTCTCATGGTCATATATTAACTATCGAAGACCCT
ATTGAATTTGTGCATGAGCACAAAAATTGCGTTATCACTCAGCGTGAAGTAGGTATCGATACGCAATCTTTTGACGATGC
GCTTAAAAGCTCGCTGCGTCAGGCGCCAGATGTCATTCTCATTGGTGAGATTCGTTCTATGGAAACCATGGAATATGCCA
TGTCGTTTGCCGATACGGGGCATTTGTGTGTGGCAACATTGCACGCTAACAATGCAAACCAAGCGATAGAGCGTATCATG
CATTTAGCGCCTAAAGATCAGCACGACAAGCTGCGCTTCGATTTAAGCCTAAATATTCGGGCTATTGTTGCCCAACAGCT
TGTTCCTACTCCGGATGGTAAAGGCCGAGTAGCTGCAATTGAAATACTGTTAAATTCACCATTAGTTCGCGATCTTATTC
AGCGAAATGAAATTGGCGCGCTAAAAGAGGCGATGAAGAAGGGCAAAGAGCAAGGTATGCAGAGCTTCGATATGGCCCTT
TATGATCTTTATAAAGCGGGCAAGATCGATATGGATCAAGCGCTGCATCATGCTGACTCGCCTAATGACTTACGCTTGAT
GATTAAACTTGATACTAGCGATGGATCTAGTTTGGGCACGCTATCTAATGTGTCGATTGATATGGATGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.647

100

0.608
  pilU Acinetobacter baylyi ADP1

60

94.595

0.568
  pilU Vibrio cholerae strain A1552

56.25

99.459

0.559
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.353

91.892

0.389
  pilT Acinetobacter nosocomialis M2

39.412

91.892

0.362
  pilT Acinetobacter baumannii D1279779

39.412

91.892

0.362
  pilT Acinetobacter baumannii strain A118

39.412

91.892

0.362

Multiple sequence alignment