Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   ACNH6H_RS09825 Genome accession   NZ_CP182947
Coordinates   2199862..2200344 (-) Length   160 a.a.
NCBI ID   WP_418317320.1    Uniprot ID   -
Organism   Piscinibacter sakaiensis strain 201-F6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2194862..2205344
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNH6H_RS09805 (ACNH6H_09805) - 2195281..2195637 (+) 357 WP_418317316.1 phage holin family protein -
  ACNH6H_RS09810 (ACNH6H_09810) - 2195650..2197260 (-) 1611 WP_418317317.1 M48 family metalloprotease -
  ACNH6H_RS09815 (ACNH6H_09815) moaC 2197407..2197934 (+) 528 WP_418317318.1 cyclic pyranopterin monophosphate synthase MoaC -
  ACNH6H_RS09820 (ACNH6H_09820) - 2197948..2199702 (-) 1755 WP_418317319.1 Wzy polymerase domain-containing protein -
  ACNH6H_RS09825 (ACNH6H_09825) pilE 2199862..2200344 (-) 483 WP_418317320.1 pilin Machinery gene
  ACNH6H_RS09830 (ACNH6H_09830) - 2200617..2201099 (-) 483 WP_418317321.1 pilin -
  ACNH6H_RS09835 (ACNH6H_09835) - 2201298..2202074 (-) 777 WP_418317322.1 MBL fold metallo-hydrolase -
  ACNH6H_RS09840 (ACNH6H_09840) - 2202350..2203003 (-) 654 WP_418317323.1 FHA domain-containing protein -
  ACNH6H_RS09845 (ACNH6H_09845) - 2203022..2203801 (-) 780 WP_418317324.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  ACNH6H_RS09850 (ACNH6H_09850) - 2203895..2205316 (-) 1422 WP_418317325.1 serine/threonine-protein kinase -

Sequence

Protein


Download         Length: 160 a.a.        Molecular weight: 16726.23 Da        Isoelectric Point: 8.0971

>NTDB_id=1103407 ACNH6H_RS09825 WP_418317320.1 2199862..2200344(-) (pilE) [Piscinibacter sakaiensis strain 201-F6]
MKRVQQGFTLIELMIVVAIIGILAAVALPAYQDYTVRAKVSEVVLAASSCRTTVTETVQSTTTTPMTATLFACNTDPATK
SSKYVSKVEVSNAGTITVTADGTDAGIKALGTNNALTLIPYKDESTPLTATDVGVNITKWVCGGTGTNIEKKYLPGSCRG

Nucleotide


Download         Length: 483 bp        

>NTDB_id=1103407 ACNH6H_RS09825 WP_418317320.1 2199862..2200344(-) (pilE) [Piscinibacter sakaiensis strain 201-F6]
ATGAAACGCGTTCAACAAGGTTTTACCCTGATCGAACTGATGATCGTTGTCGCGATCATCGGCATCCTCGCCGCGGTCGC
GCTGCCGGCTTACCAGGACTACACGGTCCGCGCCAAGGTGTCTGAAGTCGTGCTGGCCGCTTCGTCGTGCCGCACCACGG
TTACCGAGACCGTGCAGTCGACGACGACCACGCCGATGACGGCCACGCTGTTCGCCTGCAATACCGATCCGGCGACGAAG
AGCTCCAAATATGTCAGCAAGGTCGAAGTTTCCAACGCCGGTACGATCACGGTGACCGCCGATGGCACGGACGCCGGCAT
CAAGGCGCTGGGCACCAACAACGCGCTGACGCTGATCCCGTACAAGGACGAGTCGACGCCGCTGACCGCGACCGATGTTG
GTGTCAACATCACGAAGTGGGTCTGCGGTGGCACCGGCACCAACATCGAGAAGAAGTATCTGCCGGGTTCGTGCCGCGGC
TGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-159)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

41.361

100

0.494
  comP Acinetobacter baylyi ADP1

41.86

100

0.45
  pilE Neisseria gonorrhoeae strain FA1090

39.884

100

0.431
  pilA/pilA1 Eikenella corrodens VA1

39.053

100

0.412
  pilE Neisseria gonorrhoeae MS11

38.15

100

0.412
  pilA Ralstonia pseudosolanacearum GMI1000

37.356

100

0.406
  pilA2 Legionella pneumophila str. Paris

38.854

98.125

0.381
  pilA2 Legionella pneumophila strain ERS1305867

38.854

98.125

0.381

Multiple sequence alignment