Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACNH6B_RS01045 Genome accession   NZ_CP182944
Coordinates   223220..224926 (-) Length   568 a.a.
NCBI ID   WP_418357949.1    Uniprot ID   -
Organism   Shewanella basaltis strain KCTC 22121     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 218220..229926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNH6B_RS01015 (ACNH6B_01015) mutT 218791..219189 (-) 399 WP_418357944.1 8-oxo-dGTP diphosphatase MutT -
  ACNH6B_RS01020 (ACNH6B_01020) yacG 219212..219421 (-) 210 WP_248970754.1 DNA gyrase inhibitor YacG -
  ACNH6B_RS01025 (ACNH6B_01025) zapD 219500..220234 (-) 735 WP_418357945.1 cell division protein ZapD -
  ACNH6B_RS01030 (ACNH6B_01030) coaE 220227..220856 (-) 630 WP_418357946.1 dephospho-CoA kinase -
  ACNH6B_RS01035 (ACNH6B_01035) pilD 220853..221779 (-) 927 WP_418357947.1 prepilin peptidase Machinery gene
  ACNH6B_RS01040 (ACNH6B_01040) pilC 221800..223065 (-) 1266 WP_418357948.1 type II secretion system F family protein Machinery gene
  ACNH6B_RS01045 (ACNH6B_01045) pilB 223220..224926 (-) 1707 WP_418357949.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACNH6B_RS01050 (ACNH6B_01050) pilE 225018..225461 (-) 444 WP_418357950.1 pilin Machinery gene
  ACNH6B_RS01055 (ACNH6B_01055) nadC 226295..227173 (-) 879 WP_248970760.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACNH6B_RS01060 (ACNH6B_01060) ampD 227370..227942 (+) 573 WP_418357951.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACNH6B_RS01065 (ACNH6B_01065) ampE 228241..229092 (+) 852 WP_418357952.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62908.16 Da        Isoelectric Point: 5.2658

>NTDB_id=1103382 ACNH6B_RS01045 WP_418357949.1 223220..224926(-) (pilB) [Shewanella basaltis strain KCTC 22121]
MSSAAVHLGLSTLFIRKGLLNEDQISTAISESRKNKQSLVTTLINTKQLSAREIAELCYEEYGTPLLDLNEFDILGISEE
LLNKKLIEKHKCIPLFKRGNRLYIATSDPTNIAALEDFQFSLGLHAEAILVEDDKLLKALEKIIEDDISGLDLAGADEEA
LANMEVTDTERRDEQNSDSSDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLSGRISAR
LKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEDDQRQLYEEMLAKPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPACKQPEDIPAAELQRLGFQ
EEDISKGISVFKPVGCELCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIAKQAKQQGMRDLRQSGLLKVIQGVTSIA
EINRVTSF

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=1103382 ACNH6B_RS01045 WP_418357949.1 223220..224926(-) (pilB) [Shewanella basaltis strain KCTC 22121]
ATGTCATCAGCTGCAGTTCATTTAGGATTATCAACTTTATTTATCCGTAAAGGCCTTTTAAATGAAGATCAAATATCAAC
TGCAATTTCCGAATCCCGAAAAAATAAACAATCCTTAGTCACCACATTGATTAATACTAAACAGCTCTCTGCTCGTGAAA
TTGCAGAACTCTGTTATGAAGAATACGGCACGCCATTACTTGATTTAAATGAATTTGATATCTTAGGTATCTCTGAAGAG
TTACTTAACAAAAAGCTCATAGAAAAACACAAATGTATCCCCTTGTTCAAACGAGGTAACCGCCTTTATATTGCCACTTC
AGATCCTACAAACATTGCTGCATTAGAAGATTTCCAATTTAGTTTAGGCTTACATGCTGAAGCGATTCTCGTTGAAGACG
ATAAACTGCTTAAAGCGCTAGAAAAAATTATTGAAGATGACATTTCAGGCCTAGATTTAGCAGGTGCAGATGAAGAAGCA
CTTGCCAACATGGAAGTCACTGACACTGAAAGACGCGACGAGCAAAATAGTGACAGCAGCGACGATGCACCGATTGTTAT
CTACATCAATAAAATTCTTACCGATGCCATCCGTAAAGGCGCCTCAGATTTACATTTTGAGCCGTATGAAAAGCGGTACC
GCATTCGTTTTCGTATTGACGGCATTTTACATGAAGTGTCTGAGCCACCTGTTAATCTATCTGGGCGTATTTCTGCTCGC
TTAAAGGTAATGTCTAAACTAGATATTGCAGAGCGCCGCGTACCGCAAGATGGCCGTATTAAAATGAAGCTATCGCGCAC
CAAGTCCATCGATTTTCGCGTGAGTACCCTGCCCACGATTTGGGGCGAGAAAATTGTAATGCGTATTCTGGACTCATCTT
CTGCGCAATTAGGTATTGAAAAGCTGGGTTACGAAGACGATCAACGCCAGCTATACGAAGAAATGCTTGCCAAACCTCAG
GGGATGATTTTAGTCACTGGCCCAACGGGGTCAGGTAAAACCGTGTCCCTGTATACTGGGCTTAATATCCTTAACACCGA
AGAGCGAAATATCTCAACCGCCGAAGATCCGGTAGAGATTAACCTAGAAGGGGTTAACCAGGTTCACATTAATTTAAAAG
CCGGCTTAACCTTCGCCTCGGCCTTACGCTCATTTTTACGGCAAGACCCAGATGTGGTCATGGTAGGTGAAATCCGCGAC
CTAGAAACCGCCGAAATTGCCATTAAAGCGGCGCAAACCGGTCACTTAGTGTTATCGACACTGCACACTAACTCAGCAGC
CGAAACCCTAACCCGGTTAATTAACATGGGTGTGCCCGGTTATAACATTGCCAGTTCTGTCAATTTAATTATTGCCCAGC
GCCTTGCAAGGCGTTTATGCCCCGCATGCAAACAGCCAGAAGACATCCCCGCGGCAGAGCTACAACGCCTGGGTTTTCAA
GAGGAAGACATTAGTAAAGGGATCAGCGTATTTAAACCAGTAGGCTGTGAGCTATGTTCGGGTGGTTATAAAGGCCGTGT
CGGTATTTACGAAGTGATGAAAATGAGTGATGAAATCGCCCGCACTATTATGGAAGGCGGCAACTCACTGCAAATTGCCA
AGCAGGCTAAACAGCAAGGTATGCGCGATTTACGCCAATCTGGGTTACTCAAAGTAATACAAGGCGTAACCAGTATTGCA
GAAATAAACCGTGTCACCAGCTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-143)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.18

98.768

0.585
  pilB Acinetobacter baumannii D1279779

58.541

98.944

0.579
  pilB Legionella pneumophila strain ERS1305867

56.94

98.944

0.563
  pilB Vibrio parahaemolyticus RIMD 2210633

52.197

100

0.523
  pilB Vibrio cholerae strain A1552

53.61

97.535

0.523
  pilB Vibrio campbellii strain DS40M4

50.694

100

0.514
  pilF Neisseria gonorrhoeae MS11

47.795

99.824

0.477
  pilF Thermus thermophilus HB27

38.011

99.12

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.043

97.183

0.37

Multiple sequence alignment