Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACM613_RS05775 Genome accession   NZ_CP182485
Coordinates   1232489..1233700 (-) Length   403 a.a.
NCBI ID   WP_254856747.1    Uniprot ID   -
Organism   Opacimonas sp. LMIT016     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1227489..1238700
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM613_RS05745 (ACM613_05745) mutT 1228618..1229022 (+) 405 WP_254856739.1 8-oxo-dGTP diphosphatase MutT -
  ACM613_RS05750 (ACM613_05750) - 1229012..1229884 (-) 873 WP_254856741.1 aspartoacylase -
  ACM613_RS05755 (ACM613_05755) yacG 1229890..1230120 (-) 231 WP_254856743.1 DNA gyrase inhibitor YacG -
  ACM613_RS05760 (ACM613_05760) zapD 1230120..1230872 (-) 753 WP_254856744.1 cell division protein ZapD -
  ACM613_RS05765 (ACM613_05765) coaE 1230912..1231526 (-) 615 WP_254856745.1 dephospho-CoA kinase -
  ACM613_RS05770 (ACM613_05770) pilD 1231526..1232449 (-) 924 WP_254856746.1 prepilin peptidase Machinery gene
  ACM613_RS05775 (ACM613_05775) pilC 1232489..1233700 (-) 1212 WP_254856747.1 type II secretion system F family protein Machinery gene
  ACM613_RS05780 (ACM613_05780) - 1233789..1234187 (-) 399 WP_418026110.1 pilin -
  ACM613_RS05785 (ACM613_05785) nadC 1234356..1235273 (-) 918 WP_254856749.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACM613_RS05790 (ACM613_05790) - 1235270..1235848 (-) 579 WP_254856750.1 retropepsin-like aspartic protease family protein -
  ACM613_RS05795 (ACM613_05795) ampD 1235876..1236448 (+) 573 WP_418026122.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACM613_RS05800 (ACM613_05800) ampE 1236445..1237269 (+) 825 WP_254856752.1 regulatory signaling modulator protein AmpE -
  ACM613_RS05805 (ACM613_05805) - 1237478..1238233 (+) 756 WP_254856753.1 GntR family transcriptional regulator -

Sequence

Protein


Download         Length: 403 a.a.        Molecular weight: 43887.68 Da        Isoelectric Point: 8.8742

>NTDB_id=1101631 ACM613_RS05775 WP_254856747.1 1232489..1233700(-) (pilC) [Opacimonas sp. LMIT016]
MAQSSFMYYWQGADRRGNKVKGKLTAASPKDVKLILRKQGISAQHIKLVKKSWLSVAKDKLNSEDICVLTRQIATMLNAG
ISIMQTLDMLAKGHAKAEARDVLRSIASEVKAGNPLSVALRKYPKHFGDLYCDLVASGEQSGALEQIFERIALYQEKAEA
LKSKIIKAMFYPIAVVVVALIVTTILLIYVVPQFQDIFASFGAELPVFTQLVLNISQFLQDYGFAILISFIACFWGSKKS
IERSPAVADKVDAQILKLPIVGEILEKAAIARFARTLATTFAAGVPFIQALDSAAGAAGNAIYRDAIQFVKKEVSGGSQM
HIAMRATTLFPDMVVQMIAIGEESGSVDDMLGKISDIYERQVEDMVDGLTSLLEPLIMAVLGVVIGGLIVAMYLPIFQMG
NVV

Nucleotide


Download         Length: 1212 bp        

>NTDB_id=1101631 ACM613_RS05775 WP_254856747.1 1232489..1233700(-) (pilC) [Opacimonas sp. LMIT016]
ATGGCCCAGTCTTCATTTATGTATTATTGGCAAGGCGCTGACCGTCGCGGTAATAAAGTAAAAGGAAAATTGACCGCCGC
CTCGCCCAAAGACGTCAAACTCATATTACGCAAGCAAGGGATCAGTGCGCAACACATTAAATTAGTCAAAAAATCTTGGC
TCTCAGTTGCTAAAGATAAACTCAATTCAGAAGATATCTGTGTCCTCACTCGACAAATAGCCACAATGCTCAATGCTGGT
ATAAGCATTATGCAGACACTCGATATGTTAGCTAAAGGCCATGCAAAAGCAGAAGCCCGAGACGTATTACGGAGCATCGC
TAGTGAGGTCAAAGCGGGGAATCCGCTTAGTGTCGCTTTGCGCAAATACCCCAAACACTTTGGTGACCTATATTGTGATT
TGGTAGCAAGTGGTGAGCAATCCGGTGCACTGGAGCAGATTTTTGAACGCATCGCACTTTATCAAGAAAAAGCAGAAGCC
TTAAAATCTAAAATAATAAAAGCCATGTTCTACCCTATTGCGGTTGTGGTCGTCGCATTGATTGTCACAACGATTTTATT
GATTTACGTGGTGCCGCAATTCCAGGATATTTTTGCTAGCTTTGGCGCAGAACTTCCTGTTTTTACACAATTGGTATTAA
ATATATCCCAATTTTTGCAAGATTACGGCTTTGCCATTTTAATCAGTTTTATTGCTTGTTTTTGGGGGAGCAAAAAAAGT
ATAGAGCGTTCACCTGCTGTGGCAGATAAAGTCGATGCTCAAATATTAAAACTCCCTATTGTCGGCGAAATCCTAGAAAA
AGCCGCCATTGCTCGCTTTGCCAGAACCTTAGCCACCACTTTTGCCGCGGGTGTGCCTTTTATTCAAGCTTTAGATTCAG
CGGCTGGGGCAGCTGGCAATGCCATCTACCGCGATGCTATTCAATTTGTTAAAAAAGAGGTATCAGGTGGTTCGCAAATG
CACATTGCAATGCGAGCGACGACCTTGTTTCCTGATATGGTCGTGCAGATGATTGCTATCGGTGAAGAATCAGGATCTGT
CGATGACATGCTAGGAAAAATATCAGATATTTACGAGCGCCAAGTGGAGGATATGGTAGATGGTTTAACCAGTTTATTAG
AGCCCCTAATCATGGCTGTGTTGGGCGTCGTTATCGGTGGCTTAATCGTTGCGATGTATTTGCCTATCTTCCAAATGGGC
AATGTGGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-395)

Type II secretion system (T2SS), protein F (T2SSF)

(70-192)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

52.956

100

0.534
  pilC Acinetobacter baumannii D1279779

50.372

100

0.504
  pilC Acinetobacter baylyi ADP1

49.749

98.759

0.491
  pilC Legionella pneumophila strain ERS1305867

46.75

99.256

0.464
  pilC Vibrio cholerae strain A1552

45.96

98.263

0.452
  pilC Vibrio campbellii strain DS40M4

45.137

99.504

0.449
  pilG Neisseria meningitidis 44/76-A

42.569

98.511

0.419
  pilG Neisseria gonorrhoeae MS11

42.569

98.511

0.419

Multiple sequence alignment