Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   H9T68_RS23665 Genome accession   NZ_CP181346
Coordinates   5215705..5216196 (+) Length   163 a.a.
NCBI ID   WP_280190036.1    Uniprot ID   -
Organism   Delftia sp. PS-11     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5210705..5221196
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9T68_RS23645 (H9T68_023640) sucD 5211763..5212656 (+) 894 WP_280190033.1 succinate--CoA ligase subunit alpha -
  H9T68_RS23650 (H9T68_023645) - 5212821..5213513 (+) 693 WP_280190034.1 TerC family protein -
  H9T68_RS23655 (H9T68_023650) - 5213775..5214533 (+) 759 WP_280190108.1 PP2C family protein-serine/threonine phosphatase -
  H9T68_RS23660 (H9T68_023655) - 5214773..5215501 (+) 729 WP_280190035.1 FHA domain-containing protein -
  H9T68_RS23665 (H9T68_023660) pilA 5215705..5216196 (+) 492 WP_280190036.1 pilin Machinery gene
  H9T68_RS23670 (H9T68_023665) - 5216292..5218235 (-) 1944 WP_280190037.1 tetratricopeptide repeat protein -
  H9T68_RS23675 (H9T68_023670) - 5218252..5218548 (-) 297 WP_280190038.1 hypothetical protein -
  H9T68_RS23680 (H9T68_023675) moaC 5218586..5219059 (-) 474 WP_280190039.1 cyclic pyranopterin monophosphate synthase MoaC -
  H9T68_RS23685 (H9T68_023680) - 5219194..5220834 (+) 1641 WP_280190109.1 M48 family metalloprotease -
  H9T68_RS23690 (H9T68_023685) - 5220808..5221155 (-) 348 WP_280190041.1 phage holin family protein -

Sequence

Protein


Download         Length: 163 a.a.        Molecular weight: 16189.49 Da        Isoelectric Point: 9.0251

>NTDB_id=1099820 H9T68_RS23665 WP_280190036.1 5215705..5216196(+) (pilA) [Delftia sp. PS-11]
MKRSIQQGFTLIELMIVVAIIGILAAVALPAYQDYTVRAKVTEGLGLAASAKVAVAENAANGKPYAAGWVAPNATNNVAS
VAIDQAAGFITITYASGVAGGGTLILNPIDGPKATGTAFSGGTASASTIPTSGSISWNCRAASSSGQGTAGTILAKYVPA
ECR

Nucleotide


Download         Length: 492 bp        

>NTDB_id=1099820 H9T68_RS23665 WP_280190036.1 5215705..5216196(+) (pilA) [Delftia sp. PS-11]
ATGAAGCGTTCCATCCAGCAAGGTTTCACCTTGATCGAACTGATGATCGTCGTGGCGATCATCGGTATTTTGGCCGCCGT
GGCGCTGCCGGCTTACCAGGATTACACGGTGCGTGCAAAGGTCACCGAAGGTCTGGGCCTGGCCGCATCGGCCAAGGTGG
CTGTGGCTGAAAACGCAGCCAACGGCAAGCCTTACGCAGCCGGCTGGGTCGCGCCCAACGCGACCAACAACGTGGCCAGC
GTGGCGATTGACCAAGCTGCAGGTTTCATCACCATCACCTACGCCTCCGGCGTGGCTGGTGGCGGCACCCTGATTCTGAA
CCCGATCGATGGCCCCAAGGCGACCGGCACTGCCTTCTCCGGCGGCACGGCATCGGCATCGACCATCCCCACCTCCGGCT
CTATCAGCTGGAACTGCCGTGCTGCCAGCTCTTCTGGCCAAGGTACTGCAGGCACCATCCTGGCCAAGTACGTTCCGGCA
GAATGCCGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-29)

Pilin (bacterial filament) (Pilin)

(37-163)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

50.289

100

0.534
  pilA2 Legionella pneumophila str. Paris

52.147

100

0.521
  pilA2 Legionella pneumophila strain ERS1305867

52.147

100

0.521
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.129

100

0.46
  comP Acinetobacter baylyi ADP1

41.04

100

0.436
  pilE Neisseria gonorrhoeae MS11

37.427

100

0.393
  pilA/pilA1 Eikenella corrodens VA1

36.158

100

0.393
  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.31

100

0.374

Multiple sequence alignment