Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACM660_RS01980 Genome accession   NZ_CP181170
Coordinates   414311..416023 (-) Length   570 a.a.
NCBI ID   WP_005200490.1    Uniprot ID   -
Organism   Acinetobacter sp. SEK541     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 409311..421023
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM660_RS01950 (ACM660_01950) - 409432..409758 (+) 327 WP_004802302.1 pyrimidine/purine nucleoside phosphorylase -
  ACM660_RS01955 (ACM660_01955) rlmB 409889..410638 (+) 750 WP_004802304.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACM660_RS01960 (ACM660_01960) - 410669..411580 (+) 912 WP_412781686.1 DMT family transporter -
  ACM660_RS01965 (ACM660_01965) coaE 411577..412182 (-) 606 WP_005200493.1 dephospho-CoA kinase -
  ACM660_RS01970 (ACM660_01970) pilD 412184..413044 (-) 861 WP_005200492.1 prepilin peptidase Machinery gene
  ACM660_RS01975 (ACM660_01975) pilC 413057..414286 (-) 1230 WP_005200491.1 type II secretion system F family protein Machinery gene
  ACM660_RS01980 (ACM660_01980) pilB 414311..416023 (-) 1713 WP_005200490.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACM660_RS01985 (ACM660_01985) tpiA 416395..417186 (+) 792 WP_005200489.1 triose-phosphate isomerase -
  ACM660_RS01990 (ACM660_01990) secG 417199..417528 (+) 330 WP_004802318.1 preprotein translocase subunit SecG -
  ACM660_RS02010 (ACM660_02010) rimP 418157..418681 (+) 525 WP_004802320.1 ribosome maturation factor RimP -
  ACM660_RS02015 (ACM660_02015) nusA 418719..420203 (+) 1485 WP_005200488.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63404.66 Da        Isoelectric Point: 5.0756

>NTDB_id=1099376 ACM660_RS01980 WP_005200490.1 414311..416023(-) (pilB) [Acinetobacter sp. SEK541]
MSGLHTSPKFSGFIRRLVEDGHMSTTSMQIAIDSAKKAQQDIVAHLIEQHRLSPLTIAETIAAEFGEPLFDLSVYDTALL
PRDLADNKLIQKHKVAPLLQRGQILYVATSNPTNIEALDAIRFNTKLNIEPIIVEHTKLEKLIEQQFGDSGSFDFGESED
FDLDIDVNQDPSREEDEAPQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQITNPPLQMATRLAS
RLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKALFMDALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDVIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCNQCKEAIEVPKQSLLELGF
TEQDLNHPEFQIFQPIGCAECREGYKGRVGIYEVMKVTPEISRLIMEDGNALQIAAASEQAGFNNLRRSGLKKVMQGITS
LQEINRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1099376 ACM660_RS01980 WP_005200490.1 414311..416023(-) (pilB) [Acinetobacter sp. SEK541]
ATGTCAGGATTACATACGTCTCCAAAGTTTTCGGGGTTTATTCGACGGTTGGTTGAAGATGGACACATGTCCACCACAAG
CATGCAAATCGCAATTGATTCGGCTAAAAAAGCACAACAAGACATCGTTGCACATCTGATTGAACAGCATCGTTTATCTC
CCCTAACCATTGCTGAAACGATTGCAGCCGAATTCGGTGAACCACTTTTCGATCTGTCTGTATACGATACGGCTTTGCTA
CCTCGCGATCTGGCAGATAACAAACTCATTCAGAAACATAAAGTTGCACCGTTACTACAACGTGGGCAAATTTTATATGT
CGCGACCAGCAATCCGACCAATATTGAAGCATTAGATGCCATTCGCTTTAATACCAAACTCAATATTGAGCCCATCATTG
TTGAGCACACAAAACTTGAAAAACTGATTGAACAACAGTTTGGCGATTCTGGCAGTTTCGATTTTGGTGAAAGTGAAGAC
TTTGATCTCGATATTGATGTTAACCAAGACCCCTCTAGAGAAGAGGATGAGGCCCCACAAGGCGATGAGTCTCCAATTGT
CAAATACATCAATAAACTGTTGATTGATGCGATTCGTATGGGGGCATCCGATTTACATTTCGAACCTTATGAAAAGATGT
ACCGTGTTCGCTATCGTGTCGATGGCGTACTCCGTCAAATTACCAATCCGCCTTTACAAATGGCCACTCGTCTCGCCTCT
CGTTTAAAAGTCATGTCACAAATGGACATTTCTGAAAAACGAATGCCTCAAGATGGACGTATTAAACTTAAACTGTCCAA
GACCAAAGCCATCGATTTCCGTGTCAACTCACTACCAACACTGTTTGGCGAAAAGTTGGTACTGCGTATTCTGGACCCTT
CCAGTGCCATGTTGGGGATTGAAGCACTCGGTTATGAGGATGAGCAAAAAGCCTTATTTATGGATGCACTGGATAAGCCC
CAAGGCATGTTACTGATTACAGGCCCAACAGGTTCAGGTAAAACCGTGTCTTTGTATACCGGTTTAAACATCCTCAATAC
CGAAGACACCAATATTTCGACCGCCGAAGATCCAGTTGAAATTAACTTGGAAGGAATTAATCAGGTTAACGTCAATCCGA
AAACAGGACTCACCTTCTCTGCTGCGCTGAAAGCCTTTTTACGTCAAGATCCAGACGTGATCATGGTCGGGGAGATCCGT
GATCTGGAAACTGCCGAAATTGCCATTAAAGCCGCACAAACAGGTCATATGGTGATGTCAACCCTACATACCAACAGTGC
ACCAGAAACCCTAACCCGTTTACGCAATATGGGGGTCCCTTCTTTCAACATTGCAACTTCTGTCAATCTGGTGATTGCGC
AACGCTTAGCACGACGTTTATGTAATCAATGTAAAGAAGCCATCGAGGTCCCCAAACAAAGCCTATTAGAACTTGGTTTT
ACCGAACAGGATTTAAATCATCCAGAATTCCAAATTTTTCAACCCATCGGTTGTGCTGAGTGCCGTGAAGGCTATAAAGG
TCGTGTGGGGATTTACGAAGTCATGAAGGTCACGCCAGAAATTTCTAGACTGATTATGGAAGATGGCAATGCCTTACAAA
TTGCGGCTGCATCAGAGCAAGCAGGGTTTAATAATCTCCGCCGTTCAGGTTTGAAAAAAGTCATGCAGGGAATTACCTCT
TTGCAAGAAATCAATCGAGTAACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.786

100

0.819
  pilB Acinetobacter baylyi ADP1

76.182

100

0.763
  pilB Legionella pneumophila strain ERS1305867

52.768

95.088

0.502
  pilF Neisseria gonorrhoeae MS11

48.668

98.772

0.481
  pilB Vibrio cholerae strain A1552

49.365

96.667

0.477
  pilB Vibrio parahaemolyticus RIMD 2210633

51.931

90.877

0.472
  pilB Vibrio campbellii strain DS40M4

51.445

91.053

0.468
  pilF Thermus thermophilus HB27

37.5

98.246

0.368
  pilB/pilB1 Synechocystis sp. PCC 6803

33.713

100

0.363

Multiple sequence alignment