Detailed information NTG1099374

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACM660_RS01970
• Genome accession: NZ_CP181170
• Coordinates: 412184..413044 (-)
• Length: 286 a.a.
• NCBI ID: WP_005200492.1
• Uniprot ID: N9TF05
• Organism: Acinetobacter sp. SEK541
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 407184..418044

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACM660_RS01945 (ACM660_01945)	recN	407199..408857 (-)	1659	WP_005200507.1	DNA repair protein RecN	-
ACM660_RS01950 (ACM660_01950)	-	409432..409758 (+)	327	WP_004802302.1	pyrimidine/purine nucleoside phosphorylase	-
ACM660_RS01955 (ACM660_01955)	rlmB	409889..410638 (+)	750	WP_004802304.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
ACM660_RS01960 (ACM660_01960)	-	410669..411580 (+)	912	WP_412781686.1	DMT family transporter	-
ACM660_RS01965 (ACM660_01965)	coaE	411577..412182 (-)	606	WP_005200493.1	dephospho-CoA kinase	-
ACM660_RS01970 (ACM660_01970)	pilD	412184..413044 (-)	861	WP_005200492.1	prepilin peptidase	Machinery gene
ACM660_RS01975 (ACM660_01975)	pilC	413057..414286 (-)	1230	WP_005200491.1	type II secretion system F family protein	Machinery gene
ACM660_RS01980 (ACM660_01980)	pilB	414311..416023 (-)	1713	WP_005200490.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ACM660_RS01985 (ACM660_01985)	tpiA	416395..417186 (+)	792	WP_005200489.1	triose-phosphate isomerase	-
ACM660_RS01990 (ACM660_01990)	secG	417199..417528 (+)	330	WP_004802318.1	preprotein translocase subunit SecG	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 31892.21 Da
• Isoelectric Point: 6.8233

>NTDB_id=1099374 ACM660_RS01970 WP_005200492.1 412184..413044(-) (pilD) [Acinetobacter sp. SEK541]
MQSLFSYFLENLTALYIAVGIFSLCIGSFLNVVIFRTPRMMEQEWQQECQMLLHPEQPIIDQSKLTLSQPASTCPKCDSP
IRWYQNIPVISWLALRGKCGTCQNPISIRYPLIELLTMICSLVVVAVFGATIQMLFGLILTWVLIALTFIDFDTQLLPDR
FTLPLAALGLGTNSYAIYTTATAAIWGYLIGFLCLWIVYYIFKLITGKEGMGYGDFKLLAALGAWMGPMLLPLIVLLSSM
LGAIIGIILIKVRGENQPFAFGPYIAIAGWIAFLWGDQIMKVYLGG

Nucleotide

• Length: 861 bp

>NTDB_id=1099374 ACM660_RS01970 WP_005200492.1 412184..413044(-) (pilD) [Acinetobacter sp. SEK541]
ATGCAATCTTTATTTTCTTATTTTCTCGAGAACCTAACTGCACTTTATATTGCAGTTGGGATTTTCAGCTTATGTATTGG
CAGTTTTTTAAATGTTGTCATTTTTCGTACTCCCCGCATGATGGAGCAGGAGTGGCAGCAAGAATGTCAAATGCTGTTAC
ACCCAGAACAACCCATCATTGATCAAAGTAAACTGACTTTAAGCCAACCAGCCTCAACCTGTCCGAAATGTGACTCGCCT
ATTCGTTGGTATCAAAATATCCCAGTGATTAGTTGGTTAGCTCTTCGTGGTAAATGTGGGACGTGCCAGAATCCGATCAG
TATCCGTTATCCACTGATTGAATTACTCACGATGATCTGTTCTTTGGTTGTAGTTGCAGTCTTCGGTGCAACCATCCAAA
TGCTGTTTGGTCTAATCTTAACTTGGGTACTGATTGCCTTAACGTTTATTGATTTTGACACCCAACTGCTGCCTGATCGT
TTTACCCTACCCTTAGCTGCTTTAGGTTTGGGAACAAATAGCTATGCGATTTACACGACAGCGACCGCCGCAATTTGGGG
TTATTTAATTGGTTTCCTGTGCCTTTGGATTGTTTATTATATTTTTAAACTGATTACAGGCAAGGAAGGTATGGGCTACG
GTGACTTCAAACTCCTTGCAGCCTTAGGCGCTTGGATGGGGCCAATGCTGCTGCCTTTAATTGTGTTACTCTCTTCAATG
CTTGGCGCAATTATTGGTATTATCTTGATAAAAGTGCGCGGTGAAAATCAACCTTTTGCATTTGGTCCTTATATTGCGAT
TGCAGGTTGGATTGCATTTTTATGGGGTGATCAGATTATGAAAGTTTATCTAGGTGGTTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (138-247)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-127)

3D structure

• AlphaFold DB: N9TF05

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter nosocomialis M2	83.566	100	0.836
pilD	Acinetobacter baumannii D1279779	82.867	100	0.829
pilD	Vibrio cholerae strain A1552	54.104	93.706	0.507
pilD	Vibrio campbellii strain DS40M4	52.434	93.357	0.49
pilD	Neisseria gonorrhoeae MS11	47.348	92.308	0.437