Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACM0O0_RS17155 Genome accession   NZ_CP180689
Coordinates   3666651..3668363 (+) Length   570 a.a.
NCBI ID   WP_416040747.1    Uniprot ID   -
Organism   Acinetobacter lactucae isolate nanjing     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3661651..3673363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM0O0_RS17120 nusA 3662539..3664023 (-) 1485 WP_002115134.1 transcription termination factor NusA -
  ACM0O0_RS17125 rimP 3664061..3664585 (-) 525 WP_016144169.1 ribosome maturation factor RimP -
  ACM0O0_RS17145 secG 3665223..3665552 (-) 330 WP_002115012.1 preprotein translocase subunit SecG -
  ACM0O0_RS17150 tpiA 3665565..3666359 (-) 795 WP_057104882.1 triose-phosphate isomerase -
  ACM0O0_RS17155 pilB 3666651..3668363 (+) 1713 WP_416040747.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACM0O0_RS17160 pilC 3668391..3669617 (+) 1227 WP_416040748.1 type II secretion system F family protein Machinery gene
  ACM0O0_RS17165 pilD 3669617..3670477 (+) 861 WP_416040749.1 prepilin peptidase Machinery gene
  ACM0O0_RS17170 coaE 3670479..3671075 (+) 597 WP_216946733.1 dephospho-CoA kinase -
  ACM0O0_RS17175 - 3671072..3671986 (-) 915 WP_062849622.1 DMT family transporter -
  ACM0O0_RS17180 rlmB 3672022..3672771 (-) 750 WP_416040750.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACM0O0_RS17185 - 3672874..3673200 (-) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63737.26 Da        Isoelectric Point: 5.0294

>NTDB_id=1097477 ACM0O0_RS17155 WP_416040747.1 3666651..3668363(+) (pilB) [Acinetobacter lactucae isolate nanjing]
MSVIHTSPKFSGFFRRLVEEKHVSATTMQTALDAAKRAKRDTVAYLIEEIHLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVELVIVEHYKLEKILTQQFTEESSFEFSDEEF
DLDVDLEAPPSQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDVLGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKIPADIPKQSLLEMGF
TEQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1097477 ACM0O0_RS17155 WP_416040747.1 3666651..3668363(+) (pilB) [Acinetobacter lactucae isolate nanjing]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAACGAC
CATGCAAACAGCATTAGATGCAGCAAAAAGAGCTAAACGGGATACGGTTGCATACCTGATTGAAGAGATTCATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGACGTTTACGATACCAGCCAAATT
CCTAAAGATTTGGTTGATCAAAAACTGATTTTAAAACATCGGATCTTACCTCTTATACAAAGAGGACAGATTTTATATGT
CGCGACCAGCAATCCAAGTAATATTGAAGCGATTGATGCCATTCGCTTCAATAGTAAGCTTATGGTTGAACTAGTTATTG
TCGAGCACTATAAGCTTGAGAAAATTCTGACGCAACAATTTACCGAAGAAAGCAGTTTCGAGTTTAGTGATGAAGAGTTT
GATCTTGATGTAGACCTTGAAGCACCACCATCACAAGAGGATGAGGAAGAAACACCCCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCGATTCGTATGGGGGCTTCAGACTTACATTTTGAACCCTATGAAAAATCCT
ATCGGGTCCGTTACCGAGTCGACGGCGTGTTGCGTCAGATTGCAAATCCGCCTTTGCAATTGGCTAATCGTTTAGCGTCC
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAAAAACGGGTGCCTCAAGATGGTCGTATCAAACTTAAGCTCTCAAA
AACTAAAGCAATCGATTTTCGTGTGAACTCACTTCCAACCTTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CTAGCGCGATGCTCGGAATTGATGTTTTAGGATATGAAGAAGATCAGAAAGCCCTTTTTTTAGAAGCACTCGATAAACCA
CAAGGCATGCTTTTAATTACAGGGCCTACTGGCTCTGGTAAAACGGTATCTTTATATACAGGCTTAAATATTTTAAATAC
TGAAAGCTCTAATATTTCCACTGCCGAAGATCCAGTCGAAATCAACCTTGAAGGTATTAATCAGGTTAACGTCAACCCGA
AAGTAGGTCTTACTTTTTCGGCCGCACTTAAATCATTTTTACGTCAGGACCCTGACATTATTATGGTTGGTGAGATTCGT
GACTTAGAAACGGCTGAAATTGCGATTAAAGCTGCGCAAACCGGTCACATGGTAATGTCCACACTGCATACCAATAGTGC
TCCAGAAACATTAACTCGCCTACGTAATATGGGTGTTCCATCCTTTAATATCGCAACTTCTGTCAATCTGGTGATTGCTC
AACGTTTGGCGCGTCGGTTATGCCCACAGTGTAAAATACCTGCTGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACCGAACAAGATCTGACTCATCCCGATTTTCAAGTTTTTCAACCTGTCGGTTGTACTGAGTGTCGGGAAGGCTATAAAGG
TCGAGTAGGTATTTACGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TTGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCAGGATTAAAGAAAGTCATGCAAGGTGTTACTTCC
TTACAAGAAGTCAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

94.737

100

0.947
  pilB Acinetobacter baylyi ADP1

78.07

100

0.781
  pilB Legionella pneumophila strain ERS1305867

51.675

99.474

0.514
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

52.099

91.93

0.479
  pilB Vibrio campbellii strain DS40M4

51.149

91.579

0.468
  pilB Vibrio cholerae strain A1552

50.674

91.053

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

35.761

100

0.388
  pilF Thermus thermophilus HB27

36.655

98.596

0.361

Multiple sequence alignment