Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACM0CX_RS21080 Genome accession   NZ_CP180564
Coordinates   4560928..4562634 (+) Length   568 a.a.
NCBI ID   WP_411637631.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain BJ018     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4555928..4567634
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM0CX_RS21055 pdhR 4557044..4557808 (-) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  ACM0CX_RS21060 ampD 4558084..4558656 (-) 573 WP_324019715.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACM0CX_RS21065 - 4558803..4559273 (+) 471 WP_282464816.1 TIGR02281 family clan AA aspartic protease -
  ACM0CX_RS21070 nadC 4559279..4560136 (+) 858 WP_282464815.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACM0CX_RS21075 - 4560459..4560923 (+) 465 WP_411637630.1 pilin -
  ACM0CX_RS21080 pilB 4560928..4562634 (+) 1707 WP_411637631.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  ACM0CX_RS21085 pilC 4562750..4563991 (+) 1242 WP_411637632.1 type II secretion system F family protein Machinery gene
  ACM0CX_RS21090 pilD 4564014..4564886 (+) 873 WP_331414179.1 A24 family peptidase Machinery gene
  ACM0CX_RS21095 coaE 4564905..4565519 (+) 615 WP_411637633.1 dephospho-CoA kinase -
  ACM0CX_RS21100 zapD 4565557..4566279 (+) 723 WP_411637634.1 cell division protein ZapD -
  ACM0CX_RS21105 yacG 4566289..4566483 (+) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  ACM0CX_RS21110 mutT 4566544..4566957 (-) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62553.92 Da        Isoelectric Point: 5.4342

>NTDB_id=1096654 ACM0CX_RS21080 WP_411637631.1 4560928..4562634(+) (pilB) [Aeromonas hydrophila strain BJ018]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIIAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLTAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLT
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=1096654 ACM0CX_RS21080 WP_411637631.1 4560928..4562634(+) (pilB) [Aeromonas hydrophila strain BJ018]
ATGACCTCTAGCCCCAATAGCGGCCTAGCGTTAAGCTTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAACGCTA
TCTGAGCCAAGCCAAGGCTCAGCGTAAACCCTTTGTGACCTTTCTGATCGAAAATGAAATCCTCGACAGCAAAGCATTGG
CTGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAAAAACATCACGTCCTGCCCATCTACACTCAGGGCCACACCCTCTATATCGCCATGTCAGA
CCCGACCAATGTGTCGGCACTGGAGGATTTTGGTTTCAGCTTCGGGTTGCATACCGAGGCTCTGCTGGTCGAGGAAAACA
AACTCACCACGGCCATCGGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAACTGGAAGTGTCGGATGAAGGGTCACGCCTCGACGAGAGCGTCAATACGGCCGACGATGACGCGCCGATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGAGCCTCCGACCTTCACTTCGAGCCCTATGAGACCAAATATC
GCATCCGCTTTCGGATAGACGGGATCTTGCATGAAATAGCAACTCCGCCAGTCAATCTGGCCAACCGCTTCTCCGCCCGC
CTCAAGGTCATGGCACGGCTCGACATTGCCGAACGGCGACTGCCCCAAGATGGTCGCATCAAACTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGAGTCAATACCCTACCCACCATGTGGGGCGAAAAAATCGTTATCCGTCTGCTCGACTCCTCCG
CCGCACGCCTCAACATTGAGCAGCTTGGTTTTGATGATCGGCAAAAAGCGCAATATCTGCGTGCTCTATCGAAGCCTCAG
GGCATGATCCTGGTAACCGGCCCTACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGCCTCAATATTCTTAATACCAC
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGTGTCAACCAGGTGCAGGTCAATCCCAAAG
CCGGGCTCACCTTCGCCAGCGCCCTGCGCTCTTTCTTGCGCCAGGACCCCGACGTAGTCATGGTGGGTGAAATCCGGGAT
CTGGAAACAGCCGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTATCCACCCTGCACACCAACTCAGCGGC
GGAAACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATTGCTCAAC
GGCTGGCGCGTAAACTGTGCGATCACTGCAAAGCTCCCGAAGTGGTGCCAGAGGCTGAGCTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGACCGCTGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGTTCGGGTGGCTACAAGGGCCGGGT
CGGCATCTACGAGATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAAATTGCCG
CCATCGCCCAGAAGGAGGGCATGCGCACCTTGCGCACCTCCGGCCTTGAGAAGGCCCGCCTGGGGGTGACCAGTCTGACG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

100

0.558
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Legionella pneumophila strain ERS1305867

54.513

99.472

0.542
  pilB Vibrio cholerae strain A1552

54.856

97.887

0.537
  pilB Vibrio campbellii strain DS40M4

52.12

99.648

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

51.779

98.944

0.512
  pilF Neisseria gonorrhoeae MS11

46.099

99.296

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

38.98

96.655

0.377
  pilF Thermus thermophilus HB27

40.152

92.958

0.373

Multiple sequence alignment