Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACMXYH_RS27840 Genome accession   NZ_CP180479
Coordinates   6037778..6039478 (+) Length   566 a.a.
NCBI ID   WP_415765559.1    Uniprot ID   -
Organism   Pseudomonas sp. ZB1P45     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6032778..6044478
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYH_RS27820 - 6032951..6035890 (+) 2940 WP_415765558.1 DUF748 domain-containing protein -
  ACMXYH_RS27825 - 6035890..6036186 (+) 297 WP_129441159.1 DUF2845 domain-containing protein -
  ACMXYH_RS27830 - 6036470..6036823 (-) 354 WP_129441155.1 BON domain-containing protein -
  ACMXYH_RS27835 pilA 6037142..6037552 (-) 411 WP_134422286.1 pilin Machinery gene
  ACMXYH_RS27840 pilB 6037778..6039478 (+) 1701 WP_415765559.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMXYH_RS27845 pilC 6039481..6040698 (+) 1218 WP_415765560.1 type II secretion system F family protein Machinery gene
  ACMXYH_RS27850 pilD 6040700..6041572 (+) 873 WP_415765561.1 prepilin peptidase Machinery gene
  ACMXYH_RS27855 coaE 6041569..6042192 (+) 624 WP_415765562.1 dephospho-CoA kinase -
  ACMXYH_RS27860 yacG 6042189..6042389 (+) 201 WP_134422296.1 DNA gyrase inhibitor YacG -
  ACMXYH_RS27865 - 6042393..6042608 (-) 216 WP_033054332.1 hypothetical protein -
  ACMXYH_RS27870 - 6042672..6043361 (-) 690 WP_415765564.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62869.98 Da        Isoelectric Point: 5.6997

>NTDB_id=1096043 ACMXYH_RS27840 WP_415765559.1 6037778..6039478(+) (pilB) [Pseudomonas sp. ZB1P45]
MNDIALSGLTKQLVLAELLTDKSAQQAYQQAQRDKTPLVTYLVQNKLVKSRLVAEIASEHFGMTLLDLNCLDKETQPKGL
VSEKLIRQHHALPLWRRGNKLFVGVSDPTNHQAINDIQFSTGLNTEAILVEDDKLTDAIEKFFDSPSTGLEDMADVDLDG
VDIESIDDNKQDSIGGQDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTYRVRMRTDGMLREVAKPPIQLANRIASRL
KVMASLDISERRRPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGMDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIQGFNIATSVSLIIAQRLARKLCSHCKKPIEIPREALLKEGFPE
ERIGSFTIYEPVGCDHCNNGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRKDGFNDLRTSGLLKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1096043 ACMXYH_RS27840 WP_415765559.1 6037778..6039478(+) (pilB) [Pseudomonas sp. ZB1P45]
ATGAATGACATCGCCCTTAGCGGTCTGACCAAGCAATTGGTCCTGGCCGAACTGCTCACTGACAAAAGCGCGCAACAGGC
GTATCAGCAAGCCCAACGTGATAAAACCCCGCTAGTCACCTATTTGGTGCAGAACAAGCTGGTGAAAAGCCGCCTGGTCG
CCGAGATCGCGTCGGAACACTTCGGCATGACCTTGCTCGACCTCAACTGCCTGGACAAGGAAACCCAACCCAAGGGTCTG
GTCAGCGAGAAACTGATCCGCCAGCACCACGCCTTGCCCCTCTGGCGACGCGGCAACAAACTGTTCGTGGGCGTTTCCGA
CCCGACCAATCACCAGGCCATCAATGACATCCAGTTCAGCACCGGGCTCAATACCGAAGCCATTCTGGTTGAAGACGACA
AGCTCACCGATGCCATCGAAAAATTCTTCGACAGCCCTAGCACCGGCCTGGAAGACATGGCCGATGTCGACCTTGATGGT
GTGGACATCGAGTCGATCGATGACAACAAACAGGATTCCATTGGCGGACAAGACGCCGACGACGCACCCGTGGTGCGCTT
CGTCCACAAGATGTTGCTCGACGCGATCAAGAGCGGCTCTTCCGACCTGCATTTCGAGCCCTACGAAAAAACCTACCGCG
TGCGGATGCGCACCGACGGCATGCTGCGTGAAGTCGCCAAGCCGCCGATTCAACTGGCCAACCGCATCGCCTCGAGACTG
AAAGTCATGGCCAGTCTCGACATCTCGGAACGGCGTCGGCCCCAGGACGGGCGGATCAAGATGCGCCTGTCCAAGAGCAA
ATCCATAGACTTCCGGGTCAACACCCTGCCGACCCTATGGGGCGAGAAAGTGGTAATCCGGATCCTCGACCCGTCCAGCG
CTCAAATGGGCATCGACGCCCTCGGCTATGAGCCGGACCAGAAAGACCTGTACATGGCCGCCCTCAAGCAGCCACAGGGG
ATGATTCTGGTGACCGGGCCCACCGGTTCGGGTAAAACCGTGTCGCTCTACACCGGGCTGAACATTCTCAATACCGTGGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAACAGG
GAATGGATTTCGCCCAGGCGCTGCGCTCATTTCTGCGACAGGACCCGGACGTGATCATGGTTGGCGAGATTCGTGACCTC
GAAACCGCAGAAATAGCCATCAAGGCCGCCCAGACCGGGCACCTGGTGCTGTCGACCCTGCACACCAACAGCGCCGCCGA
AACCCTGACACGCCTGCACAACATGGGCATCCAGGGGTTCAACATTGCTACCTCGGTCAGCCTGATCATCGCCCAGCGCC
TGGCGCGCAAGCTATGCAGCCATTGCAAGAAACCCATCGAGATTCCCCGGGAGGCGCTGCTCAAGGAAGGCTTCCCCGAG
GAACGGATCGGCTCATTCACGATCTATGAGCCAGTCGGTTGCGATCACTGCAACAACGGTTACAAAGGGCGAGTAGGGAT
TTATGAAGTGGTGAAGAATACCCCGGACCTGCAACGGCTGATCATGGCCGAAGGCAACTCACTGGAAATCGACATCCAGA
TGCGTAAAGACGGCTTCAACGACCTGCGGACGTCGGGCCTGCTCAAAGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.147

99.47

0.588
  pilB Acinetobacter baumannii D1279779

58.688

99.647

0.585
  pilB Legionella pneumophila strain ERS1305867

54.867

99.823

0.548
  pilB Vibrio cholerae strain A1552

50.973

99.823

0.509
  pilF Neisseria gonorrhoeae MS11

50.266

99.47

0.5
  pilB Vibrio campbellii strain DS40M4

48.947

100

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

49.64

98.233

0.488
  pilF Thermus thermophilus HB27

36.219

100

0.362
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.679

93.64

0.362

Multiple sequence alignment