Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACMXD4_RS02710 Genome accession   NZ_CP180476
Coordinates   595162..596877 (+) Length   571 a.a.
NCBI ID   WP_171696693.1    Uniprot ID   -
Organism   Methylomonas sp. TEB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 590162..601877
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXD4_RS02685 (ACMXD4_02685) rpmA 590551..590808 (-) 258 WP_020484554.1 50S ribosomal protein L27 -
  ACMXD4_RS02690 (ACMXD4_02690) rplU 590826..591137 (-) 312 WP_033156113.1 50S ribosomal protein L21 -
  ACMXD4_RS02695 (ACMXD4_02695) ispB 591344..592348 (+) 1005 WP_171696696.1 octaprenyl diphosphate synthase -
  ACMXD4_RS02700 (ACMXD4_02700) - 592353..592757 (-) 405 WP_415879764.1 hypothetical protein -
  ACMXD4_RS02705 (ACMXD4_02705) thiC 593093..594961 (+) 1869 WP_415879765.1 phosphomethylpyrimidine synthase ThiC -
  ACMXD4_RS02710 (ACMXD4_02710) pilB 595162..596877 (+) 1716 WP_171696693.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMXD4_RS02715 (ACMXD4_02715) pilC 596880..598097 (+) 1218 WP_205452935.1 type II secretion system F family protein Machinery gene
  ACMXD4_RS02720 (ACMXD4_02720) pilD 598173..599039 (+) 867 WP_415879766.1 prepilin peptidase Machinery gene
  ACMXD4_RS02725 (ACMXD4_02725) coaE 599043..599654 (+) 612 WP_415879767.1 dephospho-CoA kinase -
  ACMXD4_RS02730 (ACMXD4_02730) zapD 599651..600424 (+) 774 WP_051669750.1 cell division protein ZapD -
  ACMXD4_RS02735 (ACMXD4_02735) yacG 600424..600627 (+) 204 WP_415879768.1 DNA gyrase inhibitor YacG -
  ACMXD4_RS02740 (ACMXD4_02740) - 600644..601588 (-) 945 WP_415879769.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63210.85 Da        Isoelectric Point: 6.1189

>NTDB_id=1095953 ACMXD4_RS02710 WP_171696693.1 595162..596877(+) (pilB) [Methylomonas sp. TEB]
MATAPTNIHFSGLVKCLIQKGLLTEESAATCLQEAQKRKQAFISYLVEHKYADSKTVATLASAEFGVPFFDLDAIDPSFL
PIKLVSEKLIRQHHALPLFKRGNRLFVATADPTNFQALDEIKFHTRHNTENILVEEDKLIRMIDTALEAADTSMKDLLDD
DLDNIDISAGDDSKPADEAIGSDIDDAPIVRFVNKILLDSIKRGVSDIHLEPFEKKFRIRFRADGILSEVASPPVGIANR
IISRLKVMSKMDIAERRVPQDGRIKMQISKNRAIDFRVNTCPTLFGEKVVMRILDPTSAQIGIEKLGFEPDQQKNFLEAI
HKPYGMVLVTGPTGSGKTVSLYTGLNILNSIETNISTAEDPVEITVEGINQVNVNPKAGLTFAEALRAFLRQDPDIIMVG
EIRDLETASIAVKAAQTGHMVLSTLHTNDAPQTINRLVQMGIEPFNIVSAVILIMAQRLARRLCEHCKQEVDYPESTLLS
AGFKQEEIGTFKVYGPVGCEHCNNGYKGRVGIYQVMPITEKMRALILNGGNSMQMAELSKSEGINDLRMSGLLKIKQGVT
SLQEIDRVTKE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1095953 ACMXD4_RS02710 WP_171696693.1 595162..596877(+) (pilB) [Methylomonas sp. TEB]
ATGGCGACAGCACCCACTAACATCCATTTCAGCGGCTTGGTCAAATGCCTGATTCAAAAAGGTTTGTTGACCGAGGAATC
GGCGGCGACCTGTTTGCAGGAGGCGCAGAAGCGTAAACAAGCGTTTATCAGCTATTTGGTGGAACATAAGTATGCGGACA
GCAAAACCGTGGCTACGCTGGCTTCGGCGGAGTTTGGTGTGCCGTTTTTCGATCTGGATGCCATAGACCCTTCATTCTTG
CCGATCAAACTAGTCAGCGAGAAATTGATTCGCCAGCATCATGCCTTACCTTTATTCAAGCGCGGCAACCGCTTGTTTGT
CGCCACCGCCGATCCGACCAATTTTCAGGCGCTGGACGAGATCAAGTTTCATACCCGGCACAATACCGAAAATATTCTGG
TTGAAGAAGACAAGCTGATCCGCATGATAGACACGGCGCTGGAGGCTGCCGATACGTCGATGAAGGATCTGTTGGACGAC
GATCTGGACAATATCGATATTTCCGCAGGCGACGACAGCAAACCGGCCGACGAAGCAATAGGCTCGGATATTGATGACGC
GCCTATCGTCCGTTTTGTAAATAAAATCCTGCTGGACTCGATCAAACGCGGGGTGTCGGACATACACTTGGAACCCTTCG
AGAAAAAATTTCGTATCCGTTTTCGGGCCGACGGTATTTTATCGGAGGTGGCCAGTCCGCCGGTGGGCATTGCCAACCGG
ATTATTTCCCGGCTGAAAGTCATGTCGAAAATGGACATTGCCGAACGGCGGGTGCCGCAGGACGGCCGGATCAAAATGCA
GATTTCCAAGAACCGGGCCATCGATTTTCGGGTAAACACCTGCCCGACGTTGTTCGGTGAAAAAGTGGTAATGCGGATTC
TCGATCCGACCAGTGCGCAAATTGGTATCGAGAAACTCGGTTTCGAACCCGACCAGCAGAAAAATTTTCTGGAGGCGATC
CACAAGCCTTACGGTATGGTGCTGGTCACCGGGCCGACCGGTAGCGGTAAAACCGTATCGCTGTATACCGGGCTGAATAT
TCTGAACAGCATCGAAACCAATATTTCCACCGCCGAAGATCCAGTCGAGATCACCGTGGAAGGCATCAACCAAGTCAATG
TCAATCCCAAGGCCGGCCTGACCTTTGCCGAAGCGCTACGGGCGTTTTTGCGGCAAGACCCTGACATCATCATGGTTGGC
GAGATTCGCGACCTGGAAACCGCCAGTATCGCGGTCAAGGCGGCGCAAACCGGTCACATGGTGTTGTCTACCTTGCATAC
CAATGATGCGCCGCAAACCATCAACCGTTTGGTGCAAATGGGCATAGAACCGTTCAATATTGTTTCGGCGGTGATTTTGA
TCATGGCGCAACGGCTGGCGCGGCGCTTATGCGAGCATTGTAAGCAGGAGGTTGATTATCCGGAAAGCACCTTGCTGTCG
GCCGGGTTTAAGCAGGAGGAAATCGGTACGTTTAAAGTCTACGGTCCAGTGGGTTGTGAACATTGCAATAACGGTTACAA
GGGCAGGGTAGGGATCTACCAGGTAATGCCTATCACTGAAAAAATGCGTGCGCTGATTTTGAACGGCGGTAATTCCATGC
AAATGGCGGAATTGTCGAAAAGTGAAGGAATCAACGATTTAAGAATGTCGGGGCTGTTAAAAATTAAGCAAGGCGTCACT
TCGCTGCAAGAAATTGACCGGGTCACCAAGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.323

100

0.555
  pilB Acinetobacter baumannii D1279779

53.752

100

0.539
  pilB Acinetobacter baylyi ADP1

52.356

100

0.525
  pilF Neisseria gonorrhoeae MS11

48.387

97.723

0.473
  pilB Vibrio parahaemolyticus RIMD 2210633

46.263

98.424

0.455
  pilB Vibrio campbellii strain DS40M4

45.471

98.599

0.448
  pilB Vibrio cholerae strain A1552

49.606

88.967

0.441
  pilF Thermus thermophilus HB27

39.748

97.373

0.387
  pilB/pilB1 Synechocystis sp. PCC 6803

39.344

96.147

0.378
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.64

97.898

0.378

Multiple sequence alignment