Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACMXD4_RS02720 Genome accession   NZ_CP180476
Coordinates   598173..599039 (+) Length   288 a.a.
NCBI ID   WP_415879766.1    Uniprot ID   -
Organism   Methylomonas sp. TEB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 593173..604039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXD4_RS02710 (ACMXD4_02710) pilB 595162..596877 (+) 1716 WP_171696693.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMXD4_RS02715 (ACMXD4_02715) pilC 596880..598097 (+) 1218 WP_205452935.1 type II secretion system F family protein Machinery gene
  ACMXD4_RS02720 (ACMXD4_02720) pilD 598173..599039 (+) 867 WP_415879766.1 prepilin peptidase Machinery gene
  ACMXD4_RS02725 (ACMXD4_02725) coaE 599043..599654 (+) 612 WP_415879767.1 dephospho-CoA kinase -
  ACMXD4_RS02730 (ACMXD4_02730) zapD 599651..600424 (+) 774 WP_051669750.1 cell division protein ZapD -
  ACMXD4_RS02735 (ACMXD4_02735) yacG 600424..600627 (+) 204 WP_415879768.1 DNA gyrase inhibitor YacG -
  ACMXD4_RS02740 (ACMXD4_02740) - 600644..601588 (-) 945 WP_415879769.1 Nudix family hydrolase -
  ACMXD4_RS02745 (ACMXD4_02745) argJ 601601..602815 (-) 1215 WP_415879770.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 288 a.a.        Molecular weight: 31841.06 Da        Isoelectric Point: 7.0510

>NTDB_id=1095955 ACMXD4_RS02720 WP_415879766.1 598173..599039(+) (pilD) [Methylomonas sp. TEB]
MVFATLQHYPLFLISVVCVLGLLVGSFLNVVIYRLPVMMQTGWRRECREYLQLPHDEASEVFNLMLPASHCPSCKTEIKA
YQNIPVISYLLLGGKCAHCGVKIAIRYPLIEAFTGLCSAVVAWHFGYGWALAFALPLTWCLIALSFIDIDQQLLPDSITL
PMLWLGLLLSLFDIYTNSHDAIIGAVAGYLSLWSVYQLFKLLTGKEGMGYGDFKLLALFGAWLGWQYLPLIILLSSLVGA
VIGIAMIVFGKRDAGKPIPFGPYLAMAGWLAMLFGPELNALYLHTAGL

Nucleotide


Download         Length: 867 bp        

>NTDB_id=1095955 ACMXD4_RS02720 WP_415879766.1 598173..599039(+) (pilD) [Methylomonas sp. TEB]
ATGGTTTTTGCCACTCTACAACACTATCCGCTGTTTCTGATCAGCGTCGTTTGCGTGCTGGGCCTTCTGGTCGGCAGCTT
CCTAAACGTGGTTATCTACCGTTTGCCGGTCATGATGCAGACGGGTTGGCGGCGCGAATGCCGGGAATATTTGCAGCTCC
CGCATGATGAAGCAAGCGAAGTGTTCAACCTGATGTTGCCGGCCTCGCATTGCCCGTCGTGCAAAACCGAAATCAAAGCC
TATCAAAACATTCCGGTCATCAGTTACCTGTTGTTGGGCGGCAAATGCGCACATTGTGGGGTGAAAATCGCCATCCGTTA
CCCGCTTATCGAAGCGTTTACCGGTTTATGTTCGGCCGTGGTGGCTTGGCATTTCGGTTACGGTTGGGCTTTAGCGTTTG
CATTACCTCTGACTTGGTGTTTGATCGCATTGAGTTTTATCGACATCGATCAGCAATTATTACCTGATTCGATCACGCTG
CCGATGCTGTGGCTGGGCCTGTTGTTAAGCCTGTTTGATATATACACGAATAGCCACGACGCGATCATCGGCGCGGTGGC
AGGCTATTTGAGCCTGTGGAGCGTTTATCAACTTTTTAAATTGCTGACCGGCAAGGAAGGGATGGGCTACGGCGATTTCA
AGCTGCTGGCACTATTCGGGGCCTGGCTGGGCTGGCAATATCTGCCGCTGATCATTCTTCTGTCATCTTTGGTGGGTGCG
GTCATCGGTATCGCGATGATCGTATTCGGCAAACGCGATGCCGGCAAACCGATACCCTTCGGGCCTTATCTGGCCATGGC
CGGCTGGTTGGCAATGTTGTTCGGGCCGGAGCTGAATGCCTTATATCTGCATACTGCCGGTTTGTAG

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(137-244)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(19-125)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

55.019

93.403

0.514
  pilD Vibrio cholerae strain A1552

52.128

97.917

0.51
  pilD Acinetobacter nosocomialis M2

54.647

93.403

0.51
  pilD Vibrio campbellii strain DS40M4

51.943

98.264

0.51
  pilD Neisseria gonorrhoeae MS11

54.231

90.278

0.49

Multiple sequence alignment