Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACMYNJ_RS13885 Genome accession   NZ_CP180391
Coordinates   3248899..3250029 (-) Length   376 a.a.
NCBI ID   WP_003489921.1    Uniprot ID   A0AAI7ZGP8
Organism   Xanthomonas euvesicatoria pv. polysciadis strain Pgu1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3243899..3255029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMYNJ_RS13855 ruvX 3244416..3244883 (-) 468 WP_277571877.1 Holliday junction resolvase RuvX -
  ACMYNJ_RS13860 - 3244876..3245442 (-) 567 WP_003489930.1 YqgE/AlgH family protein -
  ACMYNJ_RS13865 - 3245719..3246291 (+) 573 WP_005926123.1 DNA-3-methyladenine glycosylase I -
  ACMYNJ_RS13870 - 3246299..3247225 (+) 927 WP_416203186.1 DUF72 domain-containing protein -
  ACMYNJ_RS13875 - 3247518..3247769 (+) 252 WP_416204357.1 hypothetical protein -
  ACMYNJ_RS13880 - 3247805..3248809 (+) 1005 WP_416203187.1 pectate lyase -
  ACMYNJ_RS13885 pilU 3248899..3250029 (-) 1131 WP_003489921.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMYNJ_RS13890 - 3250142..3251165 (-) 1024 Protein_2721 type IV pilus twitching motility protein PilT -
  ACMYNJ_RS13895 - 3251584..3252276 (+) 693 WP_218210793.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMYNJ_RS13900 proC 3252320..3253174 (+) 855 WP_029819894.1 pyrroline-5-carboxylate reductase -
  ACMYNJ_RS13905 - 3253734..3254159 (+) 426 WP_008571830.1 HU family DNA-binding protein -
  ACMYNJ_RS13910 - 3254266..3254907 (+) 642 WP_277574635.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42044.26 Da        Isoelectric Point: 6.8286

>NTDB_id=1095637 ACMYNJ_RS13885 WP_003489921.1 3248899..3250029(-) (pilU) [Xanthomonas euvesicatoria pv. polysciadis strain Pgu1]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLSMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1095637 ACMYNJ_RS13885 WP_003489921.1 3248899..3250029(-) (pilU) [Xanthomonas euvesicatoria pv. polysciadis strain Pgu1]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCCTCGGACCTGTTCATCACCTCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGCCCCATTACCCAGACCCCGCTGACTGCGCAGCAGAGCCGCGACCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGCGTATCCGGG
GTCGGGCGTTTCCGTGTGAGCTGCTTCTACCAGCGTAATCAGGTGGGCATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGACGAGCTGAGCTTGCCGCCGGTGATCAAGACGCTGTCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGAACCGGTAAATCGACTTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAAGATCCGATCGAATTCGTGCACAAGCACGAGGGTTGCATCATCACCCAGCGTGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGTTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCATGCGATCGCATTCGCCGAAACCGGCCATCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAAGATCGCCGCAACCAGCTGTTGATGGACCTGTCGCTCAACCTCAAGGGCGTGGT
CGCGCAGCAGCTGATTCCGACCCCGGACGGCCGCAGCCGTCGCGTGGCGATGGAAATCATGTTGGGCACGCCGCTGGTGC
AGGACTACATACGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCCGGCGAAATCAGTTACGAAGACGCGTTGCGCTACGCCGACTCGCAGAACGA
GGTCCGCCTGCGCATCAAGCTCTCGCAGGGCGGCGATGCCAAGACCTTGTCGCAGGGCCTGGACGGCGTGGAAATTTCCG
AAGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.757

97.606

0.652
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

52.809

94.681

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.457

94.947

0.394
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364
  pilT Acinetobacter nosocomialis M2

39.826

91.489

0.364
  pilT Acinetobacter baumannii D1279779

39.826

91.489

0.364
  pilT Acinetobacter baumannii strain A118

39.826

91.489

0.364

Multiple sequence alignment