Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACMHYC_RS17360 Genome accession   NZ_CP180027
Coordinates   3658420..3660132 (+) Length   570 a.a.
NCBI ID   WP_032875426.1    Uniprot ID   -
Organism   Acinetobacter courvalinii isolate 2024CK-01798     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3653420..3665132
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMHYC_RS17325 (ACMHYC_17325) nusA 3654241..3655725 (-) 1485 WP_004774096.1 transcription termination factor NusA -
  ACMHYC_RS17330 (ACMHYC_17330) rimP 3655763..3656287 (-) 525 WP_199949764.1 ribosome maturation factor RimP -
  ACMHYC_RS17350 (ACMHYC_17350) secG 3656915..3657244 (-) 330 WP_032870579.1 preprotein translocase subunit SecG -
  ACMHYC_RS17355 (ACMHYC_17355) tpiA 3657257..3658048 (-) 792 WP_199949763.1 triose-phosphate isomerase -
  ACMHYC_RS17360 (ACMHYC_17360) pilB 3658420..3660132 (+) 1713 WP_032875426.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMHYC_RS17365 (ACMHYC_17365) pilC 3660157..3661383 (+) 1227 WP_004774089.1 type II secretion system F family protein Machinery gene
  ACMHYC_RS17370 (ACMHYC_17370) pilD 3661389..3662249 (+) 861 WP_199949762.1 prepilin peptidase Machinery gene
  ACMHYC_RS17375 (ACMHYC_17375) coaE 3662251..3662856 (+) 606 WP_199949761.1 dephospho-CoA kinase -
  ACMHYC_RS17380 (ACMHYC_17380) - 3662826..3663764 (-) 939 WP_199949760.1 DMT family transporter -
  ACMHYC_RS17385 (ACMHYC_17385) rlmB 3663796..3664542 (-) 747 WP_004774084.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACMHYC_RS17390 (ACMHYC_17390) - 3664738..3665064 (-) 327 WP_004774082.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63233.59 Da        Isoelectric Point: 5.2266

>NTDB_id=1094104 ACMHYC_RS17360 WP_032875426.1 3658420..3660132(+) (pilB) [Acinetobacter courvalinii isolate 2024CK-01798]
MSGLHTSPKFSGFIRRLVEDSHLSATTMQTAIDTAKKARQDIVAHLIEHHRLSPLTIAETIAAEFGEPLFDLAVYDTALL
PRDLADNKLIQKHRVAPLLQRGQILYVATSNPTNIEALDAIRFNTKLNIEPIIVEHTKLEKLIEQQFGDSGSFDFGDSED
FDLDIDVTQDPAKEEDDTPQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIATPPLQMATRLAS
RLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKAPIDIPKQSLLELGF
TEQDMADPDFKIFQPVGCAECREGYKGRVGVYEVMKVTPEISRIIMEDGNALQIAAASEQAGFNNLRRSGLKKVMQGITS
LQEINRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1094104 ACMHYC_RS17360 WP_032875426.1 3658420..3660132(+) (pilB) [Acinetobacter courvalinii isolate 2024CK-01798]
ATGTCAGGATTACATACGTCTCCAAAGTTTTCGGGGTTTATTCGACGGTTAGTTGAAGACAGTCATCTGTCTGCAACAAC
CATGCAAACAGCGATTGATACAGCTAAAAAAGCACGTCAAGATATCGTTGCACATTTAATTGAACATCATCGTTTATCAC
CACTCACCATTGCTGAAACCATTGCAGCCGAGTTTGGTGAGCCGCTATTTGATCTGGCCGTGTACGATACCGCCCTGTTA
CCGCGTGATCTGGCTGACAACAAACTGATTCAGAAACATCGAGTCGCGCCCCTGCTGCAGCGTGGTCAGATTTTATACGT
TGCCACCAGCAATCCAACCAATATTGAAGCGCTGGACGCCATTCGCTTTAACACCAAGCTCAATATCGAACCGATCATTG
TTGAGCACACCAAACTCGAAAAACTGATTGAACAGCAATTTGGCGATTCAGGCAGTTTCGATTTTGGCGATAGTGAAGAT
TTTGATCTGGATATTGATGTAACTCAGGATCCAGCCAAAGAAGAAGACGATACGCCACAAGGTGATGAGTCCCCGATTGT
TAAATATATCAATAAATTATTAATCGATGCGATTCGTATGGGTGCATCAGATTTACATTTTGAACCCTATGAAAAGATGT
ATCGGGTGCGTTACCGCGTCGATGGGGTGCTGCGTCAAATTGCCACGCCACCCTTACAAATGGCAACGCGATTAGCTTCT
CGCTTGAAAGTCATGTCGCAAATGGACATTTCAGAGAAACGGATGCCGCAAGATGGCCGTATTAAATTAAAGCTATCCAA
AACCAAAGCCATCGACTTCCGTGTCAACTCACTGCCGACGCTGTTTGGCGAGAAGCTGGTGCTGCGTATTCTTGATCCAT
CCAGTGCTATGCTCGGGATTGAAGCGCTGGGTTACGAAGACGAGCAAAAAGCCTTATTTATGGAAGCGCTGGATAAGCCT
CAAGGCATGCTGCTGATTACCGGCCCAACTGGTTCCGGTAAAACCGTGTCTCTGTATACCGGTTTGAATATTCTCAATAC
TGAAGATACCAATATTTCTACGGCCGAAGATCCGGTCGAAATCAATTTGGAAGGCATTAATCAGGTCAACGTCAATCCTA
AAACTGGACTGACCTTCTCTGCTGCATTGAAAGCATTCTTACGTCAGGATCCGGACATCATCATGGTCGGTGAGATCCGG
GATCTGGAAACCGCAGAAATTGCCATTAAAGCCGCTCAAACCGGTCATATGGTGATGTCGACACTGCATACCAACAGTGC
CCCTGAAACCTTGACCCGTTTGCGCAATATGGGTGTGCCGTCCTTCAACATTGCCACTTCGGTCAATCTGGTGATTGCAC
AACGTCTAGCACGTCGCTTATGCTCGCAATGTAAGGCACCCATTGATATTCCAAAACAAAGCCTGTTAGAGCTTGGCTTT
ACTGAGCAGGATATGGCCGATCCCGATTTCAAGATTTTTCAACCGGTGGGCTGTGCCGAATGTCGCGAAGGTTATAAAGG
CCGTGTCGGCGTCTATGAAGTCATGAAAGTTACGCCAGAAATTTCCAGAATTATTATGGAAGACGGCAATGCCTTACAGA
TTGCAGCGGCATCCGAACAAGCAGGTTTTAATAATTTACGCCGTTCAGGTTTAAAAAAGGTGATGCAGGGAATTACCTCT
TTGCAAGAAATTAACCGCGTGACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

83.713

100

0.839
  pilB Acinetobacter baylyi ADP1

77.487

100

0.779
  pilB Legionella pneumophila strain ERS1305867

53.42

94.912

0.507
  pilF Neisseria gonorrhoeae MS11

49.556

98.772

0.489
  pilB Vibrio cholerae strain A1552

48.551

96.842

0.47
  pilB Vibrio parahaemolyticus RIMD 2210633

51.737

90.877

0.47
  pilB Vibrio campbellii strain DS40M4

50.579

90.877

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

33.876

100

0.365
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.171

93.158

0.365
  pilF Thermus thermophilus HB27

39.171

93.158

0.365

Multiple sequence alignment