Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACMG4N_RS15050 Genome accession   NZ_CP179932
Coordinates   3275385..3277094 (+) Length   569 a.a.
NCBI ID   WP_297767104.1    Uniprot ID   -
Organism   Alcanivorax sp. IL2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3270385..3282094
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMG4N_RS15035 (ACMG4N_15035) - 3271936..3273273 (+) 1338 WP_297767100.1 alanine/glycine:cation symporter family protein -
  ACMG4N_RS15040 (ACMG4N_15040) nadC 3273344..3274186 (+) 843 WP_297767101.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACMG4N_RS15045 (ACMG4N_15045) - 3274455..3274931 (-) 477 WP_297767103.1 pilin -
  ACMG4N_RS15050 (ACMG4N_15050) pilB 3275385..3277094 (+) 1710 WP_297767104.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMG4N_RS15055 (ACMG4N_15055) pilC 3277097..3278323 (+) 1227 WP_414432267.1 type II secretion system F family protein Machinery gene
  ACMG4N_RS15060 (ACMG4N_15060) pilD 3278327..3279205 (+) 879 WP_414432269.1 prepilin peptidase Machinery gene
  ACMG4N_RS15065 (ACMG4N_15065) coaE 3279324..3279923 (+) 600 WP_297767110.1 dephospho-CoA kinase -
  ACMG4N_RS15070 (ACMG4N_15070) - 3279926..3280117 (+) 192 WP_035249304.1 DNA gyrase inhibitor YacG -
  ACMG4N_RS15075 (ACMG4N_15075) - 3280247..3281203 (-) 957 WP_414432271.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62909.72 Da        Isoelectric Point: 5.1681

>NTDB_id=1093964 ACMG4N_RS15050 WP_297767104.1 3275385..3277094(+) (pilB) [Alcanivorax sp. IL2]
MTSQTLSFSGLPQRLIRQELLTAEEIQQAQKQAREERMSLVYQLVTSGKLSANVVAGAVAEEFGDPILDLSTFQRDSLVK
DLVDPKLITKHHVLPLFRRGSRLFVAVADPTNLPALEEIRFNTGLNVETIIVEQDKLTHWIDNLESDSEDSAFDDLDDGL
DDLDIGAAEEKTTDETKSGSDDAPIVRFVNKLLLDAIKGGASDLHFEPYEKTYRVRFRQDGILKTVSKPPVNIASKLSAR
LKVMSRLDISERRVPQDGRIKLKISKTRAIDFRVNTCPTLFGEKIVLRILDPESAKMGIDALGYEENQKQLYMDALQKPQ
GMILVTGPTGSGKTVSLYTGVNILNTPERNISTAEDPVEINLEGINQVNVNPKQGMDFAAALRAFLRQDPDVILVGEIRD
LETANIAIKAAQTGHLVLSTLHTNSAPETLTRLRNMGVPSFNIATSVVLIIAQRLARRLCEHCKTKVEIPKQSLLEMGFT
EEDAASEFTVYGPEGCDKCNNGYKGRVGIYEVVKITDGIARIIMEEGNSIQIGDQCRKEGFNDLHRSGLVKAMQGVTSLE
EVNRVTSGH

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=1093964 ACMG4N_RS15050 WP_297767104.1 3275385..3277094(+) (pilB) [Alcanivorax sp. IL2]
ATGACTAGCCAGACCCTTTCATTCAGCGGCTTACCGCAGCGCCTGATTCGGCAGGAGCTACTAACCGCCGAGGAAATACA
GCAGGCGCAAAAACAGGCCCGTGAAGAACGGATGAGCCTGGTTTACCAGCTCGTCACTTCGGGCAAGCTGTCGGCCAACG
TTGTGGCCGGAGCCGTTGCCGAAGAGTTCGGAGACCCGATCCTGGATCTCAGCACCTTCCAGCGGGACAGCCTGGTCAAA
GATCTTGTCGACCCCAAACTGATTACCAAGCACCATGTGCTGCCATTGTTTCGCCGGGGCAGCCGTTTATTTGTGGCCGT
TGCCGACCCTACCAACTTGCCCGCTCTGGAAGAAATCCGCTTCAATACAGGCCTCAACGTTGAAACCATTATTGTTGAGC
AAGACAAGCTGACCCACTGGATCGATAACCTTGAGTCCGATTCAGAAGACAGCGCTTTTGATGACCTGGATGATGGGCTG
GATGACCTTGATATAGGTGCGGCAGAGGAAAAAACTACCGACGAGACGAAATCCGGCTCAGATGATGCCCCCATTGTTCG
CTTCGTCAACAAGCTCCTGCTGGATGCCATCAAAGGCGGAGCTTCGGATCTTCATTTCGAGCCATATGAGAAAACTTATC
GAGTACGATTCCGCCAAGACGGGATTCTCAAAACAGTCTCCAAACCGCCGGTCAATATTGCCAGTAAGCTTTCAGCTCGC
CTTAAAGTCATGTCGCGGCTGGACATCTCGGAACGGAGGGTCCCCCAGGATGGACGCATCAAACTTAAAATCTCCAAGAC
CCGAGCAATCGATTTTCGAGTGAACACCTGCCCTACTCTGTTTGGGGAAAAGATCGTCCTGCGGATCCTCGACCCTGAAT
CGGCCAAGATGGGCATCGACGCCCTCGGCTACGAAGAAAATCAGAAACAGCTCTATATGGATGCCTTGCAAAAACCCCAG
GGCATGATTCTGGTTACAGGCCCCACCGGCTCCGGGAAAACCGTCTCCCTTTATACCGGGGTAAACATTCTCAACACGCC
CGAGCGGAACATTTCCACTGCCGAAGACCCAGTAGAGATCAACCTGGAGGGCATCAACCAGGTGAACGTCAACCCCAAAC
AGGGCATGGACTTCGCTGCCGCCTTGCGCGCTTTCCTCCGTCAGGACCCTGACGTCATTCTGGTTGGTGAGATCCGCGAT
CTGGAAACCGCCAATATTGCAATCAAGGCAGCACAAACCGGTCACTTGGTACTGTCGACCCTCCACACCAACAGCGCACC
GGAAACGCTGACCCGTCTTCGTAACATGGGTGTCCCGTCCTTTAATATCGCCACCTCCGTGGTATTGATTATCGCCCAGC
GTTTGGCTCGCAGGCTTTGCGAACACTGCAAAACCAAGGTGGAAATCCCAAAACAATCGCTGCTTGAGATGGGATTCACG
GAAGAAGATGCGGCTTCCGAGTTTACTGTGTACGGCCCTGAGGGATGCGACAAATGCAACAACGGTTACAAAGGGCGCGT
AGGGATCTACGAAGTGGTCAAGATCACTGATGGTATTGCTCGAATTATTATGGAAGAGGGCAACTCCATTCAGATTGGTG
ACCAATGCCGCAAGGAAGGATTCAATGATCTACACCGCTCCGGGCTGGTGAAAGCCATGCAAGGCGTAACCAGCCTGGAA
GAAGTTAACCGTGTAACAAGCGGACATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-149)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

62.1

98.77

0.613
  pilB Acinetobacter baylyi ADP1

61.21

98.77

0.605
  pilB Legionella pneumophila strain ERS1305867

54.291

100

0.545
  pilB Vibrio parahaemolyticus RIMD 2210633

51.526

97.891

0.504
  pilB Vibrio campbellii strain DS40M4

50.628

97.891

0.496
  pilB Vibrio cholerae strain A1552

50.54

97.715

0.494
  pilF Neisseria gonorrhoeae MS11

50.179

98.067

0.492
  pilB/pilB1 Synechocystis sp. PCC 6803

37.745

100

0.406
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.057

93.146

0.364
  pilF Thermus thermophilus HB27

37.23

97.715

0.364

Multiple sequence alignment