Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACMG4N_RS15060 Genome accession   NZ_CP179932
Coordinates   3278327..3279205 (+) Length   292 a.a.
NCBI ID   WP_414432269.1    Uniprot ID   -
Organism   Alcanivorax sp. IL2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3273327..3284205
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMG4N_RS15040 (ACMG4N_15040) nadC 3273344..3274186 (+) 843 WP_297767101.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACMG4N_RS15045 (ACMG4N_15045) - 3274455..3274931 (-) 477 WP_297767103.1 pilin -
  ACMG4N_RS15050 (ACMG4N_15050) pilB 3275385..3277094 (+) 1710 WP_297767104.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMG4N_RS15055 (ACMG4N_15055) pilC 3277097..3278323 (+) 1227 WP_414432267.1 type II secretion system F family protein Machinery gene
  ACMG4N_RS15060 (ACMG4N_15060) pilD 3278327..3279205 (+) 879 WP_414432269.1 prepilin peptidase Machinery gene
  ACMG4N_RS15065 (ACMG4N_15065) coaE 3279324..3279923 (+) 600 WP_297767110.1 dephospho-CoA kinase -
  ACMG4N_RS15070 (ACMG4N_15070) - 3279926..3280117 (+) 192 WP_035249304.1 DNA gyrase inhibitor YacG -
  ACMG4N_RS15075 (ACMG4N_15075) - 3280247..3281203 (-) 957 WP_414432271.1 Nudix family hydrolase -
  ACMG4N_RS15080 (ACMG4N_15080) argJ 3281343..3282545 (-) 1203 WP_414432273.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 292 a.a.        Molecular weight: 31908.82 Da        Isoelectric Point: 5.7734

>NTDB_id=1093966 ACMG4N_RS15060 WP_414432269.1 3278327..3279205(+) (pilD) [Alcanivorax sp. IL2]
MIDSFLNILSASPTLLVGLCVFLGLLIGSFLNVVIYRVPVMMERGWKQEAREILELPAEDDGEIFNLVTPRSRCPKCDHA
ITWYENIPVVSWLALKGKCKGCATPISKRYPIIEVASGVLAGLCAWHFGYGPWLTFTLFATWTLLAAAMIDADTTLLPDS
MNYPLLWAGLLAALLGVSPVSLPDAVVGAMAGYLSLWSVYWLFKLLTGKEGMGYGDFKLLAALGAWTGWQYLPLIILLSS
VVGLVFTLIATLFGGEKRTQIPFGPYLAAAGWIALLWGDTIVSSYLGMFRIS

Nucleotide


Download         Length: 879 bp        

>NTDB_id=1093966 ACMG4N_RS15060 WP_414432269.1 3278327..3279205(+) (pilD) [Alcanivorax sp. IL2]
ATGATCGACAGTTTTCTGAATATCCTCTCCGCGAGCCCAACCCTGCTGGTTGGGCTCTGTGTTTTTCTGGGCCTGTTAAT
TGGCAGCTTCCTCAATGTCGTCATCTACCGCGTACCGGTGATGATGGAGCGTGGCTGGAAACAGGAAGCCCGCGAAATTC
TCGAGCTACCCGCAGAGGATGATGGCGAGATCTTCAACCTGGTGACGCCGCGTTCGCGCTGTCCAAAATGTGACCACGCC
ATCACCTGGTATGAAAATATTCCGGTGGTGAGCTGGTTAGCGCTGAAAGGCAAATGCAAAGGCTGCGCAACACCGATCAG
CAAGCGTTATCCGATCATTGAAGTTGCCAGTGGCGTGCTGGCCGGCCTGTGCGCCTGGCACTTCGGTTATGGTCCCTGGC
TGACTTTTACCCTGTTTGCCACCTGGACGCTGCTGGCAGCCGCAATGATTGATGCGGATACCACCCTGCTACCGGACAGC
ATGAATTACCCTCTGCTGTGGGCCGGCCTGTTGGCGGCACTGCTTGGCGTTTCACCGGTTTCCCTGCCGGATGCCGTGGT
GGGTGCCATGGCAGGTTATCTGTCCTTGTGGAGCGTGTACTGGCTGTTCAAGCTGCTTACTGGCAAGGAAGGCATGGGCT
ATGGTGACTTCAAGCTTCTTGCTGCCCTGGGCGCCTGGACGGGTTGGCAGTATCTGCCGCTGATTATTTTGCTGTCTTCC
GTGGTGGGCCTGGTGTTCACCCTCATCGCCACTCTCTTCGGTGGCGAAAAGCGCACCCAGATCCCTTTTGGCCCCTATCT
GGCGGCCGCTGGCTGGATTGCATTGCTGTGGGGCGATACCATCGTCAGCTCGTATCTGGGGATGTTCAGAATCAGTTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(139-248)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(23-128)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

56.115

95.205

0.534
  pilD Vibrio campbellii strain DS40M4

53.357

96.918

0.517
  pilD Neisseria gonorrhoeae MS11

51.636

94.178

0.486
  pilD Acinetobacter baumannii D1279779

49.638

94.521

0.469
  pilD Acinetobacter nosocomialis M2

49.275

94.521

0.466

Multiple sequence alignment