Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACLSSQ_RS15410 Genome accession   NZ_CP179682
Coordinates   3236429..3237673 (+) Length   414 a.a.
NCBI ID   WP_014236420.1    Uniprot ID   A0A5H2YT80
Organism   Azospira sp. APE16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3224203..3237673 3236429..3237673 within 0


Gene organization within MGE regions


Location: 3224203..3237673
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLSSQ_RS15345 (ACLSSQ_15345) - 3224203..3224703 (-) 501 WP_416244298.1 DUF6776 family protein -
  ACLSSQ_RS15350 (ACLSSQ_15350) rpsI 3225147..3225539 (-) 393 WP_014236432.1 30S ribosomal protein S9 -
  ACLSSQ_RS15355 (ACLSSQ_15355) rplM 3225549..3225977 (-) 429 WP_014236431.1 50S ribosomal protein L13 -
  ACLSSQ_RS15360 (ACLSSQ_15360) - 3226478..3226894 (+) 417 WP_130459322.1 OsmC family protein -
  ACLSSQ_RS15365 (ACLSSQ_15365) coq7 3226932..3227555 (-) 624 WP_152090511.1 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase -
  ACLSSQ_RS15370 (ACLSSQ_15370) - 3227577..3228137 (-) 561 WP_232058806.1 CNP1-like family protein -
  ACLSSQ_RS15375 (ACLSSQ_15375) - 3228194..3228805 (-) 612 WP_014236427.1 CBS domain-containing protein -
  ACLSSQ_RS15380 (ACLSSQ_15380) - 3228860..3229384 (-) 525 WP_014236426.1 RNA pyrophosphohydrolase -
  ACLSSQ_RS15385 (ACLSSQ_15385) - 3229544..3231256 (+) 1713 WP_416243166.1 proline--tRNA ligase -
  ACLSSQ_RS15390 (ACLSSQ_15390) - 3231244..3231852 (+) 609 WP_130459324.1 lytic transglycosylase domain-containing protein -
  ACLSSQ_RS15395 (ACLSSQ_15395) ffh 3232170..3233525 (-) 1356 WP_130459325.1 signal recognition particle protein -
  ACLSSQ_RS15400 (ACLSSQ_15400) - 3233571..3234410 (+) 840 WP_416243167.1 cytochrome C assembly family protein -
  ACLSSQ_RS15405 (ACLSSQ_15405) pilB 3234686..3236404 (+) 1719 WP_014236421.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLSSQ_RS15410 (ACLSSQ_15410) pilC 3236429..3237673 (+) 1245 WP_014236420.1 type II secretion system F family protein Machinery gene

Sequence

Protein


Download         Length: 414 a.a.        Molecular weight: 45010.15 Da        Isoelectric Point: 9.8402

>NTDB_id=1092566 ACLSSQ_RS15410 WP_014236420.1 3236429..3237673(+) (pilC) [Azospira sp. APE16]
MATAGKNTQNTKRADPSIKEFVFVWEGKDKTGKIIRGELRATSETVVQATLRRQGVLVTKVSKQRFKSGGKITDKDITLF
TRQLATMMKSGVPLLQAFDIVGKGHSNPAVGKLLLDIKADVETGSALNQAFRKYPLYFDSLFCNLIGAGEQAGILDTLLD
RLAIYKEKILAVKSKIKSALFYPIAVIVVAFCITAVIMIFVIPAFKEVFKSFGADLPAPTLVVIAISDFFVAYWYLIFGI
LGGGIYSFLESWKRSEAVQIAMDKALLRMPIFGPLIRKSVIARWTRTLATMFAAGVPLVEALDSVGGASGNHVYKMATRQ
IQSEVSTGTSLTNSMQNVNLFPNMVIQMVAIGEESGALDSMLSKVADFFEAEVDDSVAALSSLMEPIIMVILGVLIGGMV
VAMYLPIFKMGSVV

Nucleotide


Download         Length: 1245 bp        

>NTDB_id=1092566 ACLSSQ_RS15410 WP_014236420.1 3236429..3237673(+) (pilC) [Azospira sp. APE16]
ATGGCCACTGCCGGCAAAAACACACAAAACACCAAGCGTGCCGACCCAAGCATCAAGGAGTTCGTCTTTGTTTGGGAAGG
CAAGGACAAGACCGGGAAAATCATCCGGGGGGAATTGCGCGCCACCAGCGAAACGGTGGTCCAGGCAACCCTGCGGCGCC
AAGGCGTGCTGGTTACCAAGGTATCCAAGCAGCGTTTCAAGAGTGGCGGCAAGATCACCGACAAGGACATCACCCTGTTC
ACCCGGCAGCTGGCCACCATGATGAAATCTGGGGTGCCGCTACTCCAGGCCTTCGACATCGTTGGCAAAGGCCACTCAAA
CCCCGCTGTCGGGAAACTGCTGCTGGATATCAAGGCTGATGTCGAAACGGGTAGCGCTCTGAACCAAGCTTTCCGCAAGT
ACCCACTTTACTTCGACAGCCTATTCTGCAACTTGATCGGCGCCGGCGAGCAGGCCGGCATCCTCGATACACTCCTTGAT
CGACTCGCCATCTACAAGGAAAAAATCCTCGCCGTCAAAAGCAAGATCAAGTCAGCCCTGTTCTATCCAATTGCCGTCAT
TGTCGTAGCTTTCTGCATTACGGCTGTGATCATGATTTTTGTAATTCCTGCGTTCAAAGAGGTTTTCAAATCGTTTGGTG
CAGACCTTCCTGCCCCGACACTAGTCGTAATAGCGATTTCTGACTTCTTCGTTGCCTATTGGTATTTGATCTTCGGCATA
TTGGGAGGAGGAATCTATTCCTTCCTGGAGTCGTGGAAGCGCTCCGAGGCCGTTCAGATTGCCATGGACAAGGCATTGCT
GAGGATGCCGATTTTCGGCCCTCTCATACGGAAATCGGTTATCGCCAGATGGACGAGAACCCTGGCAACCATGTTTGCCG
CCGGCGTTCCTCTGGTTGAGGCATTGGACTCAGTGGGCGGAGCCTCAGGCAACCACGTTTACAAAATGGCAACGCGTCAG
ATCCAAAGCGAAGTCAGTACCGGCACCAGCCTGACCAACTCAATGCAGAATGTGAACCTGTTCCCCAATATGGTGATCCA
GATGGTTGCCATCGGTGAGGAATCTGGGGCCCTCGACTCAATGCTGTCCAAGGTTGCGGACTTTTTTGAAGCCGAAGTGG
ACGATTCTGTTGCGGCCCTCTCCAGCCTGATGGAGCCAATCATCATGGTCATATTGGGGGTTCTCATCGGCGGCATGGTG
GTGGCGATGTACCTGCCGATCTTCAAAATGGGCTCCGTGGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(80-203)

Type II secretion system (T2SS), protein F (T2SSF)

(284-406)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5H2YT80

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

55.808

95.652

0.534
  pilC Pseudomonas stutzeri DSM 10701

53.713

97.585

0.524
  pilC Acinetobacter baumannii D1279779

53.383

96.377

0.514
  pilC Acinetobacter baylyi ADP1

52.01

96.135

0.5
  pilG Neisseria gonorrhoeae MS11

51.889

95.894

0.498
  pilG Neisseria meningitidis 44/76-A

51.637

95.894

0.495
  pilC Vibrio campbellii strain DS40M4

38.725

98.551

0.382
  pilC Vibrio cholerae strain A1552

38.636

95.652

0.37

Multiple sequence alignment