Detailed information NTG1092565

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: ACLSSQ_RS15405
• Genome accession: NZ_CP179682
• Coordinates: 3234686..3236404 (+)
• Length: 572 a.a.
• NCBI ID: WP_014236421.1
• Uniprot ID: G8QME2
• Organism: Azospira sp. APE16
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3224203..3237673	3234686..3236404	within	0

Gene organization within MGE regions

Location: 3224203..3237673

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLSSQ_RS15345 (ACLSSQ_15345)	-	3224203..3224703 (-)	501	WP_416244298.1	DUF6776 family protein	-
ACLSSQ_RS15350 (ACLSSQ_15350)	rpsI	3225147..3225539 (-)	393	WP_014236432.1	30S ribosomal protein S9	-
ACLSSQ_RS15355 (ACLSSQ_15355)	rplM	3225549..3225977 (-)	429	WP_014236431.1	50S ribosomal protein L13	-
ACLSSQ_RS15360 (ACLSSQ_15360)	-	3226478..3226894 (+)	417	WP_130459322.1	OsmC family protein	-
ACLSSQ_RS15365 (ACLSSQ_15365)	coq7	3226932..3227555 (-)	624	WP_152090511.1	2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase	-
ACLSSQ_RS15370 (ACLSSQ_15370)	-	3227577..3228137 (-)	561	WP_232058806.1	CNP1-like family protein	-
ACLSSQ_RS15375 (ACLSSQ_15375)	-	3228194..3228805 (-)	612	WP_014236427.1	CBS domain-containing protein	-
ACLSSQ_RS15380 (ACLSSQ_15380)	-	3228860..3229384 (-)	525	WP_014236426.1	RNA pyrophosphohydrolase	-
ACLSSQ_RS15385 (ACLSSQ_15385)	-	3229544..3231256 (+)	1713	WP_416243166.1	proline--tRNA ligase	-
ACLSSQ_RS15390 (ACLSSQ_15390)	-	3231244..3231852 (+)	609	WP_130459324.1	lytic transglycosylase domain-containing protein	-
ACLSSQ_RS15395 (ACLSSQ_15395)	ffh	3232170..3233525 (-)	1356	WP_130459325.1	signal recognition particle protein	-
ACLSSQ_RS15400 (ACLSSQ_15400)	-	3233571..3234410 (+)	840	WP_416243167.1	cytochrome C assembly family protein	-
ACLSSQ_RS15405 (ACLSSQ_15405)	pilB	3234686..3236404 (+)	1719	WP_014236421.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ACLSSQ_RS15410 (ACLSSQ_15410)	pilC	3236429..3237673 (+)	1245	WP_014236420.1	type II secretion system F family protein	Machinery gene

Sequence

Protein

• Length: 572 a.a.
• Molecular weight: 62241.46 Da
• Isoelectric Point: 5.3501

>NTDB_id=1092565 ACLSSQ_RS15405 WP_014236421.1 3234686..3236404(+) (pilB) [Azospira sp. APE16]
MAATFQVPLSGLARAMVQAGRLKEAEAEAFMAQASEKGTTFIEQVIAGKRASAKEIAVFAGETFGYPVLDLSAFDEANIP
ASAIDRKLIATHRVIPLNKRGNRLSVAIADPTNLRALDEIRFQTGLAVDSVVVEEPKLAPLVVKYSESAEENLKNLTNED
LNLEFAEEEDQAKADEAVSLDVDDAPVVKFIQKILLDAINDGASDIHFEPYEKFYRIRYRIDGELREVANPPLAIKEKVA
SRIKVISRMDISEKRVPQDGRMKLVLSKTRAIDFRVSTLPTLHGEKIVMRILDPSSATLGIEALGYEPEQKEALLNAIHR
PYGMVLVTGPTGSGKTVSLYTCLNILNKPGINISTAEDPAEINLAGINQVNVNDKAGLTFSAALKSFLRQDPDIIMVGEI
RDLDTAEIAIKAAQTGHMVLSTLHTNDAPTTLERLKNMGVPTFNIASSVILITAQRLARRLCNCKVPLHVPTETLLNAGF
TEAELDGSWTLYGPNPEGCERCKGGGFKGRVGIYQVMPVSEEIQRIIMANGNSMDIAAQAKKEGVKDLRQSGLLKVKQGV
TSLDEVLSTTNA

Nucleotide

• Length: 1719 bp

>NTDB_id=1092565 ACLSSQ_RS15405 WP_014236421.1 3234686..3236404(+) (pilB) [Azospira sp. APE16]
ATGGCAGCTACTTTTCAGGTTCCCCTAAGTGGTCTTGCGCGCGCCATGGTCCAGGCTGGACGCCTCAAAGAGGCCGAAGC
CGAAGCCTTCATGGCCCAAGCCAGCGAAAAGGGCACGACATTCATTGAGCAGGTGATCGCCGGCAAGCGGGCCAGCGCCA
AGGAAATAGCCGTATTCGCAGGAGAAACGTTCGGCTACCCGGTCCTTGATCTGAGCGCTTTCGATGAAGCCAACATTCCG
GCCAGCGCCATTGACCGGAAGCTCATCGCCACCCACCGCGTCATACCGCTGAACAAGCGCGGCAACCGGCTCTCGGTAGC
GATAGCTGACCCAACAAATTTGCGGGCCTTGGACGAAATTCGCTTCCAGACAGGCTTGGCAGTGGACTCCGTCGTTGTCG
AGGAGCCCAAACTGGCACCGCTGGTCGTCAAATATTCGGAATCGGCAGAAGAAAACCTCAAGAATCTCACCAATGAGGAC
TTGAACCTCGAATTTGCGGAAGAAGAAGATCAGGCCAAGGCCGATGAAGCCGTATCCCTGGATGTGGATGACGCACCGGT
CGTCAAGTTCATTCAGAAAATACTGCTGGATGCGATCAACGATGGCGCTTCCGACATCCACTTCGAGCCGTATGAAAAGT
TTTACCGCATTCGCTATCGCATCGACGGCGAGTTGCGAGAGGTTGCCAATCCGCCCCTGGCAATCAAGGAAAAGGTAGCT
TCCCGCATCAAGGTCATTTCCCGGATGGACATTTCCGAGAAGCGGGTCCCGCAAGACGGCCGGATGAAGCTGGTCCTTTC
CAAAACCAGAGCCATCGACTTCCGGGTCAGCACGCTACCAACGCTGCATGGCGAAAAGATCGTCATGCGTATTCTGGATC
CGTCCTCCGCCACGCTGGGCATCGAGGCGCTGGGCTACGAGCCGGAACAGAAGGAAGCCCTGCTCAACGCCATCCACCGT
CCTTATGGCATGGTTCTGGTAACCGGCCCGACCGGTTCCGGCAAAACCGTATCCTTGTACACCTGCCTCAACATCCTGAA
CAAGCCTGGCATCAACATCTCCACCGCGGAAGACCCGGCAGAAATCAACCTGGCCGGCATCAACCAGGTAAACGTCAACG
ACAAGGCGGGCCTGACCTTTTCTGCCGCCCTCAAGTCCTTCCTGCGGCAGGATCCGGACATCATCATGGTGGGTGAAATC
CGCGACCTGGACACTGCCGAAATCGCAATCAAGGCGGCCCAGACCGGCCACATGGTGCTATCCACCCTGCACACCAACGA
TGCCCCGACGACACTGGAGCGCCTGAAGAACATGGGCGTCCCCACCTTCAACATTGCCTCGTCCGTCATCCTGATCACCG
CCCAGCGGCTGGCCCGCCGTCTGTGCAACTGCAAGGTCCCGCTCCACGTACCCACCGAAACCCTTCTGAATGCAGGCTTT
ACCGAAGCAGAACTGGATGGCAGTTGGACACTCTATGGCCCCAACCCGGAAGGATGCGAGCGCTGCAAGGGAGGCGGTTT
CAAGGGCCGGGTAGGCATTTACCAGGTGATGCCGGTTTCCGAGGAAATTCAGCGCATCATCATGGCCAATGGCAACTCCA
TGGATATTGCTGCCCAGGCCAAAAAGGAAGGGGTCAAGGACTTGCGCCAGTCCGGCCTGTTGAAAGTAAAGCAGGGAGTC
ACCTCCCTGGACGAAGTGCTGTCTACGACAAACGCCTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (52-151)

• Type II/IV secretion system protein (T2SSE) (193-460)

3D structure

• AlphaFold DB: G8QME2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	55.401	100	0.556
pilB	Acinetobacter baylyi ADP1	54.939	100	0.554
pilB	Legionella pneumophila strain ERS1305867	53.322	100	0.533
pilF	Neisseria gonorrhoeae MS11	53.086	99.126	0.526
pilB	Vibrio cholerae strain A1552	49.558	98.776	0.49
pilB	Vibrio parahaemolyticus RIMD 2210633	47.687	98.252	0.469
pilB	Vibrio campbellii strain DS40M4	47.08	98.776	0.465
pilF	Thermus thermophilus HB27	38.489	99.476	0.383