Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACLKMI_RS10360 Genome accession   NZ_CP178625
Coordinates   2173439..2174668 (+) Length   409 a.a.
NCBI ID   WP_413400821.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. KJ71-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2168439..2179668
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKMI_RS10340 (ACLKMI_10340) ampE 2169404..2170255 (-) 852 WP_413400817.1 beta-lactamase regulator AmpE -
  ACLKMI_RS10345 (ACLKMI_10345) ampD 2170543..2171088 (-) 546 WP_413400818.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACLKMI_RS10350 (ACLKMI_10350) nadC 2171488..2172333 (+) 846 WP_413400819.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACLKMI_RS10355 (ACLKMI_10355) - 2172884..2173345 (+) 462 WP_413400820.1 pilin -
  ACLKMI_RS10360 (ACLKMI_10360) pilC 2173439..2174668 (+) 1230 WP_413400821.1 type II secretion system F family protein Machinery gene
  ACLKMI_RS10365 (ACLKMI_10365) pilD 2175305..2176216 (+) 912 WP_413400822.1 prepilin peptidase Machinery gene
  ACLKMI_RS10370 (ACLKMI_10370) coaE 2176219..2176848 (+) 630 WP_413400823.1 dephospho-CoA kinase -
  ACLKMI_RS10375 (ACLKMI_10375) pilB 2176984..2178660 (+) 1677 WP_413400824.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLKMI_RS10380 (ACLKMI_10380) yacG 2178726..2178956 (+) 231 WP_054202591.1 DNA gyrase inhibitor YacG -
  ACLKMI_RS10385 (ACLKMI_10385) - 2179016..2179504 (-) 489 WP_413400825.1 Spy/CpxP family protein refolding chaperone -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44200.09 Da        Isoelectric Point: 9.8442

>NTDB_id=1090668 ACLKMI_RS10360 WP_413400821.1 2173439..2174668(+) (pilC) [Pseudoalteromonas sp. KJ71-7]
MAKKQEDRGLDTFQWVGVSARGKKLEGELSGSSIALVKAQLRKQGITPSKVKRKAKPLFGLQSVKKITPKDIALVTRQIA
TMLMAGVPLIQSIEMIGSGSTNKSVSKLMSAIGDEVKAGLPLSNALRKYPRYFDDLYCDLVASGEQSGSLDKIFDRVALY
KEKSEALKSKIKKAMFYPAAVLVVAFVVTSILLIFVVPQFQDIFSGFGAELPAFTLFVIGISEFMQANWWIALIGVVAFG
YIFKEAHLRSLKLRDATDRAILKLPVVGMILNKAAVARYARTLSTTFAAGVPLVDALDSAAGASGNAVYRYAILEIKAEV
SSGNQMNWAMRNSKIFPDMVIQMVAIGEESGSLDGMLAKVATIYEQEVDDAVDGLSSLLEPLIMAVLGVLVGGLIVAMYL
PIFQLGSVI

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=1090668 ACLKMI_RS10360 WP_413400821.1 2173439..2174668(+) (pilC) [Pseudoalteromonas sp. KJ71-7]
GTGGCAAAAAAGCAAGAAGACAGAGGCTTAGATACCTTCCAATGGGTGGGTGTTAGTGCTCGAGGGAAAAAGCTTGAAGG
TGAATTATCTGGCTCAAGCATAGCTTTAGTTAAGGCACAATTACGCAAACAAGGTATTACCCCCTCAAAAGTAAAACGTA
AAGCCAAGCCCTTGTTTGGCTTGCAAAGCGTGAAAAAAATCACCCCTAAAGATATTGCTTTAGTTACTCGACAAATTGCT
ACTATGTTAATGGCGGGTGTACCGCTGATACAATCCATTGAAATGATAGGCTCAGGCTCCACCAATAAAAGCGTGAGTAA
GCTCATGAGTGCTATTGGCGATGAAGTAAAAGCGGGGTTACCGCTTTCTAACGCACTTAGGAAATACCCTCGTTATTTTG
ATGATTTATATTGCGATTTAGTAGCCTCAGGCGAACAGTCGGGCTCGCTTGATAAAATATTTGACCGCGTTGCGCTTTAT
AAAGAAAAGTCAGAAGCACTTAAATCAAAAATTAAAAAAGCCATGTTTTATCCAGCAGCAGTGTTAGTTGTGGCGTTCGT
TGTTACCTCAATATTGCTGATATTTGTAGTACCGCAATTTCAAGATATCTTTTCTGGCTTTGGTGCCGAACTGCCAGCCT
TTACCTTATTTGTGATTGGCATTTCAGAATTTATGCAAGCAAATTGGTGGATAGCCCTCATTGGCGTTGTGGCATTTGGT
TATATATTTAAAGAAGCACATTTAAGAAGTTTAAAACTGCGTGATGCCACCGACCGTGCCATACTTAAACTCCCTGTTGT
CGGCATGATTTTAAATAAAGCTGCCGTGGCACGTTATGCCCGTACTTTATCGACTACTTTTGCTGCGGGTGTACCATTGG
TGGATGCTTTGGATTCTGCAGCTGGTGCCTCAGGCAACGCAGTTTACCGTTATGCTATTTTAGAAATAAAAGCCGAGGTA
AGCTCAGGCAACCAAATGAACTGGGCGATGCGTAACTCTAAAATATTCCCCGATATGGTAATACAAATGGTTGCCATTGG
TGAGGAGTCAGGATCGCTTGATGGCATGCTGGCTAAAGTTGCCACTATTTATGAGCAAGAAGTGGATGACGCAGTAGATG
GCTTATCTAGCTTACTTGAGCCATTAATCATGGCGGTGTTAGGCGTATTAGTTGGCGGCTTAATTGTGGCCATGTACTTA
CCTATATTCCAACTAGGTTCAGTTATTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(280-401)

Type II secretion system (T2SS), protein F (T2SSF)

(76-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

64.736

97.066

0.628
  pilC Acinetobacter baylyi ADP1

56.5

97.8

0.553
  pilC Acinetobacter baumannii D1279779

55.75

97.8

0.545
  pilC Legionella pneumophila strain ERS1305867

54.975

98.289

0.54
  pilC Vibrio cholerae strain A1552

44.527

98.289

0.438
  pilC Vibrio campbellii strain DS40M4

43.317

98.778

0.428
  pilG Neisseria meningitidis 44/76-A

43.478

95.599

0.416
  pilG Neisseria gonorrhoeae MS11

43.223

95.599

0.413

Multiple sequence alignment