Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACLKMI_RS10375 Genome accession   NZ_CP178625
Coordinates   2176984..2178660 (+) Length   558 a.a.
NCBI ID   WP_413400824.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. KJ71-7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2171984..2183660
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKMI_RS10355 (ACLKMI_10355) - 2172884..2173345 (+) 462 WP_413400820.1 pilin -
  ACLKMI_RS10360 (ACLKMI_10360) pilC 2173439..2174668 (+) 1230 WP_413400821.1 type II secretion system F family protein Machinery gene
  ACLKMI_RS10365 (ACLKMI_10365) pilD 2175305..2176216 (+) 912 WP_413400822.1 prepilin peptidase Machinery gene
  ACLKMI_RS10370 (ACLKMI_10370) coaE 2176219..2176848 (+) 630 WP_413400823.1 dephospho-CoA kinase -
  ACLKMI_RS10375 (ACLKMI_10375) pilB 2176984..2178660 (+) 1677 WP_413400824.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLKMI_RS10380 (ACLKMI_10380) yacG 2178726..2178956 (+) 231 WP_054202591.1 DNA gyrase inhibitor YacG -
  ACLKMI_RS10385 (ACLKMI_10385) - 2179016..2179504 (-) 489 WP_413400825.1 Spy/CpxP family protein refolding chaperone -
  ACLKMI_RS10390 (ACLKMI_10390) - 2179671..2180360 (+) 690 WP_413400826.1 response regulator -
  ACLKMI_RS10395 (ACLKMI_10395) - 2180338..2181765 (+) 1428 WP_413400827.1 ATP-binding protein -
  ACLKMI_RS10400 (ACLKMI_10400) - 2181755..2182699 (+) 945 WP_409423962.1 alpha/beta fold hydrolase -
  ACLKMI_RS10405 (ACLKMI_10405) - 2182696..2183508 (-) 813 WP_413402879.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62306.65 Da        Isoelectric Point: 5.9290

>NTDB_id=1090670 ACLKMI_RS10375 WP_413400824.1 2176984..2178660(+) (pilB) [Pseudoalteromonas sp. KJ71-7]
MNINSPLLRKFITLGRVDTDTVKARQQDFASTAELISKCGKIDSNDLAEQCIDLFRVPFFDLKDFNVAQIPAELVKEKLI
RKHHILPLVQKGRKVYIAASDPTDYGAFENFEFSTGLSCEVVVVDYLQLDHKIEQLLDAAGSLNLSEDEFKEFAELDSEP
QKDHTPKDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVASPPNTLASKLSARIKVMSRLDI
AEKRKPQDGRIKLKVTERKSIDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYLDALEQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGVNQVQINIKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCSKCKTPEQLPREELIRQGFSEQQIQDMVL
YAPKGCESCTEGYKGRVGIYEVMKITPEIAQIIMRGGNSLEIAEVSLKAGYNNLRLSGIRKAAEGVTSLAEINRVTSF

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=1090670 ACLKMI_RS10375 WP_413400824.1 2176984..2178660(+) (pilB) [Pseudoalteromonas sp. KJ71-7]
ATGAATATTAACTCACCGTTACTAAGAAAATTTATTACCCTCGGCCGTGTCGATACCGATACCGTAAAAGCCAGGCAGCA
AGACTTTGCGTCAACCGCAGAACTTATTTCTAAATGCGGAAAAATAGATAGCAACGATTTAGCTGAGCAATGTATTGATT
TGTTTAGAGTGCCTTTTTTTGATTTAAAAGACTTTAATGTTGCACAAATTCCAGCAGAGCTGGTTAAAGAAAAGCTAATT
CGCAAGCACCATATTTTACCTTTGGTACAAAAAGGCAGAAAAGTATATATTGCCGCCTCAGATCCTACCGATTATGGTGC
CTTTGAAAACTTTGAATTTAGCACCGGTTTATCGTGTGAAGTGGTGGTGGTTGACTACCTACAGCTTGACCATAAAATTG
AGCAACTATTGGATGCTGCAGGTAGCTTAAATTTAAGTGAGGATGAATTTAAAGAGTTTGCTGAACTAGATTCTGAGCCA
CAAAAAGACCATACGCCAAAAGACGATGACAAAGACGACGCCCCTATTATTGTTTACATTAATAAAATTTTAATGGACGC
AATAAAAAAAGGCGCCTCAGACTTACACTTTGAACCTTATGAACATAAATACCGTATTCGCTTTCGCATCGACGGAATTT
TACATGAGGTAGCCAGCCCGCCAAACACCTTGGCCAGTAAGCTATCTGCCCGTATAAAAGTTATGTCGCGCCTTGATATC
GCTGAAAAACGTAAGCCACAAGATGGCCGTATAAAACTCAAAGTGACAGAACGTAAAAGTATTGATTTTCGTGTTAGTAC
CATGCCTACCTTATGGGGCGAAAAAATTGTAATGCGTATCCTCGATTCATCAAGTGCCATGTTAGGTATTGATGTATTAG
GTTATGAACCTGAGCAGAAAAAACTCTACCTCGATGCATTAGAGCAACCACAGGGGATGATTTTAGTTACCGGCCCAACT
GGCTCAGGTAAAACCGTCTCCCTGTATACGGGTTTAAATATACTCAATCAGCCAGAGCGAAACATCAGTACCGCCGAAGA
TCCGGTAGAAATTAACCTCGAAGGGGTTAATCAGGTACAAATAAACATTAAAGCCGACATGACCTTTGCTAATGCTTTAC
GAGCGTTCTTACGTCAAGATCCCGATGTAGTAATGGTAGGTGAAATACGAGATTTAGAAACCGCTGAAATATCAATAAAA
GCGGCGCAAACCGGTCACTTAGTACTATCAACGCTACATACTAACTCTGCACCTGAAACCATAACTCGTTTGTTAAACAT
GGGCGTACCCGCGTATAATGTGGCCAGTTCAATTAGCTTAATAATCGCTCAGCGATTAGCGCGCCGATTATGCTCTAAAT
GTAAAACGCCAGAGCAATTACCGCGTGAAGAACTTATTCGCCAAGGCTTTAGTGAACAACAAATACAGGATATGGTTTTG
TATGCCCCTAAAGGCTGTGAAAGCTGTACTGAGGGCTATAAAGGCCGTGTTGGTATTTACGAGGTTATGAAAATCACCCC
CGAAATAGCGCAAATTATAATGCGTGGTGGTAACTCATTAGAAATTGCTGAGGTATCACTTAAAGCAGGGTATAACAATT
TACGTTTATCGGGTATTCGTAAAGCAGCAGAGGGTGTGACCTCGCTGGCTGAGATAAACCGCGTTACTAGTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)

Type II/IV secretion system protein (T2SSE)

(182-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.778

96.774

0.559
  pilB Acinetobacter baylyi ADP1

58.812

90.502

0.532
  pilB Legionella pneumophila strain ERS1305867

55.796

91.219

0.509
  pilB Vibrio parahaemolyticus RIMD 2210633

52.82

95.341

0.504
  pilB Vibrio cholerae strain A1552

52.523

95.878

0.504
  pilB Vibrio campbellii strain DS40M4

51.124

95.699

0.489
  pilF Neisseria gonorrhoeae MS11

49.425

93.548

0.462

Multiple sequence alignment