Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACJQW4_RS03670 Genome accession   NZ_CP178606
Coordinates   787516..789219 (+) Length   567 a.a.
NCBI ID   WP_241628719.1    Uniprot ID   -
Organism   Acinetobacter sp. A7.4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 782516..794219
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJQW4_RS03630 (ACJQW4_03630) nusA 783276..784760 (-) 1485 WP_241628339.1 transcription termination factor NusA -
  ACJQW4_RS03635 (ACJQW4_03635) rimP 784796..785320 (-) 525 WP_241628338.1 ribosome maturation factor RimP -
  ACJQW4_RS03660 (ACJQW4_03660) secG 786103..786432 (-) 330 WP_100242739.1 preprotein translocase subunit SecG -
  ACJQW4_RS03665 (ACJQW4_03665) tpiA 786446..787237 (-) 792 WP_241628717.1 triose-phosphate isomerase -
  ACJQW4_RS03670 (ACJQW4_03670) pilB 787516..789219 (+) 1704 WP_241628719.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACJQW4_RS03675 (ACJQW4_03675) pilC 789248..790474 (+) 1227 WP_241628721.1 type II secretion system F family protein Machinery gene
  ACJQW4_RS03680 (ACJQW4_03680) pilD 790474..791334 (+) 861 WP_241628723.1 prepilin peptidase Machinery gene
  ACJQW4_RS03685 (ACJQW4_03685) coaE 791337..791954 (+) 618 WP_241628725.1 dephospho-CoA kinase -
  ACJQW4_RS03690 (ACJQW4_03690) - 791951..792865 (-) 915 WP_413351718.1 DMT family transporter -
  ACJQW4_RS03695 (ACJQW4_03695) rlmB 792901..793653 (-) 753 WP_241628729.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACJQW4_RS03700 (ACJQW4_03700) - 793790..794113 (-) 324 WP_016168405.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 63417.86 Da        Isoelectric Point: 5.0271

>NTDB_id=1090388 ACJQW4_RS03670 WP_241628719.1 787516..789219(+) (pilB) [Acinetobacter sp. A7.4]
MTALQNSPRFTGFIRRLVEDGLVSTENMHIAVTNAKKSNQDIVPYLIENLKLKSLVIAEKISEEFGEPLFDLSTYDSSHI
LKDIVEDKLVTKFRILPIFKRGHLLFVATSNPTNIEAMDAIRFNSKLNIEVIIVEHDKLEKIIEQNFAEDTTFHFDDDFE
IDLQSSDSEKEEDDEPQGDEAPIVKYINKLLVDAIRMGASDLHFEPYEKTYRVRYRVDGVLRQIATPPLQLANRLASRLK
VMSQMDIAEKRVPQDGRIKLKISKTKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPEQKALFIEALEKPQGM
LLITGPTGSGKTVSLYTGLNILNQEDTNISTAEDPVEINLEGINQVNVNNKVGLTFAAALRSFLRQDPDIVMVGEIRDLE
TAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKAPIEIPQNSLLEMGFTEQ
DLLHPDFQIYQPVGCSECREGYKGRVGIYEVMKVTPEISKLIMEDGNAIQIAEASAKLGFNNLRLSGLKKVMQGVTSLQE
VNRVTSE

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1090388 ACJQW4_RS03670 WP_241628719.1 787516..789219(+) (pilB) [Acinetobacter sp. A7.4]
ATGACAGCACTACAAAATTCTCCACGATTTACAGGTTTTATTCGCCGCTTGGTCGAAGATGGATTGGTTTCTACTGAAAA
CATGCATATTGCTGTAACCAATGCCAAGAAATCCAACCAAGATATCGTTCCTTATCTAATTGAGAACTTAAAACTTAAGT
CGCTGGTGATTGCCGAAAAAATTTCTGAAGAATTTGGTGAGCCCTTATTTGATCTGTCCACTTATGATTCTTCACATATT
TTAAAAGATATTGTTGAAGATAAACTTGTTACTAAATTCAGAATTTTACCCATTTTTAAACGTGGGCATCTTTTATTTGT
GGCAACCAGTAACCCCACAAATATTGAAGCCATGGATGCCATCCGTTTTAACAGTAAACTGAATATTGAAGTTATTATTG
TTGAACATGACAAATTAGAAAAAATCATTGAACAAAATTTTGCTGAAGACACCACCTTCCATTTTGATGATGACTTTGAG
ATTGATCTACAATCTTCTGATAGTGAAAAAGAGGAAGATGATGAACCTCAAGGCGATGAAGCCCCAATCGTCAAATATAT
TAATAAATTACTGGTTGATGCGATTCGAATGGGTGCATCTGACTTACATTTTGAACCCTATGAAAAAACCTATCGTGTAC
GTTACCGTGTTGATGGGGTGTTGCGTCAAATTGCCACGCCCCCATTACAGTTAGCCAATCGTTTAGCTTCACGCTTAAAA
GTTATGTCACAAATGGATATTGCTGAAAAGCGTGTACCTCAAGATGGTCGTATCAAGCTGAAAATCTCGAAAACCAAAGC
CATCGACTTCCGTGTCAACTCACTCCCAACACTGTTTGGTGAAAAGATTGTACTGCGTATCTTAGATCCTTCGAGTGCAA
TGTTAGGGATCGATGCGCTAGGTTATGAACCCGAACAAAAAGCACTCTTTATTGAAGCCCTAGAAAAACCACAAGGGATG
CTACTCATTACAGGTCCTACGGGTTCGGGTAAAACCGTTTCCCTCTATACAGGTCTCAATATCCTTAACCAAGAAGATAC
CAATATTTCCACAGCAGAAGATCCCGTCGAAATTAACTTGGAAGGGATTAATCAGGTCAACGTCAATAATAAAGTCGGAC
TCACCTTTGCTGCAGCGCTCAGATCTTTCTTACGTCAAGATCCTGACATTGTCATGGTCGGTGAGATTCGAGACCTAGAA
ACTGCTGAAATTGCGATTAAAGCCGCACAAACAGGTCATATGGTGATGTCAACCCTGCACACCAACAGCGCTCCAGAAAC
ACTGACCCGCTTAAGAAATATGGGCGTCCCTTCCTTTAACATTGCAACCTCTGTAAACTTAGTGATTGCACAACGTCTGG
CTCGACGACTCTGTTCACAATGTAAAGCACCGATTGAAATTCCTCAAAATAGTTTGTTGGAAATGGGCTTTACTGAACAA
GACCTCCTGCACCCAGATTTTCAGATTTATCAGCCGGTCGGCTGTTCTGAATGTCGAGAAGGTTATAAAGGTCGAGTGGG
GATTTATGAAGTAATGAAAGTCACACCTGAAATTTCTAAGCTTATTATGGAAGATGGTAATGCAATTCAAATTGCAGAAG
CATCGGCAAAATTGGGGTTTAATAATCTACGTCTTTCAGGTTTAAAGAAGGTCATGCAAGGGGTAACTTCACTGCAAGAA
GTAAATCGCGTGACCAGCGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.474

100

0.799
  pilB Acinetobacter baylyi ADP1

76.842

100

0.772
  pilB Legionella pneumophila strain ERS1305867

52.533

94.004

0.494
  pilB Vibrio cholerae strain A1552

49.113

99.471

0.489
  pilF Neisseria gonorrhoeae MS11

49.195

98.589

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

48.294

98.236

0.474
  pilB Vibrio campbellii strain DS40M4

49.903

91.182

0.455
  pilB/pilB1 Synechocystis sp. PCC 6803

34.88

100

0.384
  pilF Thermus thermophilus HB27

36.964

98.765

0.365

Multiple sequence alignment