Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACLTDJ_RS04155 Genome accession   NZ_CP178394
Coordinates   863651..864868 (-) Length   405 a.a.
NCBI ID   WP_317757544.1    Uniprot ID   -
Organism   Pseudomonas bubulae strain S31     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 858651..869868
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLTDJ_RS04120 (ACLTDJ_04120) - 859670..859843 (-) 174 WP_088378952.1 DUF3094 family protein -
  ACLTDJ_RS04125 (ACLTDJ_04125) - 859898..860425 (-) 528 WP_095018261.1 MOSC domain-containing protein -
  ACLTDJ_RS04130 (ACLTDJ_04130) - 860422..861051 (-) 630 WP_095001620.1 DUF1780 domain-containing protein -
  ACLTDJ_RS04135 (ACLTDJ_04135) - 861161..861850 (+) 690 WP_095001619.1 energy-coupling factor ABC transporter permease -
  ACLTDJ_RS04140 (ACLTDJ_04140) yacG 861963..862166 (-) 204 WP_016779444.1 DNA gyrase inhibitor YacG -
  ACLTDJ_RS04145 (ACLTDJ_04145) coaE 862163..862786 (-) 624 WP_095020319.1 dephospho-CoA kinase -
  ACLTDJ_RS04150 (ACLTDJ_04150) - 862788..863654 (-) 867 WP_165446503.1 prepilin peptidase -
  ACLTDJ_RS04155 (ACLTDJ_04155) pilC 863651..864868 (-) 1218 WP_317757544.1 type II secretion system F family protein Machinery gene
  ACLTDJ_RS04160 (ACLTDJ_04160) - 865080..865487 (+) 408 WP_413052384.1 pilin -
  ACLTDJ_RS04170 (ACLTDJ_04170) - 865836..867326 (+) 1491 WP_413052385.1 AlkA N-terminal domain-containing protein -
  ACLTDJ_RS04175 (ACLTDJ_04175) - 867329..867820 (+) 492 WP_095024070.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  ACLTDJ_RS04180 (ACLTDJ_04180) nadC 867943..868791 (-) 849 WP_095001607.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44165.96 Da        Isoelectric Point: 10.1831

>NTDB_id=1089449 ACLTDJ_RS04155 WP_317757544.1 863651..864868(-) (pilC) [Pseudomonas bubulae strain S31]
MATQAVKYSLYRWEGTDRNGIKTSGQITAEHHTMIRVQLRRQGITVSKIRRHYSHTLNAGKSVTALDITLFTRQLATLIS
AGITMQQALKVIANGFESRAMRQQVTELQQDIGAGSSLSAALRKKPAHFDPLFCSLVEAGELAGALDVLLQRVATYREKT
ATLKKKIKKAMTYPSAVLVVALAVCGILLIEVVPQFQGVFRSFDAQLPVLTLWVIGLSDSLQAHGLWLLLFCGITLAGLH
HRYKHCATWRQRVDRWLLKLPVMGNLLHMSAMARFARTLSTTLAAGVPLLEALNSVASATGNSLYKNAVLRLKKQVATGS
QIHVAMAAAGVFPGMAIQMTAIGEESGTLDSMLDQVAAYYESEVDTLVDNLTTLLEPIIMVVLGVMVGGLVIAMYLPIFN
LGRVI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1089449 ACLTDJ_RS04155 WP_317757544.1 863651..864868(-) (pilC) [Pseudomonas bubulae strain S31]
ATGGCAACACAGGCCGTTAAATACAGCCTTTACCGGTGGGAAGGCACCGATCGAAATGGCATTAAAACCAGCGGGCAAAT
CACGGCGGAACACCACACAATGATTCGGGTCCAGCTACGCCGCCAAGGTATTACCGTTAGTAAAATCCGCCGCCATTACA
GTCACACACTAAATGCGGGCAAATCGGTCACGGCACTGGATATCACCCTGTTCACCCGCCAACTGGCTACCCTGATCAGC
GCGGGCATAACTATGCAGCAAGCTCTGAAGGTTATCGCCAACGGTTTCGAGAGCCGTGCCATGCGCCAGCAGGTCACCGA
ACTCCAGCAAGACATCGGCGCAGGCTCCAGCCTGTCGGCGGCGCTGCGCAAAAAACCGGCACATTTCGATCCTCTGTTTT
GCAGCCTCGTCGAAGCTGGCGAACTGGCCGGAGCTCTGGACGTGTTGCTGCAACGGGTGGCGACCTACAGGGAAAAGACC
GCCACTCTGAAAAAGAAAATCAAAAAAGCCATGACCTACCCCAGCGCCGTGCTCGTGGTCGCGCTGGCGGTGTGCGGCAT
TTTGTTGATCGAAGTGGTTCCGCAGTTCCAGGGCGTTTTCCGCTCATTCGACGCGCAACTGCCAGTGCTTACCTTGTGGG
TGATCGGCCTGTCTGACAGCCTCCAGGCGCATGGTCTATGGCTGCTGCTTTTTTGCGGCATCACCCTTGCTGGCCTTCAC
CATCGCTACAAGCACTGCGCAACCTGGCGCCAGCGCGTTGATCGCTGGCTGCTGAAACTGCCCGTCATGGGCAATCTGCT
GCACATGTCAGCCATGGCCCGTTTTGCCCGCACGCTGTCGACGACCCTGGCTGCCGGCGTGCCATTGCTCGAAGCGCTGA
ACTCCGTAGCCAGCGCCACTGGCAATAGCCTGTACAAAAACGCGGTGCTACGACTGAAGAAGCAGGTTGCAACGGGTAGC
CAGATCCATGTGGCCATGGCCGCTGCCGGAGTATTCCCGGGCATGGCAATCCAGATGACAGCTATTGGCGAAGAATCCGG
CACATTGGACAGCATGCTTGACCAGGTTGCCGCCTATTATGAAAGCGAGGTCGACACTCTGGTCGACAACCTGACAACCT
TGCTGGAGCCGATAATCATGGTGGTACTTGGAGTCATGGTTGGCGGGCTGGTCATCGCCATGTACTTGCCGATATTCAAC
CTTGGCCGGGTAATATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

51.843

100

0.521
  pilC Acinetobacter baumannii D1279779

49.874

98.025

0.489
  pilC Acinetobacter baylyi ADP1

48.995

98.272

0.481
  pilC Legionella pneumophila strain ERS1305867

47.236

98.272

0.464
  pilC Vibrio cholerae strain A1552

41.728

100

0.417
  pilC Vibrio campbellii strain DS40M4

39.803

100

0.4
  pilG Neisseria gonorrhoeae MS11

38.308

99.259

0.38
  pilC Thermus thermophilus HB27

38

98.765

0.375
  pilG Neisseria meningitidis 44/76-A

38.482

94.321

0.363

Multiple sequence alignment