Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACLFK2_RS23670 Genome accession   NZ_CP177307
Coordinates   5125897..5127114 (+) Length   405 a.a.
NCBI ID   WP_003161763.1    Uniprot ID   A0A3P3S6C0
Organism   Pseudomonas aeruginosa strain Pa021     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5120897..5132114
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLFK2_RS23645 (ACLFK2_23645) nadC 5120995..5121843 (+) 849 WP_003161760.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACLFK2_RS23655 (ACLFK2_23655) - 5122024..5123421 (-) 1398 WP_225319908.1 O-antigen ligase family protein -
  ACLFK2_RS23660 (ACLFK2_23660) - 5123495..5123962 (-) 468 WP_003161762.1 pilin -
  ACLFK2_RS23665 (ACLFK2_23665) pilB 5124193..5125893 (+) 1701 WP_003122078.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLFK2_RS23670 (ACLFK2_23670) pilC 5125897..5127114 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  ACLFK2_RS23675 (ACLFK2_23675) pilD 5127115..5127987 (+) 873 WP_003107301.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ACLFK2_RS23680 (ACLFK2_23680) coaE 5127984..5128595 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  ACLFK2_RS23685 (ACLFK2_23685) yacG 5128592..5128792 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  ACLFK2_RS23690 (ACLFK2_23690) - 5128829..5129038 (-) 210 WP_003094660.1 hypothetical protein -
  ACLFK2_RS23695 (ACLFK2_23695) - 5129144..5129833 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  ACLFK2_RS23700 (ACLFK2_23700) - 5129830..5130300 (-) 471 WP_003103866.1 hypothetical protein -
  ACLFK2_RS23705 (ACLFK2_23705) - 5130297..5130722 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  ACLFK2_RS23710 (ACLFK2_23710) - 5130855..5131484 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  ACLFK2_RS23715 (ACLFK2_23715) - 5131481..5131930 (+) 450 WP_003161439.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43998.80 Da        Isoelectric Point: 9.8103

>NTDB_id=1086640 ACLFK2_RS23670 WP_003161763.1 5125897..5127114(+) (pilC) [Pseudomonas aeruginosa strain Pa021]
MADKALKTSVFVWEGTDKKGTKVKGELSSQNPTLVKAQLRKQGITPVKVRKKGISLLGAGKKIKPMDIALFTRQMSTMMA
AGVPLLQSFDIISEGFDNPNMRKLVEEIKQEVAGGNSLANSLRKKPQYFDSLYCNLVDAGEQSGALETLLDRVATYKEKT
EALKAKIKKAMTYPIAVIVVAIIVSAILLIKVVPQFQSVFEGFGAELPAFTQMVINISNVLQEWWLLVLLMMGGAGFLLN
HAYKRSEKFRDATDRTVLKLPIVGAILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRDAVGKIKQDVSTGM
QLNFSMRTTNIFPSMAIQMTAIGEESGALDDMLAKVAGFYEQEVDNAVDNLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1086640 ACLFK2_RS23670 WP_003161763.1 5125897..5127114(+) (pilC) [Pseudomonas aeruginosa strain Pa021]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCGTATGGGAAGGCACTGACAAGAAAGGCACCAAGGTCAAGGGCGAGCT
GTCCAGCCAGAACCCGACCCTGGTCAAGGCGCAACTGCGCAAGCAGGGCATCACGCCGGTCAAGGTCCGCAAGAAAGGCA
TCTCCCTGCTGGGGGCGGGGAAGAAGATCAAGCCTATGGACATCGCCCTGTTCACCCGCCAGATGTCCACCATGATGGCC
GCCGGCGTGCCGCTGCTGCAGTCGTTCGACATCATCAGCGAAGGCTTCGACAATCCGAACATGCGCAAGCTGGTGGAGGA
GATCAAGCAGGAAGTTGCCGGCGGCAACAGCCTGGCCAACTCGCTGCGCAAGAAGCCGCAGTACTTCGACTCGCTCTACT
GCAACCTGGTGGACGCCGGCGAACAATCCGGCGCCCTGGAAACCCTGCTCGACCGGGTCGCCACCTACAAGGAAAAGACC
GAGGCGCTGAAGGCCAAGATCAAGAAGGCCATGACCTACCCGATCGCGGTGATCGTCGTCGCCATCATCGTTTCGGCGAT
CCTACTGATCAAGGTGGTGCCGCAGTTCCAGTCGGTGTTCGAGGGCTTCGGCGCCGAACTGCCGGCCTTCACCCAAATGG
TCATCAATATCTCCAACGTGCTCCAGGAATGGTGGCTCCTGGTACTGCTGATGATGGGCGGCGCCGGCTTCCTGCTGAAC
CACGCCTACAAGCGTTCGGAGAAGTTCCGCGACGCCACCGACCGCACGGTACTGAAGCTGCCTATCGTCGGCGCCATCCT
CTACAAATCCGCCGTGGCACGCTACGCGCGGACGCTGTCGACCACCTTCGCCGCCGGCGTGCCATTGGTGGAAGCCCTCG
ACTCGGTTTCCGGCGCCACCGGCAACGTGGTGTTCAGGGACGCGGTGGGCAAGATCAAGCAGGACGTGTCCACCGGCATG
CAGCTGAACTTCTCCATGCGCACCACCAACATCTTCCCCAGCATGGCCATCCAGATGACCGCCATCGGCGAGGAGTCGGG
CGCGCTGGACGACATGCTGGCCAAGGTCGCTGGCTTCTACGAGCAGGAAGTGGATAATGCCGTGGACAACCTGACCGCCC
TGATGGAACCGATGATCATGGCGGTCCTCGGGGTCCTGGTCGGTGGCCTGATCATCGCCATGTACCTGCCGATCTTCCAG
CTGGGCAACGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3P3S6C0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

78.765

100

0.788
  pilC Acinetobacter baylyi ADP1

61.275

100

0.617
  pilC Acinetobacter baumannii D1279779

60.644

99.753

0.605
  pilC Legionella pneumophila strain ERS1305867

54.293

97.778

0.531
  pilG Neisseria gonorrhoeae MS11

46.287

99.753

0.462
  pilG Neisseria meningitidis 44/76-A

45.771

99.259

0.454
  pilC Vibrio cholerae strain A1552

41.728

100

0.417
  pilC Vibrio campbellii strain DS40M4

40.898

99.012

0.405
  pilC Thermus thermophilus HB27

38.155

99.012

0.378

Multiple sequence alignment