Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACLINX_RS15220 Genome accession   NZ_CP176876
Coordinates   3357563..3358888 (+) Length   441 a.a.
NCBI ID   WP_411566709.1    Uniprot ID   -
Organism   Pseudomonas orientalis strain MPB27     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3352563..3363888
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLINX_RS15200 (ACLINX_15200) - 3352640..3355102 (-) 2463 WP_411566708.1 penicillin-binding protein 1A -
  ACLINX_RS15205 (ACLINX_15205) pilM 3355321..3355977 (+) 657 WP_243353200.1 type IV pilus biogenesis protein PilM -
  ACLINX_RS15210 (ACLINX_15210) - 3355974..3356525 (+) 552 WP_181282854.1 PilN domain-containing protein -
  ACLINX_RS15215 (ACLINX_15215) - 3356522..3357493 (+) 972 WP_411566086.1 pilus assembly protein PilP -
  ACLINX_RS15220 (ACLINX_15220) pilQ 3357563..3358888 (+) 1326 WP_411566709.1 type IV pilus secretin PilQ Machinery gene
  ACLINX_RS15225 (ACLINX_15225) aroK 3358893..3359411 (+) 519 WP_124356688.1 shikimate kinase AroK -
  ACLINX_RS15230 (ACLINX_15230) aroB 3359537..3360637 (+) 1101 WP_191952714.1 3-dehydroquinate synthase -
  ACLINX_RS15235 (ACLINX_15235) - 3360648..3362222 (+) 1575 WP_411566087.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 47819.80 Da        Isoelectric Point: 5.2501

>NTDB_id=1083502 ACLINX_RS15220 WP_411566709.1 3357563..3358888(+) (pilQ) [Pseudomonas orientalis strain MPB27]
MAAAVPNRVDLIHLPPPESKPLASYTGDKLNLNFQNIELRAALQQIADVAGLNLVAGDEVQGSITLRLKDVPWDQALDLV
LQAKGLDKRVKAGVLLVAPADELAARELLTLEARKQMAELAPLRRELLQVNYAKAADLAKLFQSVTGFEGATDERGSVAV
DDRTNNIIAYQTAERLEELRRIVAQLDVPVRQVMIEARIVEANVDYDKSLGVRWGGRLNRGNWGAGGISKPLADGAQPAQ
GPPSSPFVDLGSLTGTSGLGLAFITDNLLLDLELTAMEKSGNGEIVSQPKVVTSDKETARILKGTEIPYQESTSQGATSV
SFKEASLSLEVTPQITPDDHVIMVVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVKNGETIVIGGVFSNTQSKVVDKVPF
LGDVPYLGRLFRRDVRAEKKSELLVFLTPRIMNNQAIAASR

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=1083502 ACLINX_RS15220 WP_411566709.1 3357563..3358888(+) (pilQ) [Pseudomonas orientalis strain MPB27]
ATGGCTGCCGCTGTACCCAATCGCGTCGACCTTATCCATCTGCCGCCGCCCGAAAGTAAGCCATTGGCCAGCTATACGGG
CGACAAACTGAACCTTAATTTCCAGAACATCGAATTGCGCGCGGCATTGCAGCAAATCGCCGACGTCGCGGGCCTGAACC
TGGTGGCCGGTGACGAGGTACAGGGTTCGATCACCCTGCGGCTCAAGGACGTGCCCTGGGACCAGGCACTGGACCTGGTG
TTGCAAGCCAAGGGGCTGGACAAGCGGGTGAAGGCCGGCGTCTTGCTGGTGGCCCCCGCCGATGAATTGGCCGCACGCGA
ACTGCTGACGCTGGAGGCGCGCAAACAGATGGCTGAGCTGGCGCCGTTACGCCGTGAGTTGCTGCAGGTCAATTACGCCA
AGGCGGCGGACCTGGCCAAGCTGTTCCAGTCCGTGACCGGCTTTGAAGGCGCGACCGACGAGCGCGGCTCGGTGGCGGTG
GATGACCGTACCAACAATATCATCGCCTACCAGACCGCAGAGCGCCTGGAAGAACTGCGGCGGATCGTGGCGCAACTGGA
CGTCCCGGTACGCCAGGTGATGATCGAGGCGCGCATTGTAGAGGCCAATGTCGATTACGACAAAAGCCTGGGGGTGCGTT
GGGGCGGCCGGCTAAATCGCGGCAATTGGGGCGCCGGGGGAATTTCCAAACCATTGGCGGACGGTGCTCAGCCCGCACAG
GGCCCGCCCAGCTCACCCTTTGTCGACCTGGGCTCGCTGACCGGCACCTCCGGCCTGGGTCTGGCCTTTATCACCGACAA
CCTGTTGCTGGACCTTGAGCTGACCGCCATGGAAAAGTCCGGCAACGGCGAAATCGTCTCACAGCCCAAGGTGGTCACCT
CGGACAAGGAAACCGCGCGCATCCTCAAGGGCACCGAGATTCCCTATCAGGAATCCACGTCCCAGGGCGCCACCTCGGTG
TCGTTCAAGGAAGCCTCGCTGTCATTGGAGGTTACACCGCAGATCACCCCCGACGATCATGTGATCATGGTGGTCAAAGT
CACCAAGGACGAGCCCGACTACCTGAACAAACTCAACGACGTTCCGCCGATCAAGAAAAACGAGGTCAACGCCAAGGTCC
TGGTCAAGAATGGCGAGACCATTGTCATCGGCGGGGTTTTCTCCAATACCCAAAGCAAAGTGGTAGATAAAGTGCCATTT
TTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGCGATGTGCGGGCGGAGAAAAAATCCGAGCTGCTGGTATTCCT
GACTCCGCGTATCATGAATAACCAGGCGATTGCTGCGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(53-99)

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)

Bacterial type II and III secretion system protein (Secretin)

(276-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

70.481

99.093

0.698
  pilQ Acinetobacter baumannii D1279779

40.891

100

0.458
  pilQ Acinetobacter baumannii strain A118

40.891

100

0.458
  comQ Acinetobacter baylyi ADP1

43.077

100

0.444
  pilQ Legionella pneumophila strain Lp02

44.286

95.238

0.422
  pilQ Vibrio campbellii strain DS40M4

43.155

97.732

0.422
  pilQ Legionella pneumophila strain ERS1305867

43.81

95.238

0.417
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.89

97.279

0.417
  pilQ Vibrio cholerae strain A1552

42.89

97.279

0.417

Multiple sequence alignment