Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACK1O1_RS15655 Genome accession   NZ_CP176505
Coordinates   3435046..3436773 (-) Length   575 a.a.
NCBI ID   WP_409460401.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain PSKL1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3430046..3441773
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK1O1_RS15640 (ACK1O1_15640) sucC 3430364..3431533 (-) 1170 WP_004154430.1 ADP-forming succinate--CoA ligase subunit beta -
  ACK1O1_RS15645 (ACK1O1_15645) - 3431916..3433529 (+) 1614 WP_249843092.1 sensor histidine kinase -
  ACK1O1_RS15650 (ACK1O1_15650) pilR 3433590..3434963 (+) 1374 WP_249843091.1 sigma-54-dependent transcriptional regulator Regulator
  ACK1O1_RS15655 (ACK1O1_15655) pilB 3435046..3436773 (-) 1728 WP_409460401.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACK1O1_RS15660 (ACK1O1_15660) - 3436900..3437805 (-) 906 WP_409460402.1 sterol desaturase family protein -
  ACK1O1_RS15665 (ACK1O1_15665) - 3437908..3438348 (-) 441 WP_057498795.1 pilin -
  ACK1O1_RS15670 (ACK1O1_15670) pilC 3438710..3439969 (+) 1260 WP_309334227.1 type II secretion system F family protein Machinery gene
  ACK1O1_RS15675 (ACK1O1_15675) - 3439978..3440841 (+) 864 WP_107231736.1 prepilin peptidase -
  ACK1O1_RS15680 (ACK1O1_15680) coaE 3440853..3441464 (+) 612 WP_409460403.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62299.26 Da        Isoelectric Point: 5.8733

>NTDB_id=1080505 ACK1O1_RS15655 WP_409460401.1 3435046..3436773(-) (pilB) [Stenotrophomonas maltophilia strain PSKL1]
MNAASPPNLVGITGLARRLVQDGALDEATARDAMAKATAARQPLPTWFAQNKLVSASQLAAANAVEFGMPLFDVSTFDAS
QNAMNLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHALDEIKFHTNLVVETILVDEDQIRRTLEQWHASHDTLGDTLGGD
DEGMANLDVGLGDEDNAGGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVKL
NQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLFM
DAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDII
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCPNCKRPAQLPEHA
LLAEGFSQAQLDAGIELYEAVGCDECTEGYKGRTGVYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKKAA
AGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1080505 ACK1O1_RS15655 WP_409460401.1 3435046..3436773(-) (pilB) [Stenotrophomonas maltophilia strain PSKL1]
ATGAACGCTGCCTCCCCCCCCAATCTGGTCGGCATCACCGGCCTGGCCCGGCGCCTGGTCCAGGACGGTGCCCTGGACGA
GGCCACCGCGCGCGATGCCATGGCCAAGGCCACGGCGGCCCGCCAGCCACTGCCCACCTGGTTCGCGCAGAACAAGCTGG
TCAGCGCCTCGCAGCTGGCGGCGGCCAATGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACGTTCGATGCCAGC
CAGAACGCGATGAACCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAGCGCGGCGGCAAGCT
GTTCGTGGGCACCAGCAATCCGACCCACGCGCTGGATGAGATCAAGTTCCACACCAATCTGGTGGTGGAGACCATCCTGG
TCGACGAGGATCAGATCCGCCGCACCCTGGAGCAGTGGCACGCCAGCCATGACACGCTGGGCGACACCCTCGGCGGCGAC
GACGAGGGGATGGCCAACCTCGATGTTGGTCTGGGCGACGAAGACAATGCCGGTGGCGACTCCGGCATCGACGCCAAGGG
CGATGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGATGCCATCCGCAAGGGCGCCTCGGACATCCACTTCG
AGCCGTACGAAGACGACTATCGCGTGCGCCTGCGCATCGATGGCCTGTTGAAGATGGTCGCGCGTGCGCCGGTGAAGCTG
AACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGATATCGCCGAAAAGCGCGTGCCGCAGGACGGGCGCAT
CAAGCTGAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGTGAGAAGGTGGTGC
TGCGTATCCTGGATGGCAGCGCCGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAGCAGAAGCTGTTCATG
GATGCCATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGCTCGGGCAAGACGGTGTCGCTGTATACCGC
GCTGGGCATCCTCAACGACGAGACCCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGCGTCAACC
AGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCCGCACTGCGCTCGTTCCTGCGCCAGGATCCGGACATCATC
ATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCCATCAAGGCGGCGCAGACCGGTCACATGGTGCTGTCCAC
GCTGCATACCAACGATGCGCCGCAGACCATCGCGCGACTGATGAACATGGGCATTGCGCCGTACAACATCACCAGTTCGG
TGACGCTGGTGATCGCCCAGCGCTTGGCGCGCCGTCTGTGCCCGAACTGCAAGCGCCCGGCGCAGTTGCCCGAACACGCG
CTGTTGGCCGAAGGCTTCAGCCAGGCACAGCTGGATGCGGGCATCGAGCTGTATGAGGCGGTGGGCTGCGATGAATGCAC
CGAAGGCTACAAGGGGCGTACCGGCGTCTACCAGGTGATGCCGATGACCGATGAGATCGCAACCATTGTGCTGGCCGGCG
GTAATGCACTGCAGATTGCCGAGGCGGCGCAGCAGATTGGAGTGAATGATCTGCGCCAGTCGGCGTTGAAGAAGGCCGCG
GCCGGCGTCACCAGCCTGGCCGAGATCAACCGCGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)

Type II/IV secretion system protein (T2SSE)

(198-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.614

98.609

0.558
  pilB Acinetobacter baylyi ADP1

55.749

99.826

0.557
  pilB Legionella pneumophila strain ERS1305867

51.489

99.304

0.511
  pilF Neisseria gonorrhoeae MS11

48.768

98.783

0.482
  pilB Vibrio cholerae strain A1552

48.763

98.435

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

46.195

98.261

0.454
  pilB Vibrio campbellii strain DS40M4

44.991

98.957

0.445
  pilB/pilB1 Synechocystis sp. PCC 6803

36.007

100

0.383
  pilF Thermus thermophilus HB27

37.746

97.217

0.367
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.095

94.957

0.362

Multiple sequence alignment