Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACJ7VY_RS04310 Genome accession   NZ_CP176501
Coordinates   965821..966951 (+) Length   376 a.a.
NCBI ID   WP_104938227.1    Uniprot ID   -
Organism   Stenotrophomonas sp. Y-13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 960821..971951
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ7VY_RS04295 (ACJ7VY_04295) - 961490..963445 (+) 1956 WP_104938224.1 esterase/lipase family protein -
  ACJ7VY_RS04300 (ACJ7VY_04300) proC 963541..964380 (-) 840 WP_409476430.1 pyrroline-5-carboxylate reductase -
  ACJ7VY_RS04305 (ACJ7VY_04305) pilT 964648..965685 (+) 1038 WP_104938226.1 type IV pilus twitching motility protein PilT Machinery gene
  ACJ7VY_RS04310 (ACJ7VY_04310) pilU 965821..966951 (+) 1131 WP_104938227.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACJ7VY_RS04315 (ACJ7VY_04315) - 967462..968100 (+) 639 WP_240211687.1 DUF3617 domain-containing protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41821.12 Da        Isoelectric Point: 7.0221

>NTDB_id=1080471 ACJ7VY_RS04310 WP_104938227.1 965821..966951(+) (pilU) [Stenotrophomonas sp. Y-13]
MSTIDFTSFLKLMAHQKASDLFITAGMPPSMKVHGRISPITQTPLTPQQSRDMVLNVMTPAQREEFEKTHECNFAIGLSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELNLPPVIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWDAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLATLHANNANQAM
DRIINFFPEDRRSQLLMDLSLNLKGVVAQQLVPTPDGKGRRVAMEILLGTPLVQDYIRDGEIHKLKEVMKESVQLGMKTF
DQSLFELYQAGEISYDDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEIAGTR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1080471 ACJ7VY_RS04310 WP_104938227.1 965821..966951(+) (pilU) [Stenotrophomonas sp. Y-13]
ATGAGCACCATTGATTTCACTTCATTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACCGCCGGCAT
GCCGCCGTCGATGAAGGTGCACGGCCGGATCAGCCCGATCACGCAGACGCCGCTGACCCCGCAGCAGAGCCGCGACATGG
TGCTCAACGTGATGACGCCGGCGCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCCTGTCCGGC
GTGGGCCGCTTCCGCGTCAGCTGTTTCTACCAGCGCAACCAGGTGGGCATGGTGCTGCGCCGGATCGAGACGCGGATCCC
GACGGTGGAAGAGCTGAACCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCCTGTTCGTCGGCG
CCACCGGTACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCGACCGGGCACATCATCACC
ATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGATACCGACAG
CTGGGACGCGGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTCCGCACCCGCGAGGGCA
TGGACCATGCCATCGCCTTCGCCGAGACCGGCCACCTGGTGCTGGCGACGCTGCATGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAGGACCGCCGCAGCCAGCTGCTGATGGACCTGTCGCTCAACCTCAAGGGCGTGGT
CGCGCAGCAGCTGGTGCCGACGCCCGATGGCAAGGGGCGCCGGGTGGCGATGGAGATCCTGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAAGTGATGAAGGAATCGGTGCAGCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGACGACGCGCTGCGCTACGCCGACTCGCAGAACGA
AGTACGCCTGCGCATCAAGCTCTCGCAGGGCGGCGATGCCAAGACCCTGTCGCAGGGGCTCGATGGCGTGGAGATCGCGG
GTACGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.665

97.606

0.67
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

54.213

94.681

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.417

94.947

0.412
  pilT Acinetobacter nosocomialis M2

39.535

91.489

0.362
  pilT Acinetobacter baumannii D1279779

39.535

91.489

0.362
  pilT Acinetobacter baumannii strain A118

39.535

91.489

0.362

Multiple sequence alignment