Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACJ3W8_RS13525 Genome accession   NZ_CP176466
Coordinates   2833850..2835079 (+) Length   409 a.a.
NCBI ID   WP_409423955.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. RW-H-Ap-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2828850..2840079
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ3W8_RS13505 (ACJ3W8_13505) ampE 2829820..2830670 (-) 851 Protein_2597 beta-lactamase regulator AmpE -
  ACJ3W8_RS13510 (ACJ3W8_13510) ampD 2830958..2831503 (-) 546 WP_409423952.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACJ3W8_RS13515 (ACJ3W8_13515) nadC 2831903..2832748 (+) 846 WP_409423953.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACJ3W8_RS13520 (ACJ3W8_13520) - 2833298..2833756 (+) 459 WP_409423954.1 pilin -
  ACJ3W8_RS13525 (ACJ3W8_13525) pilC 2833850..2835079 (+) 1230 WP_409423955.1 type II secretion system F family protein Machinery gene
  ACJ3W8_RS13530 (ACJ3W8_13530) - 2835714..2836625 (+) 912 WP_409423956.1 prepilin peptidase -
  ACJ3W8_RS13535 (ACJ3W8_13535) coaE 2836628..2837257 (+) 630 WP_409423957.1 dephospho-CoA kinase -
  ACJ3W8_RS13540 (ACJ3W8_13540) pilB 2837393..2839069 (+) 1677 WP_409423958.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACJ3W8_RS13545 (ACJ3W8_13545) yacG 2839135..2839365 (+) 231 WP_058549398.1 DNA gyrase inhibitor YacG -
  ACJ3W8_RS13550 (ACJ3W8_13550) - 2839425..2839913 (-) 489 WP_409423959.1 Spy/CpxP family protein refolding chaperone -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44320.21 Da        Isoelectric Point: 9.7665

>NTDB_id=1080171 ACJ3W8_RS13525 WP_409423955.1 2833850..2835079(+) (pilC) [Pseudoalteromonas sp. RW-H-Ap-1]
MAKKQEDRGLDTFQWVGVSARGKKLEGELSGSSIALVKAQLRKQGITPSKVKRKAKPLFGLQSVKKITPKDIALVTRQIA
TMLMAGVPLIQSIEMIGSGSTNKSVSKLMSAIGDEVKAGLPLSNALRKHPRYFDDLYCDLVASGEQSGALDRIFDRVAIY
KEKSEALKSKIKKAMFYPAAVLVVAFVVTSILLIFVVPQFQDIFNGFGAELPAFTLFVIGISEFMQEYWWIALIGVVAFG
YIFKEAHLRSLKLRDATDRAILKLPVVGMILNKAAVARYARTLSTTFAAGVPLVDALDSAAGASGNAVYRYAILEIKAEV
SSGNQMNWAMRNSKIFPDMVIQMVAIGEESGSLDGMLAKVATIYEQEVDDAVDGLSSLLEPLIMAVLGVLVGGLIVAMYL
PIFQLGSVI

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=1080171 ACJ3W8_RS13525 WP_409423955.1 2833850..2835079(+) (pilC) [Pseudoalteromonas sp. RW-H-Ap-1]
GTGGCAAAAAAGCAAGAAGACAGAGGCTTAGATACCTTCCAATGGGTGGGTGTTAGTGCTCGAGGGAAAAAGCTTGAAGG
TGAATTATCTGGCTCAAGCATAGCTTTAGTTAAGGCACAATTACGCAAACAAGGTATTACCCCCTCAAAAGTAAAACGTA
AAGCCAAGCCCTTGTTTGGCTTGCAAAGCGTGAAAAAAATCACCCCTAAAGATATTGCTTTAGTTACTCGACAAATTGCT
ACTATGTTAATGGCGGGTGTACCGCTGATACAATCCATTGAAATGATAGGCTCAGGCTCCACCAATAAAAGCGTGAGTAA
GCTCATGAGTGCTATTGGCGATGAAGTAAAAGCGGGGTTACCGCTTTCTAACGCACTTAGAAAACACCCCCGTTATTTTG
ATGATTTATATTGTGATTTAGTTGCATCAGGCGAGCAATCGGGTGCGCTTGATAGAATATTTGATCGTGTTGCTATTTAT
AAAGAAAAGTCTGAGGCATTAAAGTCAAAAATTAAAAAAGCCATGTTTTATCCAGCAGCAGTGTTAGTTGTGGCGTTCGT
TGTTACCTCAATATTGCTGATATTTGTAGTTCCTCAATTTCAAGACATTTTTAATGGCTTCGGCGCTGAACTGCCTGCAT
TTACTTTGTTTGTAATTGGTATATCTGAATTTATGCAAGAATATTGGTGGATAGCCCTCATTGGCGTTGTGGCATTTGGT
TATATATTTAAAGAAGCACATTTAAGAAGTTTAAAACTGCGTGATGCCACCGACCGTGCCATACTTAAACTCCCTGTTGT
CGGCATGATTTTAAATAAAGCTGCCGTGGCACGTTATGCCCGTACTTTATCGACTACTTTTGCTGCGGGTGTACCATTGG
TGGATGCTTTGGATTCTGCAGCTGGTGCCTCAGGCAACGCAGTTTACCGTTATGCTATTTTAGAAATAAAAGCCGAGGTT
AGCTCTGGTAACCAAATGAACTGGGCAATGCGTAACTCAAAAATATTCCCTGATATGGTAATACAAATGGTCGCGATTGG
TGAGGAGTCTGGTTCTTTGGATGGAATGCTTGCGAAAGTAGCCACTATTTACGAGCAAGAAGTTGATGATGCAGTTGATG
GCCTTTCAAGCTTACTTGAGCCATTAATTATGGCGGTGTTAGGCGTATTAGTTGGCGGCTTAATTGTGGCCATGTACTTA
CCTATATTCCAACTAGGTTCAGTTATTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(280-401)

Type II secretion system (T2SS), protein F (T2SSF)

(76-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

64.232

97.066

0.623
  pilC Acinetobacter baumannii D1279779

56.5

97.8

0.553
  pilC Acinetobacter baylyi ADP1

56.25

97.8

0.55
  pilC Legionella pneumophila strain ERS1305867

54.975

98.289

0.54
  pilC Vibrio cholerae strain A1552

44.527

98.289

0.438
  pilG Neisseria meningitidis 44/76-A

44.246

95.599

0.423
  pilC Vibrio campbellii strain DS40M4

42.928

98.533

0.423
  pilG Neisseria gonorrhoeae MS11

43.99

95.599

0.421

Multiple sequence alignment