Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACJ3W8_RS13540 Genome accession   NZ_CP176466
Coordinates   2837393..2839069 (+) Length   558 a.a.
NCBI ID   WP_409423958.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. RW-H-Ap-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2832393..2844069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ3W8_RS13520 (ACJ3W8_13520) - 2833298..2833756 (+) 459 WP_409423954.1 pilin -
  ACJ3W8_RS13525 (ACJ3W8_13525) pilC 2833850..2835079 (+) 1230 WP_409423955.1 type II secretion system F family protein Machinery gene
  ACJ3W8_RS13530 (ACJ3W8_13530) - 2835714..2836625 (+) 912 WP_409423956.1 prepilin peptidase -
  ACJ3W8_RS13535 (ACJ3W8_13535) coaE 2836628..2837257 (+) 630 WP_409423957.1 dephospho-CoA kinase -
  ACJ3W8_RS13540 (ACJ3W8_13540) pilB 2837393..2839069 (+) 1677 WP_409423958.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACJ3W8_RS13545 (ACJ3W8_13545) yacG 2839135..2839365 (+) 231 WP_058549398.1 DNA gyrase inhibitor YacG -
  ACJ3W8_RS13550 (ACJ3W8_13550) - 2839425..2839913 (-) 489 WP_409423959.1 Spy/CpxP family protein refolding chaperone -
  ACJ3W8_RS13555 (ACJ3W8_13555) - 2840080..2840769 (+) 690 WP_409423960.1 response regulator -
  ACJ3W8_RS13560 (ACJ3W8_13560) - 2840747..2842174 (+) 1428 WP_409423961.1 ATP-binding protein -
  ACJ3W8_RS13565 (ACJ3W8_13565) - 2842164..2843108 (+) 945 WP_409423962.1 alpha/beta fold hydrolase -
  ACJ3W8_RS13570 (ACJ3W8_13570) - 2843105..2843935 (-) 831 WP_409425593.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62325.60 Da        Isoelectric Point: 5.3434

>NTDB_id=1080172 ACJ3W8_RS13540 WP_409423958.1 2837393..2839069(+) (pilB) [Pseudoalteromonas sp. RW-H-Ap-1]
MNINSPLLRKFITLGRIDTDTVKARQQDFASTAELISKCGKIDSLDLAEQCIDLFRVPYFDLKDFNVAQIPAELVKEKLI
RKHHILPLVQKDRKVYIAASDPTDYGAFENFEFSTGLSCEVVVVDYIQLDNKIEQLLDATGSLNLSDDEFKEFAELDSEP
QKDHTPKDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVASPPNTLSTKLSARIKVMSRLDI
AEKRKPQDGRIKLKITERKSIDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYLDALEQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGVNQVQINPKAEMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTPEQLPSEELIRQGFSEQQIQDMVL
YAPKGCDSCTDGYKGRVGIYEVMQITPEIAQIIMRGGNSLEIAEVSLKAGFNNLRLSGIRKAAEGVTSLAEINRVTSF

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=1080172 ACJ3W8_RS13540 WP_409423958.1 2837393..2839069(+) (pilB) [Pseudoalteromonas sp. RW-H-Ap-1]
ATGAATATTAACTCACCGTTATTGAGAAAATTTATTACCCTTGGCCGTATCGATACCGATACCGTAAAAGCCAGGCAGCA
AGACTTTGCGTCAACTGCTGAGCTTATTTCTAAATGCGGAAAAATCGATAGCCTAGATTTAGCTGAGCAATGTATAGATT
TGTTTCGAGTACCCTATTTTGATTTAAAAGACTTTAATGTTGCACAAATTCCAGCAGAGCTGGTTAAAGAAAAGCTGATC
CGTAAGCACCATATTTTACCGCTTGTTCAAAAAGATAGAAAAGTATACATTGCCGCCTCAGATCCTACAGATTACGGTGC
CTTTGAAAACTTTGAATTTAGCACGGGTTTATCGTGTGAAGTGGTGGTGGTTGATTACATTCAACTCGACAATAAAATAG
AGCAGCTACTAGATGCCACTGGTAGCTTAAATTTAAGTGATGATGAGTTTAAAGAGTTTGCTGAACTAGATTCTGAGCCA
CAAAAAGACCATACGCCAAAAGATGATGACAAAGACGATGCGCCAATCATTGTTTACATCAATAAAATTTTAATGGATGC
CATTAAAAAAGGCGCGTCAGATTTACACTTTGAACCGTACGAGCATAAATACCGCATTCGCTTTCGTATAGACGGAATTT
TGCATGAAGTAGCCAGCCCACCTAATACGCTTTCTACTAAGCTTTCTGCACGTATTAAAGTAATGTCGAGGCTCGATATT
GCTGAAAAACGTAAGCCACAAGATGGCCGAATTAAATTAAAAATTACTGAACGCAAAAGTATCGATTTTCGTGTAAGTAC
TATGCCGACCCTTTGGGGCGAAAAAATAGTAATGCGTATCCTCGATTCATCAAGTGCCATGTTAGGTATTGATGTATTAG
GTTATGAACCTGAGCAGAAAAAACTCTACCTCGATGCATTAGAGCAACCACAGGGGATGATTTTAGTTACCGGCCCAACT
GGCTCAGGTAAAACCGTCTCCCTGTATACGGGTTTAAATATACTCAATCAGCCAGAGCGAAATATTAGTACCGCAGAGGA
CCCCGTAGAAATAAACCTTGAAGGGGTTAACCAAGTACAAATAAACCCTAAAGCCGAGATGACTTTTGCAAACGCACTGC
GTGCATTTTTGCGCCAAGATCCTGATGTAGTAATGGTAGGTGAAATACGTGATCTTGAAACCGCTGAAATATCAATTAAG
GCGGCGCAAACGGGTCACTTAGTGTTATCAACACTGCATACAAACTCTGCTCCCGAAACCATTACACGTTTATTAAATAT
GGGCGTACCAGCTTATAACGTGGCAAGCTCTATTAGCTTAATTATAGCCCAGCGATTAGCGCGCCGATTATGTCCTAAGT
GTAAAACGCCAGAGCAACTACCGAGTGAAGAACTTATTCGCCAAGGCTTTAGTGAACAACAAATACAGGATATGGTTTTG
TATGCCCCTAAAGGCTGTGATAGTTGTACCGATGGCTATAAAGGCCGTGTAGGTATTTATGAAGTAATGCAAATTACGCC
AGAAATTGCACAAATTATTATGCGTGGTGGTAACTCATTAGAAATAGCAGAGGTATCCCTTAAAGCTGGGTTTAATAATT
TACGCTTATCGGGTATTCGTAAAGCAGCAGAGGGTGTGACCTCGCTGGCTGAGATAAACCGCGTTACTAGTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-141)

Type II/IV secretion system protein (T2SSE)

(182-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.963

96.774

0.561
  pilB Acinetobacter baylyi ADP1

57.722

92.832

0.536
  pilB Legionella pneumophila strain ERS1305867

55.599

91.219

0.507
  pilB Vibrio cholerae strain A1552

54.231

93.19

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

52.434

95.699

0.502
  pilB Vibrio campbellii strain DS40M4

51.498

95.699

0.493
  pilF Neisseria gonorrhoeae MS11

49.425

93.548

0.462

Multiple sequence alignment