Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACKN8R_RS14855 Genome accession   NZ_CP176031
Coordinates   3202683..3203906 (+) Length   407 a.a.
NCBI ID   WP_005462561.1    Uniprot ID   A0A9P2QS90
Organism   Vibrio parahaemolyticus strain L2171     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3197683..3208906
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKN8R_RS14835 (ACKN8R_14840) ampD 3198707..3199258 (-) 552 WP_029803400.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACKN8R_RS14840 (ACKN8R_14845) nadC 3199351..3200238 (+) 888 WP_025638275.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACKN8R_RS14845 (ACKN8R_14850) pilA 3200502..3200966 (+) 465 WP_025524817.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACKN8R_RS14850 (ACKN8R_14855) pilB 3200973..3202658 (+) 1686 WP_193282266.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACKN8R_RS14855 (ACKN8R_14860) pilC 3202683..3203906 (+) 1224 WP_005462561.1 type II secretion system F family protein Machinery gene
  ACKN8R_RS14860 (ACKN8R_14865) pilD 3203971..3204840 (+) 870 WP_005493974.1 A24 family peptidase Machinery gene
  ACKN8R_RS14865 (ACKN8R_14870) coaE 3204841..3205455 (+) 615 WP_078558749.1 dephospho-CoA kinase -
  ACKN8R_RS14870 (ACKN8R_14875) zapD 3205483..3206223 (+) 741 WP_023624252.1 cell division protein ZapD -
  ACKN8R_RS14875 (ACKN8R_14880) yacG 3206292..3206486 (+) 195 WP_005462546.1 DNA gyrase inhibitor YacG -
  ACKN8R_RS14880 (ACKN8R_14885) rplS 3206886..3207239 (-) 354 WP_005462554.1 50S ribosomal protein L19 -
  ACKN8R_RS14885 (ACKN8R_14890) trmD 3207281..3208024 (-) 744 WP_005462562.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  ACKN8R_RS14890 (ACKN8R_14895) rimM 3208052..3208600 (-) 549 WP_005462552.1 ribosome maturation factor RimM -
  ACKN8R_RS14895 (ACKN8R_14900) rpsP 3208629..3208877 (-) 249 WP_005379962.1 30S ribosomal protein S16 -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45234.41 Da        Isoelectric Point: 10.4368

>NTDB_id=1079415 ACKN8R_RS14855 WP_005462561.1 3202683..3203906(+) (pilC) [Vibrio parahaemolyticus strain L2171]
MKTTTPQLKNFRWKGVNSSGKKTSGQTLAMSEIEVRERLDAQHIKIKKLKKSSISFLTKLSHRVKGKDITVFTRQISTML
VTGVPLVQALKLVSDNHKKAEMKSILMSVTRAVEAGTPMSKAMRTASNHFDPLYTDLIATGEQSGNLAEVFERLATYREK
NEQLRAKVIKALIYPAMVILVALGVSFIMLTKVIPEFEKMFVGFGAELPWFTRQVLDLSAWTQNWSPFIALGSISLFISA
RVLSKRSDSFRLMLNRSVLKFPVLGAVLSKAAIAKFSRTLATSFTAGIPILTSLKTTSKTSGNMHYQLAIEEVYRDTAAG
MPMYVAMRNCNVFPELVLQMVMIGEESGRLDDMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGIVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=1079415 ACKN8R_RS14855 WP_005462561.1 3202683..3203906(+) (pilC) [Vibrio parahaemolyticus strain L2171]
ATGAAAACTACGACACCACAATTAAAGAACTTCCGTTGGAAAGGCGTCAACAGCTCAGGGAAAAAGACCTCAGGTCAAAC
TCTGGCAATGAGCGAAATCGAAGTACGAGAGCGCTTAGATGCCCAGCACATCAAAATCAAAAAGCTAAAGAAAAGCAGCA
TCTCTTTTTTGACCAAACTTAGCCATCGCGTAAAAGGAAAAGACATCACGGTTTTCACTCGTCAAATCTCAACGATGCTA
GTAACTGGTGTGCCACTAGTACAAGCATTGAAATTGGTCTCTGATAACCACAAAAAGGCGGAAATGAAATCCATTTTGAT
GAGTGTAACTCGGGCTGTTGAAGCGGGTACGCCGATGTCCAAAGCGATGCGTACCGCCAGTAACCATTTTGACCCGCTGT
ATACCGACTTGATTGCGACAGGAGAACAATCGGGCAACTTAGCCGAAGTATTCGAACGCTTAGCCACCTACAGGGAAAAA
AATGAGCAGCTTAGAGCCAAAGTGATCAAGGCTCTAATATATCCAGCCATGGTTATTCTAGTTGCACTGGGAGTATCGTT
TATCATGCTAACCAAGGTCATTCCCGAGTTTGAAAAAATGTTTGTTGGCTTTGGTGCTGAATTGCCATGGTTTACAAGGC
AAGTCTTAGATCTTTCCGCTTGGACACAAAACTGGAGCCCATTTATCGCACTTGGTTCCATCAGTTTATTTATATCCGCG
AGAGTACTCTCTAAGCGTTCAGATTCTTTTCGTTTAATGCTCAACCGATCTGTACTTAAGTTTCCAGTCCTTGGGGCGGT
TTTATCGAAAGCCGCTATCGCAAAATTTAGTCGAACACTCGCTACGAGCTTTACGGCGGGCATTCCGATTCTAACCAGTT
TAAAAACCACCTCGAAAACGTCCGGAAATATGCATTATCAATTGGCTATCGAAGAGGTTTATCGCGATACCGCAGCAGGA
ATGCCGATGTATGTTGCAATGCGCAATTGCAACGTGTTCCCTGAGTTGGTGCTGCAAATGGTTATGATCGGCGAAGAATC
CGGCCGACTTGACGATATGCTCAATAAAGTCGCCACAATTTACGAGTTTGAAGTGGACAACACCGTCGATAACCTCAGTA
AAATCTTAGAACCATTAATTATCGTTTTTTTAGGTATCGTTGTCGGTGGCTTAGTCACCGCAATGTACTTGCCAATCTTT
AACTTAATGAGTGTATTGGGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

86.241

100

0.862
  pilC Vibrio cholerae strain A1552

74.321

99.509

0.74
  pilC Acinetobacter baylyi ADP1

42.75

98.28

0.42
  pilC Acinetobacter baumannii D1279779

41.439

99.017

0.41
  pilG Neisseria gonorrhoeae MS11

40.494

99.509

0.403
  pilG Neisseria meningitidis 44/76-A

40.247

99.509

0.4
  pilC Pseudomonas stutzeri DSM 10701

40.909

97.297

0.398
  pilC Legionella pneumophila strain ERS1305867

39.803

100

0.398

Multiple sequence alignment