Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACKN8R_RS00080 Genome accession   NZ_CP176031
Coordinates   14050..15798 (-) Length   582 a.a.
NCBI ID   WP_005488227.1    Uniprot ID   A0A7H5D3I7
Organism   Vibrio parahaemolyticus strain L2171     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 9050..20798
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKN8R_RS00060 (ACKN8R_00060) - 9732..10571 (-) 840 WP_005486862.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  ACKN8R_RS00065 (ACKN8R_00065) - 10641..12158 (-) 1518 WP_017449466.1 SPOR domain-containing protein -
  ACKN8R_RS00070 (ACKN8R_00070) aroB 12211..13311 (-) 1101 WP_193282555.1 3-dehydroquinate synthase -
  ACKN8R_RS00075 (ACKN8R_00075) aroK 13343..13861 (-) 519 WP_005381319.1 shikimate kinase AroK -
  ACKN8R_RS00080 (ACKN8R_00080) pilQ 14050..15798 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  ACKN8R_RS00085 (ACKN8R_00085) pilP 15830..16345 (-) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  ACKN8R_RS00090 (ACKN8R_00090) pilO 16335..16928 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACKN8R_RS00095 (ACKN8R_00095) pilN 16921..17499 (-) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  ACKN8R_RS00100 (ACKN8R_00100) pilM 17483..18499 (-) 1017 WP_005496568.1 type IV pilus biogenesis protein PilM Machinery gene

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63751.85 Da        Isoelectric Point: 4.4353

>NTDB_id=1079385 ACKN8R_RS00080 WP_005488227.1 14050..15798(-) (pilQ) [Vibrio parahaemolyticus strain L2171]
MKMRQGLTRTPRMLTGLFSLWLFLLLAPLAAAEDVQSNALKSIDFRTNKSKDAVIVVELASPAAIVDVKRVQEGLSIDLM
NTSVKDEQLYLLDVKDFATVVESVEVFRDTSTTRLVAYIDEEYTHDYRLTGRYLEITVSKLKPNEKVPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQKLTEEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIAVIREIIESLDIPVK
QVQIEARIVTISEGNMDELGIRWGFTSINGNNTVGGSIENNLATIGLYEGGGDGEGEEGGDGVGIDDFLNVNLAATNPNA
ASIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTPQ
ITPDNRLVLDLSVTQDRPGKVVKTGTGEAMAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFR
RSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=1079385 ACKN8R_RS00080 WP_005488227.1 14050..15798(-) (pilQ) [Vibrio parahaemolyticus strain L2171]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATGCTTACTGGGCTCTTCTCCTTATGGTTGTTTTTACTTCTAGC
CCCTTTAGCAGCAGCAGAAGATGTACAGTCTAATGCGCTCAAGAGCATCGACTTTCGCACTAACAAAAGCAAAGATGCCG
TGATTGTGGTGGAGCTTGCATCCCCGGCGGCTATTGTTGATGTTAAACGTGTTCAAGAAGGATTAAGTATTGATTTAATG
AACACGTCGGTAAAAGACGAACAGCTTTACCTTCTCGACGTGAAAGATTTCGCGACGGTGGTGGAAAGTGTGGAAGTGTT
CCGCGATACCTCCACCACACGATTAGTTGCGTATATCGATGAAGAATACACGCATGACTATCGTTTGACGGGGCGTTATC
TTGAAATCACTGTCAGCAAGCTTAAACCAAATGAAAAAGTGCCAGACAAGAGCATCTTAGAAAAAGAAGGCAAGCTGATC
TCGATTAACTTTCAAGACATTCCTGTGCGTAACGTTTTGCAGCTGATTGCAGATTACAACCAGTTTAACTTGGTGGTTTC
GGACTCTGTCGAAGGCAACCTTACACTGCGTCTTGATGGTGTGCCGTGGCAGCAAGTGCTCGACATCATCTTGCAAGTGA
AAGGGTTAGATAAGCGTGTTGATGGCAACGTGATTTTGGTCGCTCCGACCGATGAGCTGGATTTGCGCGAGAAGCAGCAG
TTAGAAAAACAAAAGCTGACGGAAGAAATGGGGGAGCTTTCATCAGAAATCATCAAAGTCAATTTTGCTAAAGCTTCGGA
TATTGCAGAGATGATCAATGGTGAAGGCAACATTAGCATGTTGTCAGAGCGTGGCAGTATGACCATTGATGAGCGAACCA
ATTCGCTATTAATCAGAGAATTGCCAGAGAACATCGCCGTTATTCGCGAAATCATTGAATCGCTCGATATCCCAGTCAAA
CAAGTACAAATTGAAGCCCGAATTGTGACCATCAGTGAGGGTAACATGGACGAGTTAGGTATTCGTTGGGGCTTCACATC
TATTAATGGTAATAACACCGTTGGTGGCTCTATTGAGAACAACCTAGCGACAATCGGTTTGTACGAAGGTGGTGGTGATG
GCGAAGGTGAAGAGGGTGGTGATGGTGTTGGTATCGATGATTTTCTTAATGTGAATTTAGCCGCGACCAACCCGAATGCG
GCGAGCATTGCGTTCCAAGTGGCAAAGTTAGGTTCTGATACATTATTGGACTTAGAGCTGTCTGCATTGCAGCAAGAATC
AAAAGCAGAAATCATTTCGAGCCCACGATTGATCACGACCAATAAGAAGCCCGCGTACATTGAACAAGGTACCGAAATTC
CTTATCTAGAATCTTCATCGAGTGGTGCAACGTCAGTGACCTTTAAAAAAGCGGTGTTGAGCTTGAAGGTGACACCGCAG
ATCACACCTGACAATCGTTTAGTGTTGGATCTGAGTGTGACCCAAGACCGACCTGGTAAGGTAGTGAAAACGGGTACAGG
AGAGGCAATGGCAATTGATACGCAGCGCATTGGTACCCAAGTTCTGGTGAATAATGGTGAGACGGTTGTGCTCGGTGGTA
TTTTTCAGCACAGCATTACTAACTCAGTGGATAAAGTGCCATTGTTGGGCGATCTTCCGCTATTAGGCGCACTTTTCCGC
CGAAGTTACGAAAACGTTGGCAAAAGTGAGTTGCTAATTTTCGTTACGCCTAAGGTCGTTATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(422-580)

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H5D3I7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

91.924

100

0.919
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

76.408

97.595

0.746
  pilQ Vibrio cholerae strain A1552

76.408

97.595

0.746
  pilQ Legionella pneumophila strain ERS1305867

41.016

94.674

0.388
  pilQ Legionella pneumophila strain Lp02

41.016

94.674

0.388
  pilQ Pseudomonas aeruginosa PAK

40.468

95.533

0.387
  pilQ Acinetobacter baumannii strain A118

37.625

100

0.387
  pilQ Acinetobacter baumannii D1279779

37.625

100

0.387
  comQ Acinetobacter baylyi ADP1

38.25

100

0.383

Multiple sequence alignment