Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACJJU9_RS23210 Genome accession   NZ_CP174501
Coordinates   5169669..5171030 (-) Length   453 a.a.
NCBI ID   WP_407729631.1    Uniprot ID   -
Organism   Pseudomonas helleri strain NF1a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5164669..5176030
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJJU9_RS23195 (ACJJU9_23195) - 5166364..5167941 (-) 1578 WP_407729629.1 AAA family ATPase -
  ACJJU9_RS23200 (ACJJU9_23200) aroB 5167952..5169052 (-) 1101 WP_407729630.1 3-dehydroquinate synthase -
  ACJJU9_RS23205 (ACJJU9_23205) aroK 5169146..5169664 (-) 519 WP_048366711.1 shikimate kinase AroK -
  ACJJU9_RS23210 (ACJJU9_23210) pilQ 5169669..5171030 (-) 1362 WP_407729631.1 type IV pilus secretin PilQ Machinery gene
  ACJJU9_RS23215 (ACJJU9_23215) - 5171043..5171573 (-) 531 WP_048386381.1 pilus assembly protein PilP -
  ACJJU9_RS23220 (ACJJU9_23220) - 5171579..5172190 (-) 612 WP_407729632.1 type 4a pilus biogenesis protein PilO -
  ACJJU9_RS23225 (ACJJU9_23225) - 5172190..5172756 (-) 567 WP_048386385.1 PilN domain-containing protein -
  ACJJU9_RS23230 (ACJJU9_23230) pilM 5172756..5173727 (-) 972 WP_407729633.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49047.31 Da        Isoelectric Point: 5.0948

>NTDB_id=1077378 ACJJU9_RS23210 WP_407729631.1 5169669..5171030(-) (pilQ) [Pseudomonas helleri strain NF1a]
MKRIFPVCALALWSALNSQTALAIAQHQPPLSVKPPVVAPPPVYVGEKVSLNFQAIEVRTVLQLLAEFTRFNLVVSDAVQ
GSITLNLQEVPWDQALDLVLASKDLGKRLEGNVLLIAPAEEIAARERHELEAHNQRAELAPLRRELVQVNYAKASDIAKL
FQSVTHGQDGMGLRGSITVDERTNNIIALQSDEQLAELRRIVAQLDVPVRQVMIEARIVEAGVDFERHLGVRWGGTLNAG
GNWSVGGTGAGAGEMSAPFVDMGVRGNTSGLGIAFISNNVLLDLELSAMEKTGNGEIISQPKVVTSDKETAKILKGTEIP
YQESSASGATSVSFKEASLSLEVTPQITPDNSVIMDVVVTKDEPDYMNMVNDVPPIKKNEVNAKVLVKNGETIVIGGVFS
NTQSNVVDKVPFLGDLPYVGRLFRKDVVLEKKSELLVFLTPRIMSNQAIAVSH

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=1077378 ACJJU9_RS23210 WP_407729631.1 5169669..5171030(-) (pilQ) [Pseudomonas helleri strain NF1a]
ATGAAAAGGATTTTCCCGGTCTGTGCACTGGCGCTATGGAGTGCGCTGAATTCGCAGACAGCGCTGGCCATCGCCCAGCA
TCAACCCCCGTTGTCAGTAAAACCCCCTGTAGTGGCGCCGCCCCCGGTGTATGTCGGGGAAAAGGTCTCGCTGAATTTTC
AGGCCATTGAAGTGCGCACGGTGTTGCAGTTATTGGCCGAATTTACCCGGTTCAATCTGGTGGTCAGTGATGCGGTGCAA
GGCAGCATTACCCTCAATTTGCAGGAGGTGCCGTGGGATCAGGCGCTGGACCTGGTGCTGGCCAGCAAAGACCTCGGTAA
GCGTCTCGAAGGCAACGTGCTGTTGATTGCCCCCGCAGAAGAAATCGCCGCCCGTGAGCGCCACGAGTTGGAGGCGCACA
ACCAGCGGGCTGAGCTGGCGCCGTTGCGACGCGAATTGGTGCAGGTCAATTACGCCAAGGCCAGTGACATCGCCAAGCTG
TTCCAGTCCGTTACCCATGGCCAGGACGGCATGGGCCTTCGCGGGTCTATCACCGTGGATGAGCGCACCAATAACATCAT
CGCGCTGCAGTCGGACGAGCAGTTGGCCGAACTGCGGCGCATCGTGGCGCAACTGGACGTGCCGGTTCGTCAGGTCATGA
TCGAAGCGCGCATTGTTGAGGCCGGGGTGGATTTTGAACGCCATTTGGGGGTGCGCTGGGGTGGCACCTTGAATGCGGGT
GGCAACTGGAGTGTCGGCGGCACGGGCGCCGGGGCGGGCGAGATGAGTGCGCCCTTTGTCGACATGGGTGTCAGGGGCAA
TACCTCGGGCTTGGGCATCGCCTTTATCAGCAACAACGTCTTGCTGGACCTTGAGTTGAGCGCCATGGAAAAAACCGGCA
ATGGCGAAATCATCTCTCAGCCCAAAGTGGTCACGTCCGACAAGGAGACCGCCAAAATCCTTAAAGGCACTGAAATTCCT
TACCAGGAGTCCAGTGCCAGCGGCGCCACCTCCGTGTCATTCAAAGAGGCCTCCCTGTCGCTGGAGGTGACCCCGCAAAT
CACCCCGGACAACAGCGTCATCATGGACGTGGTGGTGACCAAGGACGAGCCGGACTACATGAACATGGTCAATGATGTGC
CGCCGATCAAAAAGAACGAGGTCAACGCCAAGGTGCTGGTCAAAAATGGCGAAACCATCGTAATCGGCGGTGTTTTCTCA
AATACTCAAAGCAATGTGGTAGATAAAGTGCCATTTTTGGGCGATCTGCCGTATGTTGGCCGACTTTTCCGTAAAGACGT
TGTGCTGGAAAAAAAATCCGAGCTGCTGGTGTTCCTTACTCCGCGTATTATGAGCAACCAGGCGATTGCTGTGAGTCATT
GA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(71-118)

Bacterial type II and III secretion system protein (Secretin)

(288-444)

Bacterial type II/III secretion system short domain (Secretin_N)

(146-211)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

68.421

96.468

0.66
  comQ Acinetobacter baylyi ADP1

46.137

100

0.461
  pilQ Acinetobacter baumannii D1279779

43.129

100

0.45
  pilQ Acinetobacter baumannii strain A118

43.129

100

0.45
  pilQ Vibrio campbellii strain DS40M4

44.496

94.26

0.419
  pilQ Vibrio cholerae strain A1552

43.953

94.923

0.417
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.953

94.923

0.417
  pilQ Legionella pneumophila strain Lp02

43.705

92.936

0.406
  pilQ Legionella pneumophila strain ERS1305867

43.675

92.494

0.404

Multiple sequence alignment