Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACJD0W_RS12355 Genome accession   NZ_CP174456
Coordinates   2736207..2737892 (+) Length   561 a.a.
NCBI ID   WP_010450185.1    Uniprot ID   A0A2K7SNG8
Organism   Vibrio rotiferianus strain BD182406     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2731207..2742892
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJD0W_RS12335 pdhR 2732818..2733585 (-) 768 WP_005446261.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  ACJD0W_RS12340 ampD 2733990..2734541 (-) 552 WP_282065240.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACJD0W_RS12345 nadC 2734634..2735521 (+) 888 WP_407832468.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACJD0W_RS12350 - 2735749..2736207 (+) 459 WP_045393855.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACJD0W_RS12355 pilB 2736207..2737892 (+) 1686 WP_010450185.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACJD0W_RS12360 pilC 2737908..2739131 (+) 1224 WP_071235305.1 type II secretion system F family protein Machinery gene
  ACJD0W_RS12365 pilD 2739202..2740071 (+) 870 WP_071235304.1 A24 family peptidase Machinery gene
  ACJD0W_RS12370 coaE 2740072..2740686 (+) 615 WP_407832469.1 dephospho-CoA kinase -
  ACJD0W_RS12375 zapD 2740715..2741455 (+) 741 WP_407832470.1 cell division protein ZapD -
  ACJD0W_RS12380 yacG 2741519..2741713 (+) 195 WP_010450195.1 DNA gyrase inhibitor YacG -
  ACJD0W_RS12385 rplS 2742283..2742636 (-) 354 WP_005379971.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62106.11 Da        Isoelectric Point: 4.9193

>NTDB_id=1077124 ACJD0W_RS12355 WP_010450185.1 2736207..2737892(+) (pilB) [Vibrio rotiferianus strain BD182406]
MLTNLCSVLRQAGLIDTQQEDAVIELVKASGISVPEALIHNMYFTSDELAVHLSSLFGLNYCSLCEFDFEPLCKELGLRE
LITKYNALPLKKDSNALTLAIPDPTMWHAEDDFRFATGLQVELVLADFKELQASIRRLYGRSIENSHSNLKDINQDELAN
LVDIGEDEIENIEDLSEDQSPVSRYINQILLDAVRKGASDIHFEPYENIFRVRLRCDGILVETQQPPSHLSRRLSARVKI
LSKLDIAERRLPQDGRIKLKLNSDVAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSTEQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLNILNQPEVNISTAEDPVEINLSGINQVQVQPKIGFTFAEALRSFLRQDPDIVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVVRLNNMGIASFNLASSLSLIIAQRLARRLCQNCKQPASMTPALSSLGISDKDH
VFDANPVGCSDCTNGYSGRTGIYEVMPFDDAMSEAIIKGYTSLELERLAVERGMKTLKASGLEKLKQGITSFSELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1077124 ACJD0W_RS12355 WP_010450185.1 2736207..2737892(+) (pilB) [Vibrio rotiferianus strain BD182406]
ATGCTCACCAACCTCTGCTCTGTTCTTCGTCAGGCTGGTTTAATTGATACTCAGCAAGAAGATGCCGTCATTGAGCTAGT
AAAAGCCTCTGGTATTTCGGTACCGGAGGCTTTGATCCATAACATGTATTTTACCTCAGATGAACTAGCGGTTCATTTGA
GCTCTTTGTTTGGCTTAAACTATTGCTCATTATGCGAGTTTGACTTTGAACCACTTTGTAAAGAGTTGGGGTTAAGAGAG
CTCATTACAAAATATAATGCTCTCCCTCTAAAAAAAGACTCCAATGCTCTTACGCTAGCAATTCCCGACCCCACAATGTG
GCATGCGGAAGATGATTTTAGGTTCGCTACAGGATTGCAAGTGGAGCTAGTCCTTGCAGACTTCAAAGAGCTTCAAGCTT
CTATACGTCGCTTATATGGCAGGTCAATTGAAAACTCTCACTCAAACTTAAAAGATATCAATCAAGACGAACTGGCTAAC
CTTGTAGATATTGGTGAAGACGAAATAGAAAACATCGAAGATTTAAGTGAGGATCAATCCCCAGTCAGCCGTTACATCAA
CCAAATTTTGCTCGACGCAGTCCGGAAAGGAGCTTCCGACATTCATTTTGAGCCATATGAAAACATCTTTCGTGTTCGGC
TACGTTGTGATGGCATCTTAGTTGAAACTCAACAGCCCCCTAGCCATCTTAGCCGTAGACTCTCTGCACGCGTAAAAATT
CTCTCTAAACTTGATATTGCAGAACGACGGTTACCACAAGATGGCCGAATTAAGCTCAAGTTAAATAGTGATGTCGCCAT
TGATATGCGCGTATCTACGCTCCCTACGTTATTTGGCGAAAAAATAGTATTGCGCCTATTGGATAGCAGCTCCGCATCTT
TAGATATTGATAAGCTCGGATACAGTACAGAGCAGAAAGCTTTATATTTGAATGCCTTAAAACGCCCTCAAGGTATGATT
CTTATGACCGGCCCCACCGGTAGTGGAAAAACGGTGTCACTGTATACCGGGTTAAACATTCTAAACCAACCAGAAGTTAA
TATTTCTACAGCGGAAGATCCTGTTGAAATTAACTTATCTGGCATCAATCAAGTGCAAGTTCAACCAAAAATTGGTTTTA
CATTTGCAGAAGCACTGCGTTCATTTTTAAGGCAAGACCCTGATATTGTCATGGTTGGTGAAATACGAGATTTAGAAACT
GCCGAAATAGCAATAAAAGCCTCCCAAACTGGCCACCTCGTTCTATCAACACTGCATACAAATTCCGCTGCCGAAACGGT
TGTTCGCTTAAATAATATGGGCATTGCAAGCTTTAACCTAGCTTCCTCTCTAAGCCTTATTATTGCTCAACGTCTCGCAA
GACGTTTATGCCAAAACTGCAAGCAGCCGGCGTCAATGACTCCTGCGTTATCCTCACTGGGTATATCTGACAAAGACCAT
GTTTTTGACGCCAACCCTGTAGGGTGTAGTGATTGTACAAATGGTTACAGTGGGCGCACAGGGATTTATGAAGTAATGCC
ATTCGATGATGCAATGTCAGAGGCAATCATCAAAGGGTATACATCTCTGGAGCTTGAACGATTAGCTGTAGAGCGAGGAA
TGAAAACACTAAAGGCTTCAGGGTTAGAAAAACTAAAACAAGGGATCACCAGTTTTAGCGAGCTTCAACGCGTACTTTAC
TTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K7SNG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

77.005

100

0.77
  pilB Vibrio campbellii strain DS40M4

76.649

100

0.766
  pilB Vibrio cholerae strain A1552

70.745

100

0.711
  pilB Acinetobacter baylyi ADP1

50.269

99.287

0.499
  pilB Acinetobacter baumannii D1279779

51.692

94.831

0.49
  pilB Legionella pneumophila strain ERS1305867

48.463

98.574

0.478
  pilF Neisseria gonorrhoeae MS11

45.018

100

0.451
  pilF Thermus thermophilus HB27

37.367

100

0.374
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.097

99.465

0.369
  pilB/pilB1 Synechocystis sp. PCC 6803

37.004

98.752

0.365

Multiple sequence alignment