Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACJEHS_RS02020 Genome accession   NZ_CP174225
Coordinates   457755..458975 (+) Length   406 a.a.
NCBI ID   WP_010947252.1    Uniprot ID   Q5ZVB7
Organism   Legionella pneumophila strain SPF641     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 452755..463975
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJEHS_RS01995 (ACJEHS_01995) - 453676..453900 (+) 225 WP_408071664.1 EAL domain-containing protein -
  ACJEHS_RS02000 (ACJEHS_02000) - 453902..454471 (-) 570 WP_010947248.1 hypoxanthine-guanine phosphoribosyltransferase -
  ACJEHS_RS02005 (ACJEHS_02005) - 454514..454840 (-) 327 WP_010947249.1 DUF1820 family protein -
  ACJEHS_RS02010 (ACJEHS_02010) - 455023..455766 (-) 744 WP_010947250.1 methyltransferase domain-containing protein -
  ACJEHS_RS02015 (ACJEHS_02015) pilB 456025..457749 (+) 1725 WP_010947251.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACJEHS_RS02020 (ACJEHS_02020) pilC 457755..458975 (+) 1221 WP_010947252.1 type II secretion system F family protein Machinery gene
  ACJEHS_RS02025 (ACJEHS_02025) pilD 459021..459884 (+) 864 WP_015444491.1 A24 family peptidase Machinery gene
  ACJEHS_RS02030 (ACJEHS_02030) - 459898..461103 (-) 1206 WP_015444490.1 cyclic nucleotide-binding domain-containing protein -
  ACJEHS_RS02035 (ACJEHS_02035) - 461211..461804 (-) 594 WP_010947255.1 hypothetical protein -
  ACJEHS_RS02040 (ACJEHS_02040) - 461820..462275 (-) 456 WP_010947256.1 hypothetical protein -
  ACJEHS_RS02045 (ACJEHS_02045) - 462409..463224 (+) 816 WP_010947257.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44707.87 Da        Isoelectric Point: 9.6052

>NTDB_id=1075658 ACJEHS_RS02020 WP_010947252.1 457755..458975(+) (pilC) [Legionella pneumophila strain SPF641]
MDKNSPPLLTFHYQGINKAGQKMEGDIQARSLAIAKADLRKQGIVTNKVVKKRKPLFDRKNKKITQADITVFSRQLATMI
ESGIPLVQAFDIVAKGQSNKRLKDLIETIKHDIETGLTLAESLIKHPLYFNELFCNLVDAGEKSGSLDIMLDKVATYKEK
IEIIKKKIKKALTYPIAVMVVALLVTAGLLIYVVPQFESLFKGFGADLPAMTRAVITMSEFMQAYWYIIFGALGGIVYSF
FYAKNHSLEFAQTIDRVMLKFPVIGPILEKAAIARFARTLSITFAAGLPLVEALKSVAGATGNIIYAKATDKIREEVATG
QQMFTAMENTHLFPNMVIQMVAIGEESGALEKMLSKVADFYEEEVNNAVDALSSLLEPIIMSILGILVGGLVVAMYLPIF
KLGSAV

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=1075658 ACJEHS_RS02020 WP_010947252.1 457755..458975(+) (pilC) [Legionella pneumophila strain SPF641]
ATGGATAAAAATAGCCCACCCCTATTAACCTTTCATTATCAAGGCATTAATAAAGCCGGTCAAAAAATGGAGGGTGATAT
TCAAGCGAGAAGCTTGGCAATAGCTAAAGCTGATTTGCGTAAGCAGGGGATTGTTACCAATAAAGTAGTAAAAAAAAGAA
AACCTTTGTTTGATAGAAAAAATAAAAAAATCACACAAGCAGATATTACTGTGTTTAGCCGCCAATTAGCGACGATGATC
GAGTCAGGAATACCATTAGTGCAAGCATTTGATATTGTTGCCAAAGGACAAAGCAATAAAAGACTTAAAGACTTAATAGA
AACAATTAAGCACGATATCGAAACGGGGCTAACGCTGGCAGAATCCTTAATTAAACATCCACTCTATTTTAATGAATTAT
TTTGTAATCTTGTCGATGCTGGTGAAAAATCAGGCTCATTAGATATCATGCTTGACAAGGTTGCAACGTATAAAGAAAAA
ATTGAAATCATAAAAAAGAAAATAAAAAAAGCTCTCACTTACCCAATTGCTGTTATGGTTGTCGCACTTTTGGTTACTGC
GGGATTATTAATCTATGTGGTTCCTCAATTTGAATCGTTGTTTAAAGGATTTGGAGCTGATTTGCCAGCGATGACTAGAG
CTGTGATCACTATGTCTGAATTTATGCAGGCTTATTGGTATATTATTTTTGGAGCTTTGGGTGGGATTGTCTATAGCTTT
TTTTATGCTAAAAATCATTCTTTAGAGTTTGCGCAAACCATTGACAGAGTGATGCTAAAATTCCCTGTAATTGGCCCTAT
TTTAGAGAAAGCAGCAATTGCTAGATTTGCGAGGACATTATCCATTACTTTTGCTGCTGGATTACCCCTGGTTGAAGCAT
TGAAATCTGTAGCTGGAGCGACCGGGAATATTATTTATGCCAAGGCGACTGATAAAATCAGAGAAGAAGTTGCAACAGGA
CAGCAAATGTTTACTGCTATGGAAAATACTCATTTATTTCCAAATATGGTAATTCAAATGGTAGCAATTGGTGAGGAATC
AGGTGCCCTTGAAAAAATGTTAAGCAAAGTGGCTGATTTTTATGAGGAAGAAGTAAATAATGCTGTAGATGCCCTGAGTA
GTTTATTGGAGCCGATAATTATGTCAATATTGGGTATACTGGTTGGGGGATTGGTGGTGGCTATGTATTTACCTATTTTC
AAACTGGGTTCTGCTGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5ZVB7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

99.261

100

0.993
  pilC Pseudomonas stutzeri DSM 10701

54.208

99.507

0.539
  pilC Acinetobacter baumannii D1279779

53.333

99.754

0.532
  pilC Acinetobacter baylyi ADP1

52.84

99.754

0.527
  pilG Neisseria meningitidis 44/76-A

45.088

97.783

0.441
  pilG Neisseria gonorrhoeae MS11

44.836

97.783

0.438
  pilC Vibrio cholerae strain A1552

42

98.522

0.414
  pilC Vibrio campbellii strain DS40M4

38.916

100

0.389

Multiple sequence alignment