Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACJEHS_RS02015 Genome accession   NZ_CP174225
Coordinates   456025..457749 (+) Length   574 a.a.
NCBI ID   WP_010947251.1    Uniprot ID   -
Organism   Legionella pneumophila strain SPF641     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 451025..462749
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJEHS_RS01990 (ACJEHS_01990) - 451370..453640 (+) 2271 WP_408071640.1 EAL domain-containing protein -
  ACJEHS_RS01995 (ACJEHS_01995) - 453676..453900 (+) 225 WP_408071664.1 EAL domain-containing protein -
  ACJEHS_RS02000 (ACJEHS_02000) - 453902..454471 (-) 570 WP_010947248.1 hypoxanthine-guanine phosphoribosyltransferase -
  ACJEHS_RS02005 (ACJEHS_02005) - 454514..454840 (-) 327 WP_010947249.1 DUF1820 family protein -
  ACJEHS_RS02010 (ACJEHS_02010) - 455023..455766 (-) 744 WP_010947250.1 methyltransferase domain-containing protein -
  ACJEHS_RS02015 (ACJEHS_02015) pilB 456025..457749 (+) 1725 WP_010947251.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACJEHS_RS02020 (ACJEHS_02020) pilC 457755..458975 (+) 1221 WP_010947252.1 type II secretion system F family protein Machinery gene
  ACJEHS_RS02025 (ACJEHS_02025) pilD 459021..459884 (+) 864 WP_015444491.1 A24 family peptidase Machinery gene
  ACJEHS_RS02030 (ACJEHS_02030) - 459898..461103 (-) 1206 WP_015444490.1 cyclic nucleotide-binding domain-containing protein -
  ACJEHS_RS02035 (ACJEHS_02035) - 461211..461804 (-) 594 WP_010947255.1 hypothetical protein -
  ACJEHS_RS02040 (ACJEHS_02040) - 461820..462275 (-) 456 WP_010947256.1 hypothetical protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62728.28 Da        Isoelectric Point: 5.5861

>NTDB_id=1075657 ACJEHS_RS02015 WP_010947251.1 456025..457749(+) (pilB) [Legionella pneumophila strain SPF641]
MALATEEYRLQGIGQLLVLEKLLDKTKAIELHKLAAAEKMSLLQYIVKNKILSAEQIALTAAQNFGVPMLDINCIDVGTI
PANLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKEIQFHTGLNTHAIVVETDKLSALIDNLLTAKESQGLSEYVE
DSGDLEGLEISADDEDQDIDTATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLS
SRITARIKVMSNLDISERRIPQDGGFKMKISKSRAIDFRVSTCPTSAGEKVVMRVLDSGTAKLGIEALGFNPVQRTNFLK
AIQRPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIM
VGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKAVRDDFTNQG
LIELGFKEADLVNLKLYKAVGCEQCTSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKE
GITTIEEVNRVTVD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=1075657 ACJEHS_RS02015 WP_010947251.1 456025..457749(+) (pilB) [Legionella pneumophila strain SPF641]
ATGGCTCTAGCTACAGAAGAATATAGATTACAGGGAATCGGCCAGCTTCTCGTGCTGGAAAAGCTGCTAGATAAAACAAA
AGCAATTGAACTACACAAGTTGGCAGCAGCAGAAAAGATGTCTTTGCTGCAATACATTGTAAAAAATAAAATATTATCTG
CTGAACAAATTGCACTGACGGCTGCTCAAAATTTTGGTGTACCCATGTTGGATATTAATTGCATCGATGTGGGCACCATT
CCTGCCAATCTGGTTAATGAGAAATTAATTAAACGTCACGCCATGGTTCCTCTTTTTAGTCGAGGCACTAATTTATATCT
TGCAACAGATGATCCTAGTAAACAAGCTTCATTAAAGGAAATTCAATTTCATACCGGATTAAATACTCATGCGATAGTTG
TAGAAACAGATAAACTTAGTGCTCTGATTGATAACCTATTAACAGCAAAGGAAAGTCAGGGCTTATCAGAGTATGTTGAA
GACTCTGGAGATCTGGAAGGTTTAGAAATAAGTGCTGATGATGAAGATCAAGATATTGATACTGCAACATCAGTAACTGA
TGATGCCCCTATCGTGAAATTCGTCAATAAAATTTTATTGGATGCAATAAGGCAAGGAGCTTCTGATATACACTTTGAGC
CTTATGAACGAGAATACCGCATCAGGTACAGGCAAGATGGTATTTTACATGAGGTTGCTACCCCTCCCGCAAGCTTGTCA
TCTCGTATTACAGCACGTATCAAGGTAATGTCTAATTTGGATATATCAGAACGGCGTATCCCCCAGGATGGTGGCTTCAA
AATGAAAATTTCCAAGTCACGAGCAATTGATTTCAGAGTGAGTACATGTCCAACCTCGGCTGGGGAAAAAGTGGTTATGC
GGGTTCTTGATTCTGGTACTGCTAAATTAGGTATTGAAGCATTAGGATTTAATCCTGTTCAGCGAACAAATTTCCTTAAA
GCAATCCAGCGCCCACAAGGGATGATTTTAGTCACTGGCCCTACTGGTAGTGGTAAAACAGTCACTTTATATACGGCTTT
AAATATATTAAATACAATTGAGGTTAATATTTCAACAGCTGAAGATCCAGTTGAAATCAAAGTACCTGGTATCAATCAGG
TTAATATTAATCCTAAAGCTGGCCTAACCTTTTCCGGTGCATTGCGTTCGTTTCTAAGGCAAGATCCTGATATTATAATG
GTTGGTGAGATACGAGACCTTGAAACAGCAGAGATTGCTGTAAAAGCTGCTCAGACAGGGCATTTGGTTCTTTCTACTCT
GCACACAAACAGTGCCGCTGAAACTTTAAATCGTTTAGTAAACATGGGAATACCAACTTTTAACATCGCGAGTTCGGTTA
CCTTAATTATTGCGCAAAGGCTGGCAAGAAAATTATGCAATCAATGTAAGGCAGTGCGAGATGACTTTACCAATCAAGGT
TTGATTGAACTAGGTTTCAAGGAAGCTGATCTGGTTAATCTTAAATTATACAAAGCAGTTGGTTGCGAGCAATGTACAAG
TGGTTATCGCGGGCGAGTCGGGTTGTTTGAGGTTTTACCGATGACGAAAGAGCTTGGCCAATTGATTATGTCAGGTGGTA
ATTCGCTGGATATATTAAAGTTAGCTCAATCAGAAGGTATGCTGACTATTTTTCAATCGGGTATAGAAAAAGTTAAGGAA
GGAATCACGACCATAGAGGAGGTCAATCGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

99.303

100

0.993
  pilB Acinetobacter baylyi ADP1

49.656

100

0.503
  pilB Acinetobacter baumannii D1279779

50.617

98.78

0.5
  pilB Vibrio cholerae strain A1552

50.741

94.077

0.477
  pilF Neisseria gonorrhoeae MS11

46.82

98.606

0.462
  pilB Vibrio campbellii strain DS40M4

47.397

97.038

0.46
  pilB Vibrio parahaemolyticus RIMD 2210633

48.609

93.902

0.456
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.887

92.16

0.368
  pilF Thermus thermophilus HB27

38.662

93.728

0.362

Multiple sequence alignment