Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   AABA82_RS15020 Genome accession   NZ_AP028930
Coordinates   3400670..3401842 (+) Length   390 a.a.
NCBI ID   WP_338298570.1    Uniprot ID   -
Organism   Pandoraea sputorum strain THI4931     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3395670..3406842
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABA82_RS14985 (THI4931_29680) cgtA 3395774..3396904 (-) 1131 WP_039393308.1 Obg family GTPase CgtA -
  AABA82_RS14990 (THI4931_29690) rpmA 3397054..3397314 (-) 261 WP_039393311.1 50S ribosomal protein L27 -
  AABA82_RS14995 (THI4931_29700) rplU 3397343..3397654 (-) 312 WP_039393314.1 50S ribosomal protein L21 -
  AABA82_RS15000 (THI4931_29710) - 3397982..3398911 (+) 930 WP_224786948.1 polyprenyl synthetase family protein -
  AABA82_RS15010 - 3399199..3399324 (-) 126 WP_257125707.1 hypothetical protein -
  AABA82_RS15015 (THI4931_29730) - 3399323..3400528 (+) 1206 WP_257125708.1 HlyC/CorC family transporter -
  AABA82_RS15020 (THI4931_29740) pilF 3400670..3401842 (+) 1173 WP_338298570.1 GspE/PulE family protein Machinery gene
  AABA82_RS15025 (THI4931_29750) - 3401981..3403207 (+) 1227 WP_338298571.1 type II secretion system F family protein -
  AABA82_RS15030 (THI4931_29760) - 3403204..3404064 (+) 861 WP_338298572.1 A24 family peptidase -
  AABA82_RS15035 (THI4931_29770) coaE 3404061..3404675 (+) 615 WP_338298573.1 dephospho-CoA kinase -
  AABA82_RS15040 (THI4931_29780) zapD 3404851..3405606 (+) 756 WP_039393327.1 cell division protein ZapD -
  AABA82_RS15045 (THI4931_29790) yacG 3405611..3405805 (+) 195 WP_039393329.1 DNA gyrase inhibitor YacG -
  AABA82_RS15050 (THI4931_29800) - 3405942..3406376 (-) 435 WP_150777649.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 390 a.a.        Molecular weight: 41909.12 Da        Isoelectric Point: 7.1647

>NTDB_id=107377 AABA82_RS15020 WP_338298570.1 3400670..3401842(+) (pilF) [Pandoraea sputorum strain THI4931]
MLRPTIASDDALPSIGTPASTTPLQRLEQILSEAIRAGASDIHFEPMAQRFRVRLRVDGRLQEHGDVPLTARDMLASRLK
VLANLDIAQKRLPQDGRMVWREAGEPVECRVSALPTLHGEKLVVRLLDGAKVPLSLEGLGYSPKQYLALTQAIARPHGLI
LLTGPTGSGKTVSLYSCLRRLNDASRNIVTIEDPVEIRLAGITQVNLNERAGLDFATALRAFLRQDPDVLVVGEIRDAQT
AEIAIQAAQTGHLVFATVHANDAPSTLARLFDLGVAPFNLSSTLLLVSAQRLLRRRCPAGCEPSRTTTTETGLADTCMRC
HGTGFAGRIGAFQVMPISATQAINIAQARSPHELAAQACSEGVMTLREAGLWHVDAGAVAQEDVDATLPA

Nucleotide


Download         Length: 1173 bp        

>NTDB_id=107377 AABA82_RS15020 WP_338298570.1 3400670..3401842(+) (pilF) [Pandoraea sputorum strain THI4931]
ATGCTGCGTCCAACCATCGCCTCCGACGACGCCCTGCCGTCCATCGGCACCCCGGCGTCCACCACACCTCTGCAACGCCT
TGAGCAAATACTGAGCGAAGCGATTCGTGCGGGCGCTTCCGATATCCATTTCGAACCGATGGCGCAGCGCTTTCGGGTCC
GTCTGCGTGTCGACGGTCGCTTGCAGGAGCATGGCGACGTTCCGCTGACAGCGCGCGACATGCTGGCCTCGCGTCTGAAG
GTGCTTGCCAATCTCGACATCGCACAAAAGCGCTTGCCGCAGGACGGACGCATGGTGTGGCGCGAAGCTGGTGAACCGGT
CGAGTGTCGCGTGAGTGCGCTTCCCACCCTGCACGGCGAGAAACTCGTGGTGCGCCTGCTCGATGGCGCGAAAGTCCCTC
TCTCGCTTGAGGGTCTCGGCTATTCGCCGAAGCAATACCTCGCACTCACGCAAGCGATTGCCCGGCCACATGGCCTGATC
TTGCTGACGGGCCCAACGGGCAGTGGAAAGACCGTCTCGCTCTATAGTTGCCTGCGACGTCTGAACGACGCGTCGCGCAA
CATCGTCACCATCGAGGACCCGGTGGAAATTCGCTTGGCCGGTATCACGCAGGTCAATCTCAACGAACGCGCCGGCCTCG
ACTTTGCAACGGCTTTACGTGCGTTTCTCCGTCAGGATCCAGATGTCCTCGTCGTGGGCGAAATCCGCGATGCGCAGACC
GCCGAAATCGCCATACAGGCCGCGCAGACCGGTCACCTTGTGTTCGCCACCGTTCACGCCAACGACGCGCCGAGCACGCT
CGCGCGGCTCTTCGATCTCGGTGTCGCCCCGTTCAATCTGTCGTCGACATTACTGCTGGTCAGCGCACAGCGTCTGCTTA
GGCGACGATGCCCTGCCGGGTGCGAGCCGTCCCGCACGACGACGACTGAAACAGGCCTCGCTGACACCTGCATGCGGTGT
CACGGCACCGGTTTTGCCGGACGCATCGGCGCGTTTCAGGTGATGCCGATCAGCGCCACGCAGGCTATCAACATTGCACA
GGCGCGCAGTCCGCACGAGCTGGCGGCGCAGGCATGCAGCGAAGGCGTCATGACGCTGCGAGAAGCCGGGTTATGGCATG
TCGATGCAGGGGCTGTCGCTCAGGAGGATGTCGATGCGACGCTGCCCGCTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(29-295)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

48.718

100

0.487
  pilB Vibrio cholerae strain A1552

47.927

98.974

0.474
  pilB Vibrio parahaemolyticus RIMD 2210633

46.194

97.692

0.451
  pilB Acinetobacter baylyi ADP1

46.053

97.436

0.449
  pilB Vibrio campbellii strain DS40M4

47.297

94.872

0.449
  pilB Legionella pneumophila strain ERS1305867

43.284

100

0.446
  pilB Acinetobacter baumannii D1279779

45

97.436

0.438
  pilB/pilB1 Synechocystis sp. PCC 6803

39.609

100

0.415
  pilF Thermus thermophilus HB27

42.895

95.641

0.41
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.379

96.667

0.4
  pilB Haemophilus influenzae 86-028NP

42.149

93.077

0.392
  pilB Haemophilus influenzae Rd KW20

46.452

79.487

0.369
  pilB Glaesserella parasuis strain SC1401

38.919

94.872

0.369

Multiple sequence alignment