Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   RE428_RS08775 Genome accession   NZ_AP028878
Coordinates   1945850..1947067 (-) Length   405 a.a.
NCBI ID   WP_004581627.1    Uniprot ID   N6WQR5
Organism   Marinobacter nanhaiticus D15-8W     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1940850..1952067
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RE428_RS08750 (RE428_17820) tsaA 1941926..1942666 (-) 741 WP_004581622.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  RE428_RS08755 (RE428_17830) - 1942663..1943733 (-) 1071 WP_227500224.1 hypothetical protein -
  RE428_RS08760 (RE428_17840) - 1943708..1944160 (-) 453 WP_004581624.1 HIT family protein -
  RE428_RS08765 (RE428_17850) coaE 1944227..1944844 (-) 618 WP_004581625.1 dephospho-CoA kinase -
  RE428_RS08770 (RE428_17860) pilD 1944911..1945786 (-) 876 WP_004581626.1 A24 family peptidase Machinery gene
  RE428_RS08775 (RE428_17870) pilC 1945850..1947067 (-) 1218 WP_004581627.1 type II secretion system F family protein Machinery gene
  RE428_RS08780 (RE428_17880) pilB 1947070..1948785 (-) 1716 WP_004581628.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RE428_RS08785 (RE428_17890) - 1949106..1949576 (+) 471 WP_004581629.1 pilin -
  RE428_RS08790 - 1949610..1951151 (+) 1542 WP_154660759.1 O-antigen ligase family protein -
  RE428_RS08795 (RE428_17900) - 1951252..1951512 (-) 261 WP_004581630.1 IS3 family transposase -
  RE428_RS08800 (RE428_17910) - 1951503..1951901 (-) 399 WP_115840248.1 DDE-type integrase/transposase/recombinase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44041.74 Da        Isoelectric Point: 9.6386

>NTDB_id=107256 RE428_RS08775 WP_004581627.1 1945850..1947067(-) (pilC) [Marinobacter nanhaiticus D15-8W]
MAENAQKLSTFVWEGKDRKGNKTNGELAGSSLALVKAQLRKQGIVPSKVRKKPKPLFGGSKKITPFDIAMLTRQLATMMK
AGVPLVQSFDIVADGLDNKGLQELVIAIRNDIAAGTSFAGSLRRHPKYFDDLYCNLVDSGEKAGALEQMLDRIATYLEKT
ETLKKKVKKAMTYPAAVVAVAIVVTAILLVKVVPQFESLFQGFGADLPVFTQFIIRISEWMQAWWFIVLIGIVGAIFAFK
EAKRRSQKFSDLVDKYVLKLPIVGEILDKSAVAKFGRVLSTTFASGVPLVDALESVAGATGNAIYRDAVLRIRDEVSSGT
QLQASMRSAGVFPVMAIQLTAIGEESGNLDSMLEKVADHYESVVDDMVDNLTALMEPMIMAVLGVLVGGLIVGMYLPIFQ
MGQVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=107256 RE428_RS08775 WP_004581627.1 1945850..1947067(-) (pilC) [Marinobacter nanhaiticus D15-8W]
ATGGCAGAAAATGCACAGAAGCTGTCTACCTTTGTATGGGAAGGTAAGGACCGTAAAGGTAACAAGACCAACGGCGAACT
CGCCGGTTCGAGCTTGGCTTTGGTAAAAGCACAGCTAAGAAAGCAGGGGATTGTTCCTAGCAAGGTTAGAAAAAAACCTA
AACCATTGTTCGGCGGAAGCAAGAAGATCACGCCGTTCGATATTGCAATGCTTACGCGTCAATTGGCGACCATGATGAAG
GCGGGCGTACCGCTAGTTCAGAGCTTTGATATCGTAGCAGACGGTCTCGATAATAAAGGACTGCAAGAACTAGTGATCGC
GATCCGTAATGATATCGCTGCAGGTACTAGTTTTGCCGGCTCCCTCCGCCGGCACCCCAAGTACTTTGATGATCTCTACT
GCAACCTTGTTGATTCAGGCGAGAAGGCAGGCGCATTGGAACAAATGCTTGACCGTATCGCCACATACCTTGAGAAAACC
GAGACACTTAAGAAAAAAGTAAAAAAAGCGATGACATATCCAGCTGCTGTCGTCGCGGTGGCGATCGTTGTTACCGCCAT
ACTGCTGGTGAAGGTCGTACCTCAATTCGAATCTCTATTTCAAGGCTTTGGCGCGGACCTACCGGTTTTCACGCAATTTA
TTATTCGAATCTCCGAGTGGATGCAGGCTTGGTGGTTTATTGTACTCATTGGAATAGTTGGAGCTATCTTCGCATTCAAA
GAAGCCAAGAGACGCTCTCAGAAATTTTCTGACTTGGTCGATAAATATGTCTTGAAGCTCCCTATTGTCGGCGAAATTCT
AGATAAATCGGCTGTCGCCAAATTTGGTCGAGTGTTATCTACAACATTCGCATCAGGCGTTCCCCTTGTCGACGCTCTCG
AGTCCGTTGCAGGTGCAACCGGCAACGCGATTTACCGCGATGCTGTACTTCGAATCAGAGATGAAGTTTCAAGTGGTACT
CAGCTTCAAGCATCCATGCGCTCGGCGGGAGTTTTCCCCGTGATGGCAATTCAACTGACCGCCATCGGGGAAGAATCCGG
TAACCTCGATTCCATGCTAGAAAAAGTCGCTGACCACTACGAATCTGTTGTTGACGATATGGTCGACAACCTCACGGCTT
TGATGGAACCGATGATCATGGCCGTTCTCGGGGTGCTGGTCGGCGGTCTAATTGTTGGTATGTACCTGCCAATCTTTCAA
ATGGGCCAGGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-194)

Type II secretion system (T2SS), protein F (T2SSF)

(277-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N6WQR5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

66.173

100

0.662
  pilC Acinetobacter baumannii D1279779

58.765

100

0.588
  pilC Acinetobacter baylyi ADP1

57.635

100

0.578
  pilC Legionella pneumophila strain ERS1305867

55.172

100

0.553
  pilG Neisseria gonorrhoeae MS11

43.035

99.259

0.427
  pilG Neisseria meningitidis 44/76-A

42.786

99.259

0.425
  pilC Vibrio cholerae strain A1552

39.85

98.519

0.393
  pilC Vibrio campbellii strain DS40M4

38.424

100

0.385

Multiple sequence alignment