Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RE428_RS08780 Genome accession   NZ_AP028878
Coordinates   1947070..1948785 (-) Length   571 a.a.
NCBI ID   WP_004581628.1    Uniprot ID   N6WX48
Organism   Marinobacter nanhaiticus D15-8W     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1942070..1953785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RE428_RS08755 (RE428_17830) - 1942663..1943733 (-) 1071 WP_227500224.1 hypothetical protein -
  RE428_RS08760 (RE428_17840) - 1943708..1944160 (-) 453 WP_004581624.1 HIT family protein -
  RE428_RS08765 (RE428_17850) coaE 1944227..1944844 (-) 618 WP_004581625.1 dephospho-CoA kinase -
  RE428_RS08770 (RE428_17860) pilD 1944911..1945786 (-) 876 WP_004581626.1 A24 family peptidase Machinery gene
  RE428_RS08775 (RE428_17870) pilC 1945850..1947067 (-) 1218 WP_004581627.1 type II secretion system F family protein Machinery gene
  RE428_RS08780 (RE428_17880) pilB 1947070..1948785 (-) 1716 WP_004581628.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RE428_RS08785 (RE428_17890) - 1949106..1949576 (+) 471 WP_004581629.1 pilin -
  RE428_RS08790 - 1949610..1951151 (+) 1542 WP_154660759.1 O-antigen ligase family protein -
  RE428_RS08795 (RE428_17900) - 1951252..1951512 (-) 261 WP_004581630.1 IS3 family transposase -
  RE428_RS08800 (RE428_17910) - 1951503..1951901 (-) 399 WP_115840248.1 DDE-type integrase/transposase/recombinase -
  RE428_RS08805 (RE428_17920) - 1951931..1952110 (+) 180 Protein_1746 integrase core domain-containing protein -
  RE428_RS08810 (RE428_17930) rfaH 1952339..1952818 (+) 480 WP_004581633.1 transcription/translation regulatory transformer protein RfaH -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62913.93 Da        Isoelectric Point: 4.7881

>NTDB_id=107257 RE428_RS08780 WP_004581628.1 1947070..1948785(-) (pilB) [Marinobacter nanhaiticus D15-8W]
MPPSNQNVTLTGLARRFVEDGLLDEAAARDAFIQASNNKIPLITYLVQNNLADSRGLAFSAAMEFGVSVLDLSSFAPEML
PEKIVDEKLVRKHNALPLYKRGKRLFIAVSDPTNIQALDEIKFNTGLSTDAVLVDDSRLRELIDRYLESSDTSMGDLDDS
DLEDIDVEGGDDEREDAGVTAADIDDAPIVKYVNKVLLDAIRGGASDIHFEPYEKTYRVRYRTDGILKEMSKPSIKLAPK
ISARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSSAKLGIDALGYEDEQKQMYMEAL
EQPQGMILVTGPTGSGKTVSLYTGLNILNTQERNISTAEDPAEINLEGINQVNVNTKVGLGFAEALRAFLRQDPDVIMVG
EIRDLETANIAIKAAQTGHLVLSTLHTNSAPETLTRMMNMGVPAFNIATSVSLIIAQRLARRLCSCKEPLDVPKDVLLKE
GFTEQQIETGFTLYRPKGCDKCNGGYKGRVGIYEVVKITDELANMIMEEASSIQIAKQAQAEGFANLRQSALKKVIDGMT
SLEEANRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=107257 RE428_RS08780 WP_004581628.1 1947070..1948785(-) (pilB) [Marinobacter nanhaiticus D15-8W]
ATGCCTCCTAGCAATCAGAACGTTACTCTTACCGGGCTTGCACGCCGCTTCGTTGAAGACGGACTGCTGGACGAAGCCGC
TGCTCGCGATGCTTTTATCCAGGCATCCAATAATAAGATCCCGCTCATCACCTATCTTGTGCAAAACAATCTAGCCGATA
GCCGTGGGCTGGCGTTTTCAGCCGCAATGGAGTTTGGTGTTTCGGTACTGGATCTAAGTTCTTTCGCTCCTGAAATGCTG
CCGGAGAAGATCGTCGACGAGAAACTCGTCAGGAAACATAACGCTCTACCACTCTACAAGAGGGGTAAGCGCCTCTTTAT
CGCGGTTTCCGATCCGACAAATATCCAGGCGCTCGATGAGATCAAGTTCAATACCGGCCTGAGTACCGACGCCGTGCTAG
TCGATGACTCTCGACTCAGAGAACTCATTGACCGCTACCTCGAATCCAGCGACACCTCCATGGGAGATCTTGACGACAGC
GATCTTGAAGACATCGATGTGGAGGGCGGGGACGATGAACGTGAAGACGCCGGCGTTACAGCCGCTGACATAGACGATGC
ACCTATCGTCAAGTACGTGAATAAAGTTCTTCTTGACGCTATCCGTGGCGGAGCTTCGGATATTCATTTTGAACCTTATG
AAAAGACCTATCGAGTTCGCTATCGAACCGATGGCATTCTTAAGGAAATGTCCAAGCCCTCCATAAAGTTGGCACCGAAG
ATCTCCGCTCGCGTAAAGATCATGGCTCAATTGGATATTTCAGAGCGCCGCGTACCTCAAGACGGCCGTATCAAGATGAA
GCTGTCCAAGACCAAAGCCATCGACTTCCGGGTAAACACCCTGCCGACACTTTGGGGCGAAAAGATCGTACTGCGAATAT
TGGACCCAAGCTCTGCCAAACTCGGCATTGATGCTCTGGGCTATGAGGATGAACAAAAGCAAATGTATATGGAGGCGCTG
GAACAGCCTCAGGGCATGATTCTGGTTACAGGCCCTACGGGTTCGGGTAAGACAGTCTCGCTCTATACCGGCCTAAATAT
CCTCAATACTCAGGAACGCAATATTTCCACCGCCGAGGATCCGGCGGAAATCAATCTCGAAGGCATCAACCAGGTTAACG
TCAACACCAAGGTTGGCCTCGGCTTCGCTGAAGCCCTTCGCGCATTCCTGCGTCAGGATCCGGACGTCATCATGGTCGGT
GAGATCCGGGATCTGGAAACCGCCAATATAGCTATCAAGGCTGCCCAAACAGGCCACTTGGTGCTCTCGACGCTACATAC
TAACAGCGCCCCAGAAACACTGACCCGAATGATGAACATGGGCGTTCCCGCTTTTAACATTGCAACTTCCGTAAGCTTAA
TTATTGCCCAGCGACTTGCCCGACGCTTATGTAGCTGCAAAGAGCCTCTAGATGTCCCCAAGGACGTTCTTTTGAAGGAA
GGCTTCACAGAGCAACAAATTGAAACAGGCTTTACTTTGTACCGACCCAAGGGCTGTGATAAATGCAATGGTGGATATAA
AGGTCGCGTCGGGATTTACGAAGTGGTGAAGATTACGGATGAGCTGGCCAACATGATCATGGAAGAGGCTAGCTCCATCC
AGATTGCAAAACAGGCTCAGGCGGAAGGCTTCGCAAACTTGAGGCAGTCAGCCTTGAAGAAAGTCATTGACGGCATGACC
AGTCTCGAAGAAGCGAACCGCGTGACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N6WX48

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.275

99.475

0.58
  pilB Acinetobacter baylyi ADP1

57.624

98.774

0.569
  pilB Legionella pneumophila strain ERS1305867

53.819

100

0.543
  pilB Vibrio cholerae strain A1552

53.223

95.096

0.506
  pilB Vibrio parahaemolyticus RIMD 2210633

51.429

98.074

0.504
  pilB Vibrio campbellii strain DS40M4

50.53

99.124

0.501
  pilF Neisseria gonorrhoeae MS11

47.795

99.299

0.475
  pilB/pilB1 Synechocystis sp. PCC 6803

41.233

90.893

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.301

92.995

0.375

Multiple sequence alignment