Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   RE428_RS04800 Genome accession   NZ_AP028878
Coordinates   1047263..1049389 (+) Length   708 a.a.
NCBI ID   WP_040882496.1    Uniprot ID   N6VXL4
Organism   Marinobacter nanhaiticus D15-8W     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1042263..1054389
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RE428_RS04780 (RE428_09610) pilM 1044459..1045526 (+) 1068 WP_004580835.1 pilus assembly protein PilM Machinery gene
  RE428_RS04785 (RE428_09620) - 1045526..1046092 (+) 567 WP_004580836.1 PilN domain-containing protein -
  RE428_RS04790 (RE428_09630) pilO 1046089..1046700 (+) 612 WP_004580837.1 type 4a pilus biogenesis protein PilO -
  RE428_RS04795 (RE428_09640) - 1046703..1047251 (+) 549 WP_040882494.1 pilus assembly protein PilP -
  RE428_RS04800 (RE428_09650) pilQ 1047263..1049389 (+) 2127 WP_040882496.1 type IV pilus secretin PilQ Machinery gene
  RE428_RS04805 (RE428_09660) aroK 1049523..1050089 (+) 567 WP_040882498.1 shikimate kinase AroK -
  RE428_RS04810 (RE428_09670) aroB 1050082..1051170 (+) 1089 WP_004580841.1 3-dehydroquinate synthase -
  RE428_RS04815 (RE428_09680) - 1051218..1052933 (+) 1716 WP_004580842.1 AAA family ATPase -

Sequence

Protein


Download         Length: 708 a.a.        Molecular weight: 76028.27 Da        Isoelectric Point: 4.2605

>NTDB_id=107252 RE428_RS04800 WP_040882496.1 1047263..1049389(+) (pilQ) [Marinobacter nanhaiticus D15-8W]
MNNQRRLVVKKSLGSRLTMYRKIALMFGMAALGMVSGLAQAVSLNDVDFTSLPGDGTEVTLTFDGTPPQPTGYTIERPAR
IAVDLRNTQNALENRSVSLGGGNAQSMTVVETDDRTRLIFNLVELVPYSTDVSGNTVTLRIGGEGSGGSSAKASSNQAMP
ASSTSSSVKGEAVTNVDFRRGANGEGRIIVTLSNPGIPVDLSETGGRIRLVLDDAQVPEQLRRRLDVTDFATPVTRVDTF
MQGDDAVIEIRPEGDYDYLAYQADNEFTVSVEPLTQEEAESRREEKFPYSGEKLSLNFQDIEVRSVLQLIADFTGLNLVA
SDTVSGSITLRLQNVPWDQALDLILKTKGLDKRQIGNVLLVAPADEIAARERLELETSKQIAELAPVRLDIIQVNYAKAA
DIVALIQADEELISGRGFISSDARTNTISVRETAEKLEEIRRLVNTWDIPVRQVSIEARIVRAQTNVAESLGIQWGGAAY
DVSGDNVFSVGGSQGAVEEARDAAGGGSGDISFPGALAVDLGVTGNGASSFAFGWASDDFLVDLELSALETDGKAEVVSQ
PRVVTADRQTASIKSGEEIPYQEASSSGATSVSFKEAVLSLEVTPQITPDNKIIMDLVVNQDSRGEVTAGIPAINTNEVT
TQVLVADGETVVLGGIFQSEVATNITKTPFLGDIPYIGRLFKRTENIDERSELLIFITPSIMRGELIN

Nucleotide


Download         Length: 2127 bp        

>NTDB_id=107252 RE428_RS04800 WP_040882496.1 1047263..1049389(+) (pilQ) [Marinobacter nanhaiticus D15-8W]
ATGAATAATCAAAGAAGACTTGTTGTAAAAAAAAGTTTGGGGTCGAGGCTAACGATGTATAGAAAAATCGCATTAATGTT
CGGCATGGCCGCGCTCGGCATGGTTTCAGGATTGGCGCAAGCCGTCAGCCTGAACGATGTCGATTTTACGTCGCTTCCCG
GCGACGGCACTGAGGTTACCTTGACCTTTGATGGCACACCTCCTCAGCCCACCGGCTATACCATCGAGCGTCCGGCCCGC
ATCGCTGTAGACCTGCGGAATACGCAAAATGCGCTGGAGAATCGCAGCGTGTCGCTGGGCGGTGGTAATGCCCAGAGTAT
GACCGTGGTGGAAACCGATGACCGGACCCGTCTCATATTTAACCTTGTTGAGCTCGTTCCTTATTCTACCGACGTTTCTG
GAAACACAGTTACCCTGAGGATTGGCGGCGAAGGATCAGGTGGTAGTTCTGCAAAGGCGTCGTCCAACCAGGCAATGCCC
GCTTCGAGTACTTCTTCATCGGTGAAGGGAGAGGCAGTCACCAACGTCGATTTCCGCCGTGGTGCGAACGGGGAAGGGCG
CATAATCGTTACCCTTAGCAACCCCGGCATTCCGGTAGATCTCTCTGAGACTGGAGGTCGTATTCGACTGGTGTTGGATG
ATGCCCAGGTGCCCGAACAGCTGCGTCGTCGTCTGGATGTTACCGACTTCGCGACGCCGGTTACCCGCGTGGATACCTTC
ATGCAGGGTGACGACGCTGTCATTGAGATTCGCCCCGAAGGGGATTATGACTATCTGGCATACCAGGCTGACAACGAGTT
TACCGTCAGCGTCGAACCGCTGACGCAGGAAGAAGCTGAAAGCCGTCGTGAAGAGAAGTTCCCCTACAGTGGCGAGAAGC
TGTCGTTGAACTTCCAGGACATCGAAGTACGTTCTGTTCTTCAGCTCATCGCCGATTTCACGGGACTTAACCTCGTAGCC
AGCGATACGGTAAGTGGCAGCATCACGCTGCGCCTGCAGAACGTACCTTGGGACCAGGCTCTTGACCTGATTTTGAAGAC
CAAGGGTTTGGACAAGCGCCAGATCGGCAATGTTCTCCTGGTGGCTCCGGCCGATGAAATCGCTGCGCGCGAGCGTCTGG
AGCTTGAGACCTCCAAGCAGATTGCGGAGTTGGCCCCGGTTCGACTGGACATTATCCAGGTTAATTATGCTAAGGCTGCA
GATATCGTCGCTTTGATCCAGGCTGACGAGGAGCTCATTTCCGGGCGCGGCTTTATTTCTTCGGATGCGCGTACGAATAC
CATCAGCGTAAGAGAGACTGCGGAGAAGCTTGAAGAGATTCGCCGTTTGGTTAACACGTGGGATATTCCTGTGCGCCAGG
TCTCTATCGAGGCCAGAATCGTGCGAGCCCAGACAAACGTCGCCGAGAGTCTTGGTATCCAGTGGGGCGGTGCGGCCTAT
GATGTTAGCGGTGATAACGTGTTTTCCGTAGGTGGCTCTCAGGGTGCTGTTGAAGAGGCTCGCGACGCCGCGGGTGGCGG
CTCTGGGGATATCAGCTTCCCAGGGGCTCTTGCCGTGGATTTGGGGGTGACTGGTAATGGTGCATCGTCGTTTGCCTTTG
GTTGGGCAAGTGATGACTTCCTGGTCGACTTGGAATTGTCCGCCCTGGAAACCGATGGGAAGGCCGAAGTTGTCTCCCAG
CCACGCGTCGTGACTGCTGATCGTCAAACGGCATCCATCAAGTCTGGTGAAGAGATTCCCTATCAGGAAGCTTCATCGAG
TGGCGCGACTTCGGTTTCATTCAAGGAAGCCGTTCTCTCGCTTGAAGTGACTCCGCAGATCACCCCGGACAACAAGATCA
TTATGGATTTGGTTGTGAATCAGGATTCCAGGGGTGAAGTTACAGCGGGTATACCGGCAATCAACACGAATGAGGTGACT
ACCCAGGTCCTTGTAGCCGATGGCGAGACCGTCGTGCTCGGCGGTATCTTCCAGTCGGAGGTAGCCACCAACATCACAAA
GACACCGTTCTTGGGAGATATCCCCTACATTGGCCGCCTATTCAAGCGTACTGAGAATATCGACGAGCGTAGCGAATTGC
TGATCTTTATCACGCCCTCCATCATGCGCGGAGAATTGATCAACTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(317-363)

AMIN domain (AMIN)

(54-139)

Bacterial type II and III secretion system protein (Secretin)

(548-702)

Bacterial type II/III secretion system short domain (Secretin_N)

(391-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N6VXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

52.26

100

0.523
  pilQ Acinetobacter baumannii strain A118

40.685

100

0.419
  pilQ Acinetobacter baumannii D1279779

40.685

100

0.419
  comQ Acinetobacter baylyi ADP1

41.831

100

0.419
  pilQ Legionella pneumophila strain ERS1305867

41.654

95.621

0.398
  pilQ Legionella pneumophila strain Lp02

41.507

95.621

0.397

Multiple sequence alignment